The antibacterial activity of a photoactivatable diarylacetylene against Gram-positive bacteria
The emergence of antibiotic resistance is a growing threat to human health, and therefore, alternatives to existing compounds are urgently needed. In this context, a novel fluorescent photoactivatable diarylacetylene has been identified and characterised for its antibacterial activity, which prefere...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-09-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1243818/full |
| _version_ | 1827809031244218368 |
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| author | Ryan Waite Candace T. Adams David R. Chisholm C. H. Cole Sims Joshua G. Hughes Joshua G. Hughes Joshua G. Hughes Eva Dias Emily A. White Kathryn Welsby Stanley W. Botchway Andrew Whiting Andrew Whiting Gary J. Sharples Carrie A. Ambler Carrie A. Ambler |
| author_facet | Ryan Waite Candace T. Adams David R. Chisholm C. H. Cole Sims Joshua G. Hughes Joshua G. Hughes Joshua G. Hughes Eva Dias Emily A. White Kathryn Welsby Stanley W. Botchway Andrew Whiting Andrew Whiting Gary J. Sharples Carrie A. Ambler Carrie A. Ambler |
| author_sort | Ryan Waite |
| collection | DOAJ |
| description | The emergence of antibiotic resistance is a growing threat to human health, and therefore, alternatives to existing compounds are urgently needed. In this context, a novel fluorescent photoactivatable diarylacetylene has been identified and characterised for its antibacterial activity, which preferentially eliminates Gram-positive over Gram-negative bacteria. Experiments confirmed that the Gram-negative lipopolysaccharide-rich outer surface is responsible for tolerance, as strains with reduced outer membrane integrity showed increased susceptibility. Additionally, bacteria deficient in oxidative damage repair pathways also displayed enhanced sensitivity, confirming that reactive oxygen species production is the mechanism of antibacterial activity. This new diarylacetylene shows promise as an antibacterial agent against Gram-positive bacteria that can be activated in situ, potentially for the treatment of skin infections. |
| first_indexed | 2024-03-11T22:36:39Z |
| format | Article |
| id | doaj.art-2ca0943a02ac4994a295010168375706 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-03-11T22:36:39Z |
| publishDate | 2023-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-2ca0943a02ac4994a2950101683757062023-09-22T12:33:00ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-09-011410.3389/fmicb.2023.12438181243818The antibacterial activity of a photoactivatable diarylacetylene against Gram-positive bacteriaRyan Waite0Candace T. Adams1David R. Chisholm2C. H. Cole Sims3Joshua G. Hughes4Joshua G. Hughes5Joshua G. Hughes6Eva Dias7Emily A. White8Kathryn Welsby9Stanley W. Botchway10Andrew Whiting11Andrew Whiting12Gary J. Sharples13Carrie A. Ambler14Carrie A. Ambler15Department of Biosciences, Durham University, Science Site, Durham, United KingdomLightOx Limited, Newcastle, United KingdomLightOx Limited, Newcastle, United KingdomLightOx Limited, Newcastle, United KingdomDepartment of Biosciences, Durham University, Science Site, Durham, United KingdomLightOx Limited, Newcastle, United KingdomDepartment of Physics, Durham University, Science Site, Durham, United KingdomLightOx Limited, Newcastle, United KingdomDepartment of Biosciences, Durham University, Science Site, Durham, United KingdomCentral Laser Facility, Research Complex at Harwell, Science and Technology Facilities Council, Rutherford Appleton Laboratory, Harwell, United KingdomCentral Laser Facility, Research Complex at Harwell, Science and Technology Facilities Council, Rutherford Appleton Laboratory, Harwell, United KingdomLightOx Limited, Newcastle, United KingdomDepartment of Chemistry, Durham University, Durham, United KingdomDepartment of Biosciences, Durham University, Science Site, Durham, United KingdomDepartment of Biosciences, Durham University, Science Site, Durham, United KingdomLightOx Limited, Newcastle, United KingdomThe emergence of antibiotic resistance is a growing threat to human health, and therefore, alternatives to existing compounds are urgently needed. In this context, a novel fluorescent photoactivatable diarylacetylene has been identified and characterised for its antibacterial activity, which preferentially eliminates Gram-positive over Gram-negative bacteria. Experiments confirmed that the Gram-negative lipopolysaccharide-rich outer surface is responsible for tolerance, as strains with reduced outer membrane integrity showed increased susceptibility. Additionally, bacteria deficient in oxidative damage repair pathways also displayed enhanced sensitivity, confirming that reactive oxygen species production is the mechanism of antibacterial activity. This new diarylacetylene shows promise as an antibacterial agent against Gram-positive bacteria that can be activated in situ, potentially for the treatment of skin infections.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1243818/fullantimicrobial resistancephotodynamic therapyGram-positive bacterialipopolysaccharidesreactive oxygen species |
| spellingShingle | Ryan Waite Candace T. Adams David R. Chisholm C. H. Cole Sims Joshua G. Hughes Joshua G. Hughes Joshua G. Hughes Eva Dias Emily A. White Kathryn Welsby Stanley W. Botchway Andrew Whiting Andrew Whiting Gary J. Sharples Carrie A. Ambler Carrie A. Ambler The antibacterial activity of a photoactivatable diarylacetylene against Gram-positive bacteria Frontiers in Microbiology antimicrobial resistance photodynamic therapy Gram-positive bacteria lipopolysaccharides reactive oxygen species |
| title | The antibacterial activity of a photoactivatable diarylacetylene against Gram-positive bacteria |
| title_full | The antibacterial activity of a photoactivatable diarylacetylene against Gram-positive bacteria |
| title_fullStr | The antibacterial activity of a photoactivatable diarylacetylene against Gram-positive bacteria |
| title_full_unstemmed | The antibacterial activity of a photoactivatable diarylacetylene against Gram-positive bacteria |
| title_short | The antibacterial activity of a photoactivatable diarylacetylene against Gram-positive bacteria |
| title_sort | antibacterial activity of a photoactivatable diarylacetylene against gram positive bacteria |
| topic | antimicrobial resistance photodynamic therapy Gram-positive bacteria lipopolysaccharides reactive oxygen species |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1243818/full |
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