Antifungal activity of microbial nanoparticles derived from Chaetomium spp. against Magnaporthe oryzae causing rice blast
The Magnaporthe oryzae isolate PO2 was proven to cause rice blast var. PSL 2 in this study. Chaetomium elatum, Chaetomium lucknowense and Chaetomium brasiliense were antagonised to M. oryzae. The hexane extract of C. brasiliense gave the highest inhibition of the spore production with an ED50 of 35...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2020-09-01
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| Series: | Plant Protection Science |
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| Online Access: | https://pps.agriculturejournals.cz/artkey/pps-202003-0004_antifungal-activity-of-microbial-nanoparticles-derived-from-chaetomium-spp-against-magnaporthe-oryzae-causing.php |
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| author | Jiao Jiao Song Kasem Soytong Somdej Kanokmedhakul Kwanjai Kanokmedhakul Supattra Poeaim |
| author_facet | Jiao Jiao Song Kasem Soytong Somdej Kanokmedhakul Kwanjai Kanokmedhakul Supattra Poeaim |
| author_sort | Jiao Jiao Song |
| collection | DOAJ |
| description | The Magnaporthe oryzae isolate PO2 was proven to cause rice blast var. PSL 2 in this study. Chaetomium elatum, Chaetomium lucknowense and Chaetomium brasiliense were antagonised to M. oryzae. The hexane extract of C. brasiliense gave the highest inhibition of the spore production with an ED50 of 35 ppm, the EtOAC extract of C. lucknowense inhibited the spore production at 57 ppm and the EtOAC extracts of C. elatum inhibited the spore production at 106 ppm. The nano-CLM (C. lucknowense) inhibited the spore production at 5.24 ppm, the nano-CBH (C. brasiliense) inhibited the spore production at 6.86 ppm and the nano-CEE (C. elatum) inhibited the spore production at 7.89 ppm. The rice leaves treated with nano-CBH from C. brasiliense produced Sakuranertin and Oryzalexin B as seen on the thin layer chromatography where the Rf value was 0.08 assumed to be Sakuranertin, and the Rf value of 0.28 supposed to be Oryzalexin B. It was found that the nanoparticles act as elicitors to induce immunity in rice plants through the production of phytoalexin. |
| first_indexed | 2024-04-10T08:13:50Z |
| format | Article |
| id | doaj.art-2ca8d4ce57c942298052be5a7012b432 |
| institution | Directory Open Access Journal |
| issn | 1212-2580 1805-9341 |
| language | English |
| last_indexed | 2024-04-10T08:13:50Z |
| publishDate | 2020-09-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Plant Protection Science |
| spelling | doaj.art-2ca8d4ce57c942298052be5a7012b4322023-02-23T03:44:30ZengCzech Academy of Agricultural SciencesPlant Protection Science1212-25801805-93412020-09-0156318019010.17221/41/2019-PPSpps-202003-0004Antifungal activity of microbial nanoparticles derived from Chaetomium spp. against Magnaporthe oryzae causing rice blastJiao Jiao Song0Kasem Soytong1Somdej Kanokmedhakul2Kwanjai Kanokmedhakul3Supattra Poeaim4Department of Plant Production Technology, Faculty of Agricultural Technology, King Mongkut's Institute of Technology Ladkrabang, Ladkrabang, Bangkok, ThailandDepartment of Plant Production Technology, Faculty of Agricultural Technology, King Mongkut's Institute of Technology Ladkrabang, Ladkrabang, Bangkok, ThailandNatural Products Research Unit, Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Khon Kaen University, Khon Kaen, ThailandNatural Products Research Unit, Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Khon Kaen University, Khon Kaen, ThailandDepartment of Biology, Faculty of Science, King Mongkut's Institute of Technology Ladkrabang, Ladkrabang, Bangkok, ThailandThe Magnaporthe oryzae isolate PO2 was proven to cause rice blast var. PSL 2 in this study. Chaetomium elatum, Chaetomium lucknowense and Chaetomium brasiliense were antagonised to M. oryzae. The hexane extract of C. brasiliense gave the highest inhibition of the spore production with an ED50 of 35 ppm, the EtOAC extract of C. lucknowense inhibited the spore production at 57 ppm and the EtOAC extracts of C. elatum inhibited the spore production at 106 ppm. The nano-CLM (C. lucknowense) inhibited the spore production at 5.24 ppm, the nano-CBH (C. brasiliense) inhibited the spore production at 6.86 ppm and the nano-CEE (C. elatum) inhibited the spore production at 7.89 ppm. The rice leaves treated with nano-CBH from C. brasiliense produced Sakuranertin and Oryzalexin B as seen on the thin layer chromatography where the Rf value was 0.08 assumed to be Sakuranertin, and the Rf value of 0.28 supposed to be Oryzalexin B. It was found that the nanoparticles act as elicitors to induce immunity in rice plants through the production of phytoalexin.https://pps.agriculturejournals.cz/artkey/pps-202003-0004_antifungal-activity-of-microbial-nanoparticles-derived-from-chaetomium-spp-against-magnaporthe-oryzae-causing.phpplant pathologybiological controlantagonistic fungiblast disease |
| spellingShingle | Jiao Jiao Song Kasem Soytong Somdej Kanokmedhakul Kwanjai Kanokmedhakul Supattra Poeaim Antifungal activity of microbial nanoparticles derived from Chaetomium spp. against Magnaporthe oryzae causing rice blast Plant Protection Science plant pathology biological control antagonistic fungi blast disease |
| title | Antifungal activity of microbial nanoparticles derived from Chaetomium spp. against Magnaporthe oryzae causing rice blast |
| title_full | Antifungal activity of microbial nanoparticles derived from Chaetomium spp. against Magnaporthe oryzae causing rice blast |
| title_fullStr | Antifungal activity of microbial nanoparticles derived from Chaetomium spp. against Magnaporthe oryzae causing rice blast |
| title_full_unstemmed | Antifungal activity of microbial nanoparticles derived from Chaetomium spp. against Magnaporthe oryzae causing rice blast |
| title_short | Antifungal activity of microbial nanoparticles derived from Chaetomium spp. against Magnaporthe oryzae causing rice blast |
| title_sort | antifungal activity of microbial nanoparticles derived from chaetomium spp against magnaporthe oryzae causing rice blast |
| topic | plant pathology biological control antagonistic fungi blast disease |
| url | https://pps.agriculturejournals.cz/artkey/pps-202003-0004_antifungal-activity-of-microbial-nanoparticles-derived-from-chaetomium-spp-against-magnaporthe-oryzae-causing.php |
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