Proteomics‐based identification of the role of osteosarcoma amplified‐9 in hepatocellular carcinoma recurrence
Abstract Hepatocellular carcinoma (HCC) is one of the most prevalent malignancies; its recurrence is associated with high mortality and poor recurrence‐free survival and is affected by multisystem and multilevel pathological changes. To identify the key proteins associated with tumor recurrence and...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Health/LWW
2022-08-01
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| Series: | Hepatology Communications |
| Online Access: | https://doi.org/10.1002/hep4.1952 |
| _version_ | 1811174061569474560 |
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| author | Xuyong Wei Mengfan Yang Binhua Pan Xiaobing Zhang Hanchao Lin Wangyao Li Wenzhi Shu Kun Wang Abdul Rehman Khan Xuanyu Zhang Beini Cen Xiao Xu |
| author_facet | Xuyong Wei Mengfan Yang Binhua Pan Xiaobing Zhang Hanchao Lin Wangyao Li Wenzhi Shu Kun Wang Abdul Rehman Khan Xuanyu Zhang Beini Cen Xiao Xu |
| author_sort | Xuyong Wei |
| collection | DOAJ |
| description | Abstract Hepatocellular carcinoma (HCC) is one of the most prevalent malignancies; its recurrence is associated with high mortality and poor recurrence‐free survival and is affected by multisystem and multilevel pathological changes. To identify the key proteins associated with tumor recurrence and the underlying mechanisms, proteomic profiling of tumor specimens from early recurrence and nonrecurrence patients was performed in this study. Proteomics was applied to identify differentially expressed proteins during the early recurrence of HCC after surgery. Osteosarcoma amplified‐9 (OS‐9) was discovered, and the correlation between OS‐9 expression and the clinicopathological characteristics of patients was analyzed. Invasion and migration were examined in SMMC‐7721 cells with and without OS‐9 overexpression. Proteomics was performed once again using SMMC‐7721 cells with OS‐9 overexpression to further analyze the proteins with altered expression. OS‐9 was overexpressed in the early recurrence group, and OS‐9 overexpression was associated with high serum alpha‐fetoprotein levels and poor recurrence‐free survival in 196 patients with HCC. The invasion and migration abilities of SMMC‐7721 cells were enhanced in the OS‐9 overexpression group. Bioinformatic functional enrichment methods, including Gene Ontology annotation and Kyoto Encyclopedia of Genes and Genomes pathway analysis, revealed that the hypoxia‐inducible factor 1 (HIF‐1) and tumor necrosis factor (TNF) signaling pathways were activated in the OS‐9 overexpression group. The migration and invasion capacities of OS‐9 overexpressed HCC cell line were weakened while treated with HIF‐1α or TNF‐α inhibitors. Conclusion: Our results suggest that the overexpression of OS‐9 is related to HCC recurrence, thereby contributing to the migration and invasion capacities of HCC cell line by regulating the HIF‐1 and TNF pathways. |
| first_indexed | 2024-04-10T17:57:02Z |
| format | Article |
| id | doaj.art-2ca1e2cedd70432c9632ddd6ef7decba |
| institution | Directory Open Access Journal |
| issn | 2471-254X |
| language | English |
| last_indexed | 2024-04-10T17:57:02Z |
| publishDate | 2022-08-01 |
| publisher | Wolters Kluwer Health/LWW |
| record_format | Article |
| series | Hepatology Communications |
| spelling | doaj.art-2ca1e2cedd70432c9632ddd6ef7decba2023-02-02T17:26:08ZengWolters Kluwer Health/LWWHepatology Communications2471-254X2022-08-01682182219710.1002/hep4.1952Proteomics‐based identification of the role of osteosarcoma amplified‐9 in hepatocellular carcinoma recurrenceXuyong Wei0Mengfan Yang1Binhua Pan2Xiaobing Zhang3Hanchao Lin4Wangyao Li5Wenzhi Shu6Kun Wang7Abdul Rehman Khan8Xuanyu Zhang9Beini Cen10Xiao Xu11Department of Hepatobiliary and Pancreatic Surgery Affiliated Hangzhou First People’s Hospital Zhejiang University School of Medicine Hangzhou ChinaDepartment of Hepatobiliary and Pancreatic Surgery Affiliated Hangzhou First People’s Hospital Zhejiang University School of Medicine Hangzhou ChinaDepartment of Hepatobiliary and Pancreatic Surgery Affiliated Hangzhou First People’s Hospital Zhejiang University School of Medicine Hangzhou ChinaDepartment of Hepatobiliary and Pancreatic Surgery Affiliated Hangzhou First People’s Hospital Zhejiang University School of Medicine Hangzhou ChinaDepartment of Hepatobiliary and Pancreatic Surgery Affiliated Hangzhou First People’s Hospital Zhejiang University School of Medicine Hangzhou ChinaDepartment of Hepatobiliary and Pancreatic Surgery Affiliated Hangzhou First People’s Hospital Zhejiang University School of Medicine Hangzhou ChinaDepartment of Hepatobiliary and Pancreatic Surgery Affiliated Hangzhou First People’s Hospital Zhejiang University School of Medicine Hangzhou ChinaDepartment of Hepatobiliary and Pancreatic Surgery Affiliated Hangzhou First People’s Hospital Zhejiang University School of Medicine Hangzhou ChinaDepartment of Hepatobiliary and Pancreatic Surgery Affiliated Hangzhou First People’s Hospital Zhejiang University School of Medicine Hangzhou ChinaNHC Key Laboratory of Combined Multi‐Organ Transplantation Hangzhou ChinaDepartment of Hepatobiliary and Pancreatic Surgery Affiliated Hangzhou First People’s Hospital Zhejiang University School of Medicine Hangzhou ChinaDepartment of Hepatobiliary and Pancreatic Surgery Affiliated Hangzhou First People’s Hospital Zhejiang University School of Medicine Hangzhou ChinaAbstract Hepatocellular carcinoma (HCC) is one of the most prevalent malignancies; its recurrence is associated with high mortality and poor recurrence‐free survival and is affected by multisystem and multilevel pathological changes. To identify the key proteins associated with tumor recurrence and the underlying mechanisms, proteomic profiling of tumor specimens from early recurrence and nonrecurrence patients was performed in this study. Proteomics was applied to identify differentially expressed proteins during the early recurrence of HCC after surgery. Osteosarcoma amplified‐9 (OS‐9) was discovered, and the correlation between OS‐9 expression and the clinicopathological characteristics of patients was analyzed. Invasion and migration were examined in SMMC‐7721 cells with and without OS‐9 overexpression. Proteomics was performed once again using SMMC‐7721 cells with OS‐9 overexpression to further analyze the proteins with altered expression. OS‐9 was overexpressed in the early recurrence group, and OS‐9 overexpression was associated with high serum alpha‐fetoprotein levels and poor recurrence‐free survival in 196 patients with HCC. The invasion and migration abilities of SMMC‐7721 cells were enhanced in the OS‐9 overexpression group. Bioinformatic functional enrichment methods, including Gene Ontology annotation and Kyoto Encyclopedia of Genes and Genomes pathway analysis, revealed that the hypoxia‐inducible factor 1 (HIF‐1) and tumor necrosis factor (TNF) signaling pathways were activated in the OS‐9 overexpression group. The migration and invasion capacities of OS‐9 overexpressed HCC cell line were weakened while treated with HIF‐1α or TNF‐α inhibitors. Conclusion: Our results suggest that the overexpression of OS‐9 is related to HCC recurrence, thereby contributing to the migration and invasion capacities of HCC cell line by regulating the HIF‐1 and TNF pathways.https://doi.org/10.1002/hep4.1952 |
| spellingShingle | Xuyong Wei Mengfan Yang Binhua Pan Xiaobing Zhang Hanchao Lin Wangyao Li Wenzhi Shu Kun Wang Abdul Rehman Khan Xuanyu Zhang Beini Cen Xiao Xu Proteomics‐based identification of the role of osteosarcoma amplified‐9 in hepatocellular carcinoma recurrence Hepatology Communications |
| title | Proteomics‐based identification of the role of osteosarcoma amplified‐9 in hepatocellular carcinoma recurrence |
| title_full | Proteomics‐based identification of the role of osteosarcoma amplified‐9 in hepatocellular carcinoma recurrence |
| title_fullStr | Proteomics‐based identification of the role of osteosarcoma amplified‐9 in hepatocellular carcinoma recurrence |
| title_full_unstemmed | Proteomics‐based identification of the role of osteosarcoma amplified‐9 in hepatocellular carcinoma recurrence |
| title_short | Proteomics‐based identification of the role of osteosarcoma amplified‐9 in hepatocellular carcinoma recurrence |
| title_sort | proteomics based identification of the role of osteosarcoma amplified 9 in hepatocellular carcinoma recurrence |
| url | https://doi.org/10.1002/hep4.1952 |
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