Neuroprotective effect of endophytic fungal antioxidant polyphenols on cerebral ischemic stroke-induced Albino rats; memory impairments, brain damage, and upregulation of metabolic proteins
Background: Stroke is one of the leading causes of mortality and disability throughout the world. Recently, antioxidant therapies were attempted to reduce apoptotic cell death in cerebral ischemia animal model. Purpose: To study the neuroprotective properties of polyphenol derived from fungal endoph...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-01-01
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| Series: | Journal of King Saud University: Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1018364722006140 |
| _version_ | 1811199057066983424 |
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| author | Palanisamy Manikandan Raid AL-Baradie Ahmed Abdelhadi Ayoub Al Othaim Rajendran Vijayakumar Randa Ibrahim Sahar Aldosari Ahmed Alhussain Moorthy Kannaiyan Naif K. Binsaleh Naif Binroshod Alaguraj Veluchamy Khaled A. Al-Gahtany |
| author_facet | Palanisamy Manikandan Raid AL-Baradie Ahmed Abdelhadi Ayoub Al Othaim Rajendran Vijayakumar Randa Ibrahim Sahar Aldosari Ahmed Alhussain Moorthy Kannaiyan Naif K. Binsaleh Naif Binroshod Alaguraj Veluchamy Khaled A. Al-Gahtany |
| author_sort | Palanisamy Manikandan |
| collection | DOAJ |
| description | Background: Stroke is one of the leading causes of mortality and disability throughout the world. Recently, antioxidant therapies were attempted to reduce apoptotic cell death in cerebral ischemia animal model. Purpose: To study the neuroprotective properties of polyphenol derived from fungal endophyte analyzed on experimental Albino rat. Methods: Polyphenols producing endophytic fungi was initially isolated from the seeds of Moringa oleifera Lam. The endophytes were cultured in potato dextrose broth and the potent strain Simplicillium sp. ED7 produced maximum phenolic content (86.42 ± 5.3 mg GAE/g) than other fungi. Polyphenols were extracted with solvent and used for the determination of neuroprotective properties. Results: Isoflurane was used to induce stroke in Albino rat and treated polyphenols showed reduced neurological deficits and improved neuroprotective properties. The ischemic Albino rats treated with polyphenols restored memory loss. The increased dosage of polyphenol improved the biosynthesis of more antioxidant enzymes than lower dosages. Central artery occlusion evoked about 2.28-fold increase in reactive oxygen species in brain tissue and the generation of reactive oxygen species was decreased in polyphenol treated animal. Conclusion: Albino rats treated with different doses of polyphenol had decrease ROS amount than sham group. The elevated level of cytochrome revealed mitochondrial damage in stroke induced control Albino rat. After 24 h of reperfusion on Albino rat, upregulation of total p65 and phospho-p65 were determined. The present finding revealed that polyphenl has a neuroprotective property in ischemia and regulate metabolic enzymes and restore brain injury. |
| first_indexed | 2024-04-12T01:41:28Z |
| format | Article |
| id | doaj.art-2c9e94bec380430d99c53713641e8531 |
| institution | Directory Open Access Journal |
| issn | 1018-3647 |
| language | English |
| last_indexed | 2024-04-12T01:41:28Z |
| publishDate | 2023-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of King Saud University: Science |
| spelling | doaj.art-2c9e94bec380430d99c53713641e85312022-12-22T03:53:11ZengElsevierJournal of King Saud University: Science1018-36472023-01-01351102433Neuroprotective effect of endophytic fungal antioxidant polyphenols on cerebral ischemic stroke-induced Albino rats; memory impairments, brain damage, and upregulation of metabolic proteinsPalanisamy Manikandan0Raid AL-Baradie1Ahmed Abdelhadi2Ayoub Al Othaim3Rajendran Vijayakumar4Randa Ibrahim5Sahar Aldosari6Ahmed Alhussain7Moorthy Kannaiyan8Naif K. Binsaleh9Naif Binroshod10Alaguraj Veluchamy11Khaled A. Al-Gahtany12Department of Medical Laboratory Sciences, College of Applied Medical Sciences, Majmaah University, Al Majmaah 11952, Saudi Arabia; Corresponding author at: Department of Medical Laboratory Sciences, College of Applied Medical Sciences, Majmaah University, Majmaah, Saudi Arabia.Department of Medical Laboratory Sciences, College of Applied Medical Sciences, Majmaah University, Al Majmaah 11952, Saudi ArabiaDepartment of Medical Laboratory Sciences, College of Applied Medical Sciences, Majmaah University, Al Majmaah 11952, Saudi ArabiaDepartment of Medical Laboratory Sciences, College of Applied Medical Sciences, Majmaah University, Al Majmaah 11952, Saudi ArabiaDepartment of Biology, College of Science in Zulfi, Majmaah University, Majmaah 11952, Saudi ArabiaDepartment of Medical Laboratory Sciences, College of Applied Medical Sciences, Majmaah University, Al Majmaah 11952, Saudi ArabiaDepartment of Medical Laboratory Sciences, College of Applied Medical Sciences, Majmaah University, Al Majmaah 11952, Saudi ArabiaDepartment of Medical Laboratory Sciences, College of Applied Medical Sciences, Majmaah University, Al Majmaah 11952, Saudi ArabiaDepartment of Medical Laboratory Science, School of Pharmacy and Medical Laboratory Science, Institute of Health, Post Box Number - 144, Bule Hora University, EthiopiaDepartment of Medical Laboratory Science, College of Applied Medical Sciences, University of Ha’il, Ha’il 55476, Saudi ArabiaEmergncy Medical Services, Prince Sultan bin Abdulaziz College for Emergency Medical Services, King Saud University, Riyadh, 14215, Saudi ArabiaDepartment of Computational Biology, St. Jude Children’s Research Hospital, Danny Thomas Place, Memphis 38105, TN, United States of America; Biological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology, Thuwal 23955, Saudi ArabiaKing Khalid Hospital-Majma'ah, Majmmah 11952, Saudi ArabiaBackground: Stroke is one of the leading causes of mortality and disability throughout the world. Recently, antioxidant therapies were attempted to reduce apoptotic cell death in cerebral ischemia animal model. Purpose: To study the neuroprotective properties of polyphenol derived from fungal endophyte analyzed on experimental Albino rat. Methods: Polyphenols producing endophytic fungi was initially isolated from the seeds of Moringa oleifera Lam. The endophytes were cultured in potato dextrose broth and the potent strain Simplicillium sp. ED7 produced maximum phenolic content (86.42 ± 5.3 mg GAE/g) than other fungi. Polyphenols were extracted with solvent and used for the determination of neuroprotective properties. Results: Isoflurane was used to induce stroke in Albino rat and treated polyphenols showed reduced neurological deficits and improved neuroprotective properties. The ischemic Albino rats treated with polyphenols restored memory loss. The increased dosage of polyphenol improved the biosynthesis of more antioxidant enzymes than lower dosages. Central artery occlusion evoked about 2.28-fold increase in reactive oxygen species in brain tissue and the generation of reactive oxygen species was decreased in polyphenol treated animal. Conclusion: Albino rats treated with different doses of polyphenol had decrease ROS amount than sham group. The elevated level of cytochrome revealed mitochondrial damage in stroke induced control Albino rat. After 24 h of reperfusion on Albino rat, upregulation of total p65 and phospho-p65 were determined. The present finding revealed that polyphenl has a neuroprotective property in ischemia and regulate metabolic enzymes and restore brain injury.http://www.sciencedirect.com/science/article/pii/S1018364722006140StrokeCerebral ischemiaPolyphenolsNeuroprotectiveMetabolic enzymesUpregulation |
| spellingShingle | Palanisamy Manikandan Raid AL-Baradie Ahmed Abdelhadi Ayoub Al Othaim Rajendran Vijayakumar Randa Ibrahim Sahar Aldosari Ahmed Alhussain Moorthy Kannaiyan Naif K. Binsaleh Naif Binroshod Alaguraj Veluchamy Khaled A. Al-Gahtany Neuroprotective effect of endophytic fungal antioxidant polyphenols on cerebral ischemic stroke-induced Albino rats; memory impairments, brain damage, and upregulation of metabolic proteins Journal of King Saud University: Science Stroke Cerebral ischemia Polyphenols Neuroprotective Metabolic enzymes Upregulation |
| title | Neuroprotective effect of endophytic fungal antioxidant polyphenols on cerebral ischemic stroke-induced Albino rats; memory impairments, brain damage, and upregulation of metabolic proteins |
| title_full | Neuroprotective effect of endophytic fungal antioxidant polyphenols on cerebral ischemic stroke-induced Albino rats; memory impairments, brain damage, and upregulation of metabolic proteins |
| title_fullStr | Neuroprotective effect of endophytic fungal antioxidant polyphenols on cerebral ischemic stroke-induced Albino rats; memory impairments, brain damage, and upregulation of metabolic proteins |
| title_full_unstemmed | Neuroprotective effect of endophytic fungal antioxidant polyphenols on cerebral ischemic stroke-induced Albino rats; memory impairments, brain damage, and upregulation of metabolic proteins |
| title_short | Neuroprotective effect of endophytic fungal antioxidant polyphenols on cerebral ischemic stroke-induced Albino rats; memory impairments, brain damage, and upregulation of metabolic proteins |
| title_sort | neuroprotective effect of endophytic fungal antioxidant polyphenols on cerebral ischemic stroke induced albino rats memory impairments brain damage and upregulation of metabolic proteins |
| topic | Stroke Cerebral ischemia Polyphenols Neuroprotective Metabolic enzymes Upregulation |
| url | http://www.sciencedirect.com/science/article/pii/S1018364722006140 |
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