Microfluidic Droplet-Storage Array
A microfluidic droplet-storage array that is capable of the continuous operation of droplet formation, storing, repositioning, retrieving, injecting and restoring is demonstrated. The microfluidic chip comprised four valve-assisted droplet generators and a 3 × 16 droplet-storage array. The integrate...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-06-01
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| Series: | Micromachines |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2072-666X/11/6/608 |
| _version_ | 1797564351928860672 |
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| author | Hoon Suk Rho Han Gardeniers |
| author_facet | Hoon Suk Rho Han Gardeniers |
| author_sort | Hoon Suk Rho |
| collection | DOAJ |
| description | A microfluidic droplet-storage array that is capable of the continuous operation of droplet formation, storing, repositioning, retrieving, injecting and restoring is demonstrated. The microfluidic chip comprised four valve-assisted droplet generators and a 3 × 16 droplet-storage array. The integrated pneumatically actuated microvalves enable the precise control of aqueous phase dispensing, as well as carrier fluid flow path and direction for flexible manipulating water-in-oil droplets in the chip. The size of droplets formed by the valve-assisted droplet generators was validated under various operating conditions such as pressures for introducing solutions and dispensing time. In addition, flexible droplet addressing in the storage array was demonstrated by storing droplets with various numbers and compositions in different storage units as well as rearranging their stored positions. Moreover, serial injections of new droplets into a retrieved droplet from a storage unit was performed to show the potential of the platform in sequential dosing on incubated droplet-based reactors at the desired timeline. The droplet-storage array with great freedom and flexibility in droplet handling could be applied for performing complex chemical and biologic reactions, especially in which incubation and dosing steps are necessary. |
| first_indexed | 2024-03-10T18:56:06Z |
| format | Article |
| id | doaj.art-2ca3fac5f4834bab8ea41b556a22c8e3 |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-03-10T18:56:06Z |
| publishDate | 2020-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micromachines |
| spelling | doaj.art-2ca3fac5f4834bab8ea41b556a22c8e32023-11-20T04:42:54ZengMDPI AGMicromachines2072-666X2020-06-0111660810.3390/mi11060608Microfluidic Droplet-Storage ArrayHoon Suk Rho0Han Gardeniers1Department of Instructive Biomaterials Engineering, MERLN Institute for Technology-Inspired Regenerative Medicine, Maastricht University, 6229 ER Maastricht, The NetherlandsMesoscale Chemical Systems Group, MESA+ Institute, University of Twente, 7522 NB Enschede, The NetherlandsA microfluidic droplet-storage array that is capable of the continuous operation of droplet formation, storing, repositioning, retrieving, injecting and restoring is demonstrated. The microfluidic chip comprised four valve-assisted droplet generators and a 3 × 16 droplet-storage array. The integrated pneumatically actuated microvalves enable the precise control of aqueous phase dispensing, as well as carrier fluid flow path and direction for flexible manipulating water-in-oil droplets in the chip. The size of droplets formed by the valve-assisted droplet generators was validated under various operating conditions such as pressures for introducing solutions and dispensing time. In addition, flexible droplet addressing in the storage array was demonstrated by storing droplets with various numbers and compositions in different storage units as well as rearranging their stored positions. Moreover, serial injections of new droplets into a retrieved droplet from a storage unit was performed to show the potential of the platform in sequential dosing on incubated droplet-based reactors at the desired timeline. The droplet-storage array with great freedom and flexibility in droplet handling could be applied for performing complex chemical and biologic reactions, especially in which incubation and dosing steps are necessary.https://www.mdpi.com/2072-666X/11/6/608microfluidicsdroplet arraymicrovalve |
| spellingShingle | Hoon Suk Rho Han Gardeniers Microfluidic Droplet-Storage Array Micromachines microfluidics droplet array microvalve |
| title | Microfluidic Droplet-Storage Array |
| title_full | Microfluidic Droplet-Storage Array |
| title_fullStr | Microfluidic Droplet-Storage Array |
| title_full_unstemmed | Microfluidic Droplet-Storage Array |
| title_short | Microfluidic Droplet-Storage Array |
| title_sort | microfluidic droplet storage array |
| topic | microfluidics droplet array microvalve |
| url | https://www.mdpi.com/2072-666X/11/6/608 |
| work_keys_str_mv | AT hoonsukrho microfluidicdropletstoragearray AT hangardeniers microfluidicdropletstoragearray |