Bone Protective Effect of Extra-Virgin Olive Oil Phenolic Compounds by Modulating Osteoblast Gene Expression
The phenolic compounds of extra-virgin olive oil can act at various levels to protect individuals against cardiovascular and neurodegenerative diseases, cancer, and osteoporosis, among others. Polyphenols in extra-virgin olive oil can stimulate the proliferation of osteoblasts, modify their antigen...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2019-07-01
|
| Series: | Nutrients |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2072-6643/11/8/1722 |
| _version_ | 1811299135801786368 |
|---|---|
| author | Lucía Melguizo-Rodríguez Francisco Javier Manzano-Moreno Rebeca Illescas-Montes Javier Ramos-Torrecillas Elvira de Luna-Bertos Concepción Ruiz Olga García-Martínez |
| author_facet | Lucía Melguizo-Rodríguez Francisco Javier Manzano-Moreno Rebeca Illescas-Montes Javier Ramos-Torrecillas Elvira de Luna-Bertos Concepción Ruiz Olga García-Martínez |
| author_sort | Lucía Melguizo-Rodríguez |
| collection | DOAJ |
| description | The phenolic compounds of extra-virgin olive oil can act at various levels to protect individuals against cardiovascular and neurodegenerative diseases, cancer, and osteoporosis, among others. Polyphenols in extra-virgin olive oil can stimulate the proliferation of osteoblasts, modify their antigen profile, and promote alkaline phosphatase synthesis. The objective of this work was to determine the effect of different extra-virgin olive oil phenolic compounds on the gene expression of osteoblast-related markers. The cells of the MG63 osteoblast line were cultured for 24 h with 10<sup>−6</sup> M of the phenolic compounds ferulic acid, caffeic acid, coumaric acid, apigenin, or luteolin. The expression of studied markers was quantified using quantitative real-time polymerase chain reaction (q-RT-PCR). The expression by MG63 osteoblasts of growth and differentiation/maturation markers was modified after 24 h of treatment with 10<sup>−6</sup> M of the phenolic compounds under study, most of which increased the gene expression of the transforming growth factor β1 (TGF-β1), TGF-β receptor 1,2 and 3 (TGF-βR1, TGF-βR2, TGF-βR3), bone morphogenetic protein 2 and 7 (BMP2, BMP7), run-related transcription factor 2 (RUNX-2), Alkaline phosphatase (ALP), Osteocalcin (OSC), Osterix (OSX), Collagen type I (Col-I) and osteoprotegerin (OPN). The extra-virgin olive oil phenolic compounds may have a beneficial effect on bone by modulating osteoblast physiology, which would support their protective effect against bone pathologies. |
| first_indexed | 2024-04-13T06:30:44Z |
| format | Article |
| id | doaj.art-2ca777c4d58f405db7678b2d1597b74f |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-04-13T06:30:44Z |
| publishDate | 2019-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-2ca777c4d58f405db7678b2d1597b74f2022-12-22T02:58:08ZengMDPI AGNutrients2072-66432019-07-01118172210.3390/nu11081722nu11081722Bone Protective Effect of Extra-Virgin Olive Oil Phenolic Compounds by Modulating Osteoblast Gene ExpressionLucía Melguizo-Rodríguez0Francisco Javier Manzano-Moreno1Rebeca Illescas-Montes2Javier Ramos-Torrecillas3Elvira de Luna-Bertos4Concepción Ruiz5Olga García-Martínez6Biomedical Group (BIO277), Department of Nursing, Faculty of Health Sciences, University of Granada, Avda. Ilustración 60, 18016 Granada, SpainInstituto Investigación Biosanitaria, ibs.Granada, C/ Doctor Azpitarte 4, 4ª planta, 18012 Granada, SpainBiomedical Group (BIO277), Department of Nursing, Faculty of Health Sciences, University of Granada, Avda. Ilustración 60, 18016 Granada, SpainBiomedical Group (BIO277), Department of Nursing, Faculty of Health Sciences, University of Granada, Avda. Ilustración 60, 18016 Granada, SpainBiomedical Group (BIO277), Department of Nursing, Faculty of Health Sciences, University of Granada, Avda. Ilustración 60, 18016 Granada, SpainBiomedical Group (BIO277), Department of Nursing, Faculty of Health Sciences, University of Granada, Avda. Ilustración 60, 18016 Granada, SpainBiomedical Group (BIO277), Department of Nursing, Faculty of Health Sciences, University of Granada, Avda. Ilustración 60, 18016 Granada, SpainThe phenolic compounds of extra-virgin olive oil can act at various levels to protect individuals against cardiovascular and neurodegenerative diseases, cancer, and osteoporosis, among others. Polyphenols in extra-virgin olive oil can stimulate the proliferation of osteoblasts, modify their antigen profile, and promote alkaline phosphatase synthesis. The objective of this work was to determine the effect of different extra-virgin olive oil phenolic compounds on the gene expression of osteoblast-related markers. The cells of the MG63 osteoblast line were cultured for 24 h with 10<sup>−6</sup> M of the phenolic compounds ferulic acid, caffeic acid, coumaric acid, apigenin, or luteolin. The expression of studied markers was quantified using quantitative real-time polymerase chain reaction (q-RT-PCR). The expression by MG63 osteoblasts of growth and differentiation/maturation markers was modified after 24 h of treatment with 10<sup>−6</sup> M of the phenolic compounds under study, most of which increased the gene expression of the transforming growth factor β1 (TGF-β1), TGF-β receptor 1,2 and 3 (TGF-βR1, TGF-βR2, TGF-βR3), bone morphogenetic protein 2 and 7 (BMP2, BMP7), run-related transcription factor 2 (RUNX-2), Alkaline phosphatase (ALP), Osteocalcin (OSC), Osterix (OSX), Collagen type I (Col-I) and osteoprotegerin (OPN). The extra-virgin olive oil phenolic compounds may have a beneficial effect on bone by modulating osteoblast physiology, which would support their protective effect against bone pathologies.https://www.mdpi.com/2072-6643/11/8/1722extra virgin olive oilphenolic compoundsgene expressionosteoblastgenetic profile |
| spellingShingle | Lucía Melguizo-Rodríguez Francisco Javier Manzano-Moreno Rebeca Illescas-Montes Javier Ramos-Torrecillas Elvira de Luna-Bertos Concepción Ruiz Olga García-Martínez Bone Protective Effect of Extra-Virgin Olive Oil Phenolic Compounds by Modulating Osteoblast Gene Expression Nutrients extra virgin olive oil phenolic compounds gene expression osteoblast genetic profile |
| title | Bone Protective Effect of Extra-Virgin Olive Oil Phenolic Compounds by Modulating Osteoblast Gene Expression |
| title_full | Bone Protective Effect of Extra-Virgin Olive Oil Phenolic Compounds by Modulating Osteoblast Gene Expression |
| title_fullStr | Bone Protective Effect of Extra-Virgin Olive Oil Phenolic Compounds by Modulating Osteoblast Gene Expression |
| title_full_unstemmed | Bone Protective Effect of Extra-Virgin Olive Oil Phenolic Compounds by Modulating Osteoblast Gene Expression |
| title_short | Bone Protective Effect of Extra-Virgin Olive Oil Phenolic Compounds by Modulating Osteoblast Gene Expression |
| title_sort | bone protective effect of extra virgin olive oil phenolic compounds by modulating osteoblast gene expression |
| topic | extra virgin olive oil phenolic compounds gene expression osteoblast genetic profile |
| url | https://www.mdpi.com/2072-6643/11/8/1722 |
| work_keys_str_mv | AT luciamelguizorodriguez boneprotectiveeffectofextravirginoliveoilphenoliccompoundsbymodulatingosteoblastgeneexpression AT franciscojaviermanzanomoreno boneprotectiveeffectofextravirginoliveoilphenoliccompoundsbymodulatingosteoblastgeneexpression AT rebecaillescasmontes boneprotectiveeffectofextravirginoliveoilphenoliccompoundsbymodulatingosteoblastgeneexpression AT javierramostorrecillas boneprotectiveeffectofextravirginoliveoilphenoliccompoundsbymodulatingosteoblastgeneexpression AT elviradelunabertos boneprotectiveeffectofextravirginoliveoilphenoliccompoundsbymodulatingosteoblastgeneexpression AT concepcionruiz boneprotectiveeffectofextravirginoliveoilphenoliccompoundsbymodulatingosteoblastgeneexpression AT olgagarciamartinez boneprotectiveeffectofextravirginoliveoilphenoliccompoundsbymodulatingosteoblastgeneexpression |