The effect of calprotectin on TSLP and IL-25 production from airway epithelial cells
Background: Calprotectin is a heterodimer complex of the S100A8 and S100A9 proteins, and has various functions as an innate mediator at the sites of inflammation. The aim of this study was to elucidate the roles of calprotectin in the eosinophilic chronic rhinosinusitis (ECRS). Methods: Allergen-ind...
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| Format: | Article |
| Language: | English |
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Elsevier
2017-04-01
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| Series: | Allergology International |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1323893016300983 |
| _version_ | 1818195847900299264 |
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| author | Tomohisa Kato Hideaki Kouzaki Koji Matsumoto Junichi Hosoi Takeshi Shimizu |
| author_facet | Tomohisa Kato Hideaki Kouzaki Koji Matsumoto Junichi Hosoi Takeshi Shimizu |
| author_sort | Tomohisa Kato |
| collection | DOAJ |
| description | Background: Calprotectin is a heterodimer complex of the S100A8 and S100A9 proteins, and has various functions as an innate mediator at the sites of inflammation.
The aim of this study was to elucidate the roles of calprotectin in the eosinophilic chronic rhinosinusitis (ECRS).
Methods: Allergen-induced production of calprotectin was evaluated in cultured normal human bronchial epithelial (NHBE) cells by ELISA and RT-PCR. We then examined the roles of calprotectin on Alternaria alternata (Alternaria)-induced production of thymic stromal lymphopoietin (TSLP) and IL-25 in NHBE cells. The extracellular concentration and allergen-induced secretion of calprotectin in cultured primary nasal epithelial (PNE) cells were examined and compared between patients with ECRS and non-eosinophilic chronic rhinosinusitis (NECRS).
Results: Alternaria, house dust mites, protease from Staphylococcus aureus, papain, trypsin, polyinosinic:polycytidylic acid and lipopolysaccharide stimulated calprotectin production in the cultured NHBE cells. The combination of calprotectin and ATP stimulated the production of TSLP and IL-25 in NHBE cells, and calprotectin stimulated Alternaria-induced production of TSLP and IL-25, which was suppressed by blocking P2 purinergic receptors and by treatment with siRNA for S100A8, S100A9 or calprotectin receptors (Toll-like receptor 4 or receptor for advanced glycation end products). Allergen-induced calprotectin production was significantly stimulated in PNE cells from patients with ECRS.
Conclusions: These results indicate that calprotectin enhances the allergen-induced Th2-type inflammatory responses in airway epithelial cells via the secretion of TSLP and IL-25, and that calprotectin secreted by the epithelial cells may be involved in the pathogenesis of ECRS. |
| first_indexed | 2024-12-12T01:24:42Z |
| format | Article |
| id | doaj.art-2ca67a77b2ef4ac5895f9452ba64e9ef |
| institution | Directory Open Access Journal |
| issn | 1323-8930 |
| language | English |
| last_indexed | 2024-12-12T01:24:42Z |
| publishDate | 2017-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Allergology International |
| spelling | doaj.art-2ca67a77b2ef4ac5895f9452ba64e9ef2022-12-22T00:43:07ZengElsevierAllergology International1323-89302017-04-0166228128910.1016/j.alit.2016.06.011The effect of calprotectin on TSLP and IL-25 production from airway epithelial cellsTomohisa Kato0Hideaki Kouzaki1Koji Matsumoto2Junichi Hosoi3Takeshi Shimizu4Department of Otorhinolaryngology, Shiga University of Medical Science, Otsu, JapanDepartment of Otorhinolaryngology, Shiga University of Medical Science, Otsu, JapanDepartment of Otorhinolaryngology, Shiga University of Medical Science, Otsu, JapanShiseido Research Center, Yokohama, JapanDepartment of Otorhinolaryngology, Shiga University of Medical Science, Otsu, JapanBackground: Calprotectin is a heterodimer complex of the S100A8 and S100A9 proteins, and has various functions as an innate mediator at the sites of inflammation. The aim of this study was to elucidate the roles of calprotectin in the eosinophilic chronic rhinosinusitis (ECRS). Methods: Allergen-induced production of calprotectin was evaluated in cultured normal human bronchial epithelial (NHBE) cells by ELISA and RT-PCR. We then examined the roles of calprotectin on Alternaria alternata (Alternaria)-induced production of thymic stromal lymphopoietin (TSLP) and IL-25 in NHBE cells. The extracellular concentration and allergen-induced secretion of calprotectin in cultured primary nasal epithelial (PNE) cells were examined and compared between patients with ECRS and non-eosinophilic chronic rhinosinusitis (NECRS). Results: Alternaria, house dust mites, protease from Staphylococcus aureus, papain, trypsin, polyinosinic:polycytidylic acid and lipopolysaccharide stimulated calprotectin production in the cultured NHBE cells. The combination of calprotectin and ATP stimulated the production of TSLP and IL-25 in NHBE cells, and calprotectin stimulated Alternaria-induced production of TSLP and IL-25, which was suppressed by blocking P2 purinergic receptors and by treatment with siRNA for S100A8, S100A9 or calprotectin receptors (Toll-like receptor 4 or receptor for advanced glycation end products). Allergen-induced calprotectin production was significantly stimulated in PNE cells from patients with ECRS. Conclusions: These results indicate that calprotectin enhances the allergen-induced Th2-type inflammatory responses in airway epithelial cells via the secretion of TSLP and IL-25, and that calprotectin secreted by the epithelial cells may be involved in the pathogenesis of ECRS.http://www.sciencedirect.com/science/article/pii/S1323893016300983CalprotectinEosinophilic chronic rhinosinusitisIL-25Th2-type inflammationTSLP |
| spellingShingle | Tomohisa Kato Hideaki Kouzaki Koji Matsumoto Junichi Hosoi Takeshi Shimizu The effect of calprotectin on TSLP and IL-25 production from airway epithelial cells Allergology International Calprotectin Eosinophilic chronic rhinosinusitis IL-25 Th2-type inflammation TSLP |
| title | The effect of calprotectin on TSLP and IL-25 production from airway epithelial cells |
| title_full | The effect of calprotectin on TSLP and IL-25 production from airway epithelial cells |
| title_fullStr | The effect of calprotectin on TSLP and IL-25 production from airway epithelial cells |
| title_full_unstemmed | The effect of calprotectin on TSLP and IL-25 production from airway epithelial cells |
| title_short | The effect of calprotectin on TSLP and IL-25 production from airway epithelial cells |
| title_sort | effect of calprotectin on tslp and il 25 production from airway epithelial cells |
| topic | Calprotectin Eosinophilic chronic rhinosinusitis IL-25 Th2-type inflammation TSLP |
| url | http://www.sciencedirect.com/science/article/pii/S1323893016300983 |
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