A protocol to characterize zebrafish LGP2 as a dual regulator of IFN response during viral infection
Summary: Here, we present a protocol to characterize zebrafish LGP2 as a dual regulator of interferon (IFN) response. We detail in vivo assays using time-lapse comparison of IFN response between wild-type and lgp2 knockout zebrafish following spring viraemia of carp virus (SVCV) infection. We also d...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-12-01
|
| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166722007249 |
| _version_ | 1811199365008588800 |
|---|---|
| author | Xiu-Ying Gong Yi-Bing Zhang |
| author_facet | Xiu-Ying Gong Yi-Bing Zhang |
| author_sort | Xiu-Ying Gong |
| collection | DOAJ |
| description | Summary: Here, we present a protocol to characterize zebrafish LGP2 as a dual regulator of interferon (IFN) response. We detail in vivo assays using time-lapse comparison of IFN response between wild-type and lgp2 knockout zebrafish following spring viraemia of carp virus (SVCV) infection. We also describe in vitro assays including titration of infection duration in SVCV-infected fish cells to determine changes in IFN response. This protocol is effective to illuminate a regulatory switch of LGP2 in fish cells toward virus infection.For complete details on the use and execution of this protocol, please refer to Gong et al. (2022).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-04-12T01:46:59Z |
| format | Article |
| id | doaj.art-2ca83f90b99945f89ea76eaa9acb191f |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-04-12T01:46:59Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-2ca83f90b99945f89ea76eaa9acb191f2022-12-22T03:53:02ZengElsevierSTAR Protocols2666-16672022-12-0134101844A protocol to characterize zebrafish LGP2 as a dual regulator of IFN response during viral infectionXiu-Ying Gong0Yi-Bing Zhang1State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; University of Chinese Academy of Sciences, Beijing 10049, ChinaState Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; University of Chinese Academy of Sciences, Beijing 10049, China; The Innovation Academy of Seed Design, Chinese Academy of Sciences, Wuhan 430072, China; Key Laboratory of Aquaculture Disease Control of Ministry of Agriculture, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; Corresponding authorSummary: Here, we present a protocol to characterize zebrafish LGP2 as a dual regulator of interferon (IFN) response. We detail in vivo assays using time-lapse comparison of IFN response between wild-type and lgp2 knockout zebrafish following spring viraemia of carp virus (SVCV) infection. We also describe in vitro assays including titration of infection duration in SVCV-infected fish cells to determine changes in IFN response. This protocol is effective to illuminate a regulatory switch of LGP2 in fish cells toward virus infection.For complete details on the use and execution of this protocol, please refer to Gong et al. (2022).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166722007249ImmunologyModel OrganismsMolecular BiologySignal TransductionProtein Biochemistry |
| spellingShingle | Xiu-Ying Gong Yi-Bing Zhang A protocol to characterize zebrafish LGP2 as a dual regulator of IFN response during viral infection STAR Protocols Immunology Model Organisms Molecular Biology Signal Transduction Protein Biochemistry |
| title | A protocol to characterize zebrafish LGP2 as a dual regulator of IFN response during viral infection |
| title_full | A protocol to characterize zebrafish LGP2 as a dual regulator of IFN response during viral infection |
| title_fullStr | A protocol to characterize zebrafish LGP2 as a dual regulator of IFN response during viral infection |
| title_full_unstemmed | A protocol to characterize zebrafish LGP2 as a dual regulator of IFN response during viral infection |
| title_short | A protocol to characterize zebrafish LGP2 as a dual regulator of IFN response during viral infection |
| title_sort | protocol to characterize zebrafish lgp2 as a dual regulator of ifn response during viral infection |
| topic | Immunology Model Organisms Molecular Biology Signal Transduction Protein Biochemistry |
| url | http://www.sciencedirect.com/science/article/pii/S2666166722007249 |
| work_keys_str_mv | AT xiuyinggong aprotocoltocharacterizezebrafishlgp2asadualregulatorofifnresponseduringviralinfection AT yibingzhang aprotocoltocharacterizezebrafishlgp2asadualregulatorofifnresponseduringviralinfection AT xiuyinggong protocoltocharacterizezebrafishlgp2asadualregulatorofifnresponseduringviralinfection AT yibingzhang protocoltocharacterizezebrafishlgp2asadualregulatorofifnresponseduringviralinfection |