A cassava common mosaic virus vector for virus-induced gene silencing in cassava
Abstract Background Cassava is an important crop for food security and industry in the least-developed and developing countries. The completion of the cassava genome sequence and identification of large numbers of candidate genes by next-generation sequencing provide extensive resources for cassava...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2021-07-01
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| Series: | Plant Methods |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13007-021-00775-w |
| _version_ | 1818624250266779648 |
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| author | Decai Tuo Peng Zhou Pu Yan Hongguang Cui Yang Liu He Wang Xiukun Yang Wenbin Liao Di Sun Xiaoying Li Wentao Shen |
| author_facet | Decai Tuo Peng Zhou Pu Yan Hongguang Cui Yang Liu He Wang Xiukun Yang Wenbin Liao Di Sun Xiaoying Li Wentao Shen |
| author_sort | Decai Tuo |
| collection | DOAJ |
| description | Abstract Background Cassava is an important crop for food security and industry in the least-developed and developing countries. The completion of the cassava genome sequence and identification of large numbers of candidate genes by next-generation sequencing provide extensive resources for cassava molecular breeding and increase the need for rapid and efficient gene function analysis systems in cassava. Several plant virus-induced gene silencing (VIGS) systems have been developed as reverse genetic tools for rapid gene function analysis in cassava. However, these VIGS vectors could cause severe viral symptoms or inefficient gene silencing. Results In this study, we constructed agroinfection-compatible infectious cDNA clones of cassava common mosaic virus isolate CM (CsCMV-CM, genus Potexvirus, family Alphaflexiviridae) that causes systemic infection with mild symptoms in cassava. CsCMV-CM was then modified to a viral vector carrying the Nimble cloning frame, which facilitates the rapid and high-throughput cloning of silencing fragments into the viral genome. The CsCMV-based vector successfully silenced phytoene desaturase (PDS) and magnesium chelatase subunit I (ChlI) in different cassava varieties and Nicotiana benthamiana. The silencing of the ChlI gene could persist for more than two months. Conclusions This CsCMV-based VIGS system provides a new tool for rapid and efficient gene function studies in cassava. |
| first_indexed | 2024-12-16T18:53:58Z |
| format | Article |
| id | doaj.art-2caa61c921774d0ea643fd45ff3a99c6 |
| institution | Directory Open Access Journal |
| issn | 1746-4811 |
| language | English |
| last_indexed | 2024-12-16T18:53:58Z |
| publishDate | 2021-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Plant Methods |
| spelling | doaj.art-2caa61c921774d0ea643fd45ff3a99c62022-12-21T22:20:36ZengBMCPlant Methods1746-48112021-07-0117111210.1186/s13007-021-00775-wA cassava common mosaic virus vector for virus-induced gene silencing in cassavaDecai Tuo0Peng Zhou1Pu Yan2Hongguang Cui3Yang Liu4He Wang5Xiukun Yang6Wenbin Liao7Di Sun8Xiaoying Li9Wentao Shen10Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture and Rural Affairs & Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural SciencesKey Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture and Rural Affairs & Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural SciencesKey Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture and Rural Affairs & Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural SciencesCollege of Plant Protection, Hainan UniversityKey Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture and Rural Affairs & Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural SciencesKey Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture and Rural Affairs & Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural SciencesKey Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture and Rural Affairs & Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural SciencesKey Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture and Rural Affairs & Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural SciencesKey Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture and Rural Affairs & Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural SciencesKey Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture and Rural Affairs & Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural SciencesKey Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture and Rural Affairs & Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural SciencesAbstract Background Cassava is an important crop for food security and industry in the least-developed and developing countries. The completion of the cassava genome sequence and identification of large numbers of candidate genes by next-generation sequencing provide extensive resources for cassava molecular breeding and increase the need for rapid and efficient gene function analysis systems in cassava. Several plant virus-induced gene silencing (VIGS) systems have been developed as reverse genetic tools for rapid gene function analysis in cassava. However, these VIGS vectors could cause severe viral symptoms or inefficient gene silencing. Results In this study, we constructed agroinfection-compatible infectious cDNA clones of cassava common mosaic virus isolate CM (CsCMV-CM, genus Potexvirus, family Alphaflexiviridae) that causes systemic infection with mild symptoms in cassava. CsCMV-CM was then modified to a viral vector carrying the Nimble cloning frame, which facilitates the rapid and high-throughput cloning of silencing fragments into the viral genome. The CsCMV-based vector successfully silenced phytoene desaturase (PDS) and magnesium chelatase subunit I (ChlI) in different cassava varieties and Nicotiana benthamiana. The silencing of the ChlI gene could persist for more than two months. Conclusions This CsCMV-based VIGS system provides a new tool for rapid and efficient gene function studies in cassava.https://doi.org/10.1186/s13007-021-00775-wCassavaCassava common mosaic virusVirus vectorVirus-induced gene silencing |
| spellingShingle | Decai Tuo Peng Zhou Pu Yan Hongguang Cui Yang Liu He Wang Xiukun Yang Wenbin Liao Di Sun Xiaoying Li Wentao Shen A cassava common mosaic virus vector for virus-induced gene silencing in cassava Plant Methods Cassava Cassava common mosaic virus Virus vector Virus-induced gene silencing |
| title | A cassava common mosaic virus vector for virus-induced gene silencing in cassava |
| title_full | A cassava common mosaic virus vector for virus-induced gene silencing in cassava |
| title_fullStr | A cassava common mosaic virus vector for virus-induced gene silencing in cassava |
| title_full_unstemmed | A cassava common mosaic virus vector for virus-induced gene silencing in cassava |
| title_short | A cassava common mosaic virus vector for virus-induced gene silencing in cassava |
| title_sort | cassava common mosaic virus vector for virus induced gene silencing in cassava |
| topic | Cassava Cassava common mosaic virus Virus vector Virus-induced gene silencing |
| url | https://doi.org/10.1186/s13007-021-00775-w |
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