Investigation of Two QTL Conferring Seedling Resistance to <i>Fusarium</i> Crown Rot in Barley on Reducing Grain Yield Loss under Field Environments
<i>Fusarium</i> crown rot (FCR) is one of the most damaging cereal diseases in semi-arid regions worldwide. Genetic studies on FCR resistance have mainly focused on disease symptoms measured by the browning of either leaf sheaths in seedlings or stems of mature plants. Two major QTLs con...
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| Format: | Article |
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MDPI AG
2022-05-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/12/6/1282 |
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| author | Zhi Zheng Jonathan Powell Shang Gao Cassandra Percy Alison Kelly Bethany Macdonald Meixue Zhou Philip Davies Chunji Liu |
| author_facet | Zhi Zheng Jonathan Powell Shang Gao Cassandra Percy Alison Kelly Bethany Macdonald Meixue Zhou Philip Davies Chunji Liu |
| author_sort | Zhi Zheng |
| collection | DOAJ |
| description | <i>Fusarium</i> crown rot (FCR) is one of the most damaging cereal diseases in semi-arid regions worldwide. Genetic studies on FCR resistance have mainly focused on disease symptoms measured by the browning of either leaf sheaths in seedlings or stems of mature plants. Two major QTLs conferring FCR resistance in barley, <i>Qcsr.cpi-1H</i> and <i>Qcrs.cpi-4H</i>, were previously identified in the growth room. They could explain up to 33.4 and 45.3% of phenotypic variance, respectively. This is the first study where the possible effects of FCR-resistant loci identified in the previous studies based on seedling assay are tested for their abilities to reduce grain yield loss. Near isogenic lines (NILs) and backcross (BC) lines targeting these two loci were assessed in the 2017 and 2018 crop seasons. Results from the NILs showed that the presence of a resistance allele at either the 1HL or 4HL locus reduced grain yield loss by an average of 12.0% and 10.7%, respectively. Grain yields of the top BC lines containing resistance alleles at both loci were 34.4% higher than the average of the commercial varieties under FCR inoculation. These lines will be highly valuable in breeding barley varieties with enhanced resistance to FCR. |
| first_indexed | 2024-03-10T00:40:02Z |
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| id | doaj.art-2ca2115b9e714eceaa5d7252458faafe |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-10T00:40:02Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-2ca2115b9e714eceaa5d7252458faafe2023-11-23T15:09:12ZengMDPI AGAgronomy2073-43952022-05-01126128210.3390/agronomy12061282Investigation of Two QTL Conferring Seedling Resistance to <i>Fusarium</i> Crown Rot in Barley on Reducing Grain Yield Loss under Field EnvironmentsZhi Zheng0Jonathan Powell1Shang Gao2Cassandra Percy3Alison Kelly4Bethany Macdonald5Meixue Zhou6Philip Davies7Chunji Liu8Commonwealth Scientific and Industrial Research Organisation (CSIRO) Agriculture and Food, 306 Carmody Road, St. Lucia, QLD 4067, AustraliaCommonwealth Scientific and Industrial Research Organisation (CSIRO) Agriculture and Food, 306 Carmody Road, St. Lucia, QLD 4067, AustraliaCommonwealth Scientific and Industrial Research Organisation (CSIRO) Agriculture and Food, 306 Carmody Road, St. Lucia, QLD 4067, AustraliaCentre for Crop Health, University of Southern Queensland, Toowoomba, QLD 4350, AustraliaLeslie Research Facility, Department of Agriculture and Fisheries, Toowoomba, QLD 4350, AustraliaLeslie Research Facility, Department of Agriculture and Fisheries, Toowoomba, QLD 4350, AustraliaTasmanian Institute of Agriculture, University of Tasmania, Prospect, TAS 7250, AustraliaThe Plant Breeding Institute, School of Life and Environmental Sciences, The University of Sydney, Narrabri, NSW 2390, AustraliaCommonwealth Scientific and Industrial Research Organisation (CSIRO) Agriculture and Food, 306 Carmody Road, St. Lucia, QLD 4067, Australia<i>Fusarium</i> crown rot (FCR) is one of the most damaging cereal diseases in semi-arid regions worldwide. Genetic studies on FCR resistance have mainly focused on disease symptoms measured by the browning of either leaf sheaths in seedlings or stems of mature plants. Two major QTLs conferring FCR resistance in barley, <i>Qcsr.cpi-1H</i> and <i>Qcrs.cpi-4H</i>, were previously identified in the growth room. They could explain up to 33.4 and 45.3% of phenotypic variance, respectively. This is the first study where the possible effects of FCR-resistant loci identified in the previous studies based on seedling assay are tested for their abilities to reduce grain yield loss. Near isogenic lines (NILs) and backcross (BC) lines targeting these two loci were assessed in the 2017 and 2018 crop seasons. Results from the NILs showed that the presence of a resistance allele at either the 1HL or 4HL locus reduced grain yield loss by an average of 12.0% and 10.7%, respectively. Grain yields of the top BC lines containing resistance alleles at both loci were 34.4% higher than the average of the commercial varieties under FCR inoculation. These lines will be highly valuable in breeding barley varieties with enhanced resistance to FCR.https://www.mdpi.com/2073-4395/12/6/1282<i>Fusarium</i> crown rothost resistancebarleyfield assessment |
| spellingShingle | Zhi Zheng Jonathan Powell Shang Gao Cassandra Percy Alison Kelly Bethany Macdonald Meixue Zhou Philip Davies Chunji Liu Investigation of Two QTL Conferring Seedling Resistance to <i>Fusarium</i> Crown Rot in Barley on Reducing Grain Yield Loss under Field Environments Agronomy <i>Fusarium</i> crown rot host resistance barley field assessment |
| title | Investigation of Two QTL Conferring Seedling Resistance to <i>Fusarium</i> Crown Rot in Barley on Reducing Grain Yield Loss under Field Environments |
| title_full | Investigation of Two QTL Conferring Seedling Resistance to <i>Fusarium</i> Crown Rot in Barley on Reducing Grain Yield Loss under Field Environments |
| title_fullStr | Investigation of Two QTL Conferring Seedling Resistance to <i>Fusarium</i> Crown Rot in Barley on Reducing Grain Yield Loss under Field Environments |
| title_full_unstemmed | Investigation of Two QTL Conferring Seedling Resistance to <i>Fusarium</i> Crown Rot in Barley on Reducing Grain Yield Loss under Field Environments |
| title_short | Investigation of Two QTL Conferring Seedling Resistance to <i>Fusarium</i> Crown Rot in Barley on Reducing Grain Yield Loss under Field Environments |
| title_sort | investigation of two qtl conferring seedling resistance to i fusarium i crown rot in barley on reducing grain yield loss under field environments |
| topic | <i>Fusarium</i> crown rot host resistance barley field assessment |
| url | https://www.mdpi.com/2073-4395/12/6/1282 |
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