Circovirus in Blood of a Febrile Horse with Hepatitis
Circoviruses infect vertebrates where they can result in a wide range of disease signs or in asymptomatic infections. Using viral metagenomics we analyzed a pool of five sera from four healthy and one sick horse. Sequences from parvovirus-H, equus anellovirus, and distantly related to mammalian circ...
| मुख्य लेखकों: | , , , , , |
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| स्वरूप: | लेख |
| भाषा: | English |
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MDPI AG
2021-05-01
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| श्रृंखला: | Viruses |
| विषय: | |
| ऑनलाइन पहुंच: | https://www.mdpi.com/1999-4915/13/5/944 |
| _version_ | 1827691973735088128 |
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| author | Alvin Hui Eda Altan Nathan Slovis Caitlin Fletcher Xutao Deng Eric Delwart |
| author_facet | Alvin Hui Eda Altan Nathan Slovis Caitlin Fletcher Xutao Deng Eric Delwart |
| author_sort | Alvin Hui |
| collection | DOAJ |
| description | Circoviruses infect vertebrates where they can result in a wide range of disease signs or in asymptomatic infections. Using viral metagenomics we analyzed a pool of five sera from four healthy and one sick horse. Sequences from parvovirus-H, equus anellovirus, and distantly related to mammalian circoviruses were recognized. PCR identified the circovirus reads as originating from a pregnant mare with fever and hepatitis. That horse’s serum was also positive by real time PCR for equine parvovirus H and negative for the flavivirus equine hepacivirus. The complete circular genome of equine circovirus 1 strain Charaf (EqCV1-Charaf) was completed using PCR and Sanger sequencing. EqCV1 replicase showed 73–74% identity to those of their closest relatives, pig circoviruses 1/2, and elk circovirus. The closest capsid proteins were from the same ungulate circoviruses with 62–63% identity. The overall nucleotide identity of 72% to its closest relative indicates that EqCV1 is a new species in the <i>Circovirus</i> genus, the first reported in genus Equus. Whether EqCV1 alone or in co-infections can result in disease and its prevalence in different equine populations will require further studies now facilitated using EqCV1′s genome sequence. |
| first_indexed | 2024-03-10T11:13:00Z |
| format | Article |
| id | doaj.art-2ca8a3e3d3e64a0190ceb64a21597b1c |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-10T11:13:00Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-2ca8a3e3d3e64a0190ceb64a21597b1c2023-11-21T20:38:02ZengMDPI AGViruses1999-49152021-05-0113594410.3390/v13050944Circovirus in Blood of a Febrile Horse with HepatitisAlvin Hui0Eda Altan1Nathan Slovis2Caitlin Fletcher3Xutao Deng4Eric Delwart5Vitalant Research Institute, San Francisco, CA 94118, USAVitalant Research Institute, San Francisco, CA 94118, USAHagyard Equine Medical Institute, 4250 Iron Works Pike, Lexington, KY 40361, USAHagyard Equine Medical Institute, 4250 Iron Works Pike, Lexington, KY 40361, USAVitalant Research Institute, San Francisco, CA 94118, USAVitalant Research Institute, San Francisco, CA 94118, USACircoviruses infect vertebrates where they can result in a wide range of disease signs or in asymptomatic infections. Using viral metagenomics we analyzed a pool of five sera from four healthy and one sick horse. Sequences from parvovirus-H, equus anellovirus, and distantly related to mammalian circoviruses were recognized. PCR identified the circovirus reads as originating from a pregnant mare with fever and hepatitis. That horse’s serum was also positive by real time PCR for equine parvovirus H and negative for the flavivirus equine hepacivirus. The complete circular genome of equine circovirus 1 strain Charaf (EqCV1-Charaf) was completed using PCR and Sanger sequencing. EqCV1 replicase showed 73–74% identity to those of their closest relatives, pig circoviruses 1/2, and elk circovirus. The closest capsid proteins were from the same ungulate circoviruses with 62–63% identity. The overall nucleotide identity of 72% to its closest relative indicates that EqCV1 is a new species in the <i>Circovirus</i> genus, the first reported in genus Equus. Whether EqCV1 alone or in co-infections can result in disease and its prevalence in different equine populations will require further studies now facilitated using EqCV1′s genome sequence.https://www.mdpi.com/1999-4915/13/5/944<i>Equues caballus</i><i>Circoviridae</i>viral metagenomicshepatitis |
| spellingShingle | Alvin Hui Eda Altan Nathan Slovis Caitlin Fletcher Xutao Deng Eric Delwart Circovirus in Blood of a Febrile Horse with Hepatitis Viruses <i>Equues caballus</i> <i>Circoviridae</i> viral metagenomics hepatitis |
| title | Circovirus in Blood of a Febrile Horse with Hepatitis |
| title_full | Circovirus in Blood of a Febrile Horse with Hepatitis |
| title_fullStr | Circovirus in Blood of a Febrile Horse with Hepatitis |
| title_full_unstemmed | Circovirus in Blood of a Febrile Horse with Hepatitis |
| title_short | Circovirus in Blood of a Febrile Horse with Hepatitis |
| title_sort | circovirus in blood of a febrile horse with hepatitis |
| topic | <i>Equues caballus</i> <i>Circoviridae</i> viral metagenomics hepatitis |
| url | https://www.mdpi.com/1999-4915/13/5/944 |
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