Isolation and Initial Characterization of A Pure Cultures Capable to Degradation Methyl tert- Butyl Ether (MTBE)
Methyl tert-butyl ether (MTBE), a gasoline octane enhancer, was introduced as a substitute for lead tetraethyl over 30 years ago. Widespread use of MTBE in gasoline, has introduced MTBE into the environment compartments, mostly into the under ground and surface water and water as a second most frequ...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2006-09-01
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| Series: | Iranian Journal of Public Health |
| Subjects: | |
| Online Access: | https://ijph.tums.ac.ir/index.php/ijph/article/view/2161 |
| _version_ | 1818387423371984896 |
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| author | A Nikpey M Nikpey |
| author_facet | A Nikpey M Nikpey |
| author_sort | A Nikpey |
| collection | DOAJ |
| description | Methyl tert-butyl ether (MTBE), a gasoline octane enhancer, was introduced as a substitute for lead tetraethyl over 30 years ago. Widespread use of MTBE in gasoline, has introduced MTBE into the environment compartments, mostly into the under ground and surface water and water as a second most frequently detected contaminant. In this study, we have isolated pure cultures from bacterial consortium capable to use MTBE as a sole carbon and energy source. MTBE biodegradation rate was measured in headspace by gas chromatography. Initial liner rates of biodegradation by Pinpoint and white strains were found 2.9 mg and 3 mg MTBE hˉ1 gˉ1 wet biomass, respectively. The results of 16S rDNA PCR disclosed similarities in the banding patterns between the cultures, and the known degrading strain PM1. The results of this study suggest promising perspectives for engineering the in situ bioremediation of MTBE. |
| first_indexed | 2024-12-14T04:09:42Z |
| format | Article |
| id | doaj.art-2ca47737282749deaca07a3f27f534c2 |
| institution | Directory Open Access Journal |
| issn | 2251-6085 2251-6093 |
| language | English |
| last_indexed | 2024-12-14T04:09:42Z |
| publishDate | 2006-09-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Public Health |
| spelling | doaj.art-2ca47737282749deaca07a3f27f534c22022-12-21T23:17:43ZengTehran University of Medical SciencesIranian Journal of Public Health2251-60852251-60932006-09-01353Isolation and Initial Characterization of A Pure Cultures Capable to Degradation Methyl tert- Butyl Ether (MTBE) A Nikpey0 M Nikpey1 Methyl tert-butyl ether (MTBE), a gasoline octane enhancer, was introduced as a substitute for lead tetraethyl over 30 years ago. Widespread use of MTBE in gasoline, has introduced MTBE into the environment compartments, mostly into the under ground and surface water and water as a second most frequently detected contaminant. In this study, we have isolated pure cultures from bacterial consortium capable to use MTBE as a sole carbon and energy source. MTBE biodegradation rate was measured in headspace by gas chromatography. Initial liner rates of biodegradation by Pinpoint and white strains were found 2.9 mg and 3 mg MTBE hˉ1 gˉ1 wet biomass, respectively. The results of 16S rDNA PCR disclosed similarities in the banding patterns between the cultures, and the known degrading strain PM1. The results of this study suggest promising perspectives for engineering the in situ bioremediation of MTBE.https://ijph.tums.ac.ir/index.php/ijph/article/view/2161Methyl tert-butyl ether (MTBE)Pure strain16S rDNA |
| spellingShingle | A Nikpey M Nikpey Isolation and Initial Characterization of A Pure Cultures Capable to Degradation Methyl tert- Butyl Ether (MTBE) Iranian Journal of Public Health Methyl tert-butyl ether (MTBE) Pure strain 16S rDNA |
| title | Isolation and Initial Characterization of A Pure Cultures Capable to Degradation Methyl tert- Butyl Ether (MTBE) |
| title_full | Isolation and Initial Characterization of A Pure Cultures Capable to Degradation Methyl tert- Butyl Ether (MTBE) |
| title_fullStr | Isolation and Initial Characterization of A Pure Cultures Capable to Degradation Methyl tert- Butyl Ether (MTBE) |
| title_full_unstemmed | Isolation and Initial Characterization of A Pure Cultures Capable to Degradation Methyl tert- Butyl Ether (MTBE) |
| title_short | Isolation and Initial Characterization of A Pure Cultures Capable to Degradation Methyl tert- Butyl Ether (MTBE) |
| title_sort | isolation and initial characterization of a pure cultures capable to degradation methyl tert butyl ether mtbe |
| topic | Methyl tert-butyl ether (MTBE) Pure strain 16S rDNA |
| url | https://ijph.tums.ac.ir/index.php/ijph/article/view/2161 |
| work_keys_str_mv | AT anikpey isolationandinitialcharacterizationofapureculturescapabletodegradationmethyltertbutylethermtbe AT mnikpey isolationandinitialcharacterizationofapureculturescapabletodegradationmethyltertbutylethermtbe |