Geniposidic Acid from <i>Eucommia ulmoides</i> Oliver Staminate Flower Tea Mitigates Cellular Oxidative Stress via Activating AKT/NRF2 Signaling
<i>Eucommia ulmoides</i> Oliver staminate flower (ESF) tea enjoys a good reputation in folk medicine and displays multiple bioactivities, such as antioxidant and antifatigue properties. However, the underlying biological mechanisms remain largely unknown. In this study, we aimed to inves...
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MDPI AG
2022-12-01
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| Series: | Molecules |
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| author | Shuo Cheng Huiling Jia Yisen Zhang Juanjuan Zhou Xue Chen Lifang Wu Jun Wang |
| author_facet | Shuo Cheng Huiling Jia Yisen Zhang Juanjuan Zhou Xue Chen Lifang Wu Jun Wang |
| author_sort | Shuo Cheng |
| collection | DOAJ |
| description | <i>Eucommia ulmoides</i> Oliver staminate flower (ESF) tea enjoys a good reputation in folk medicine and displays multiple bioactivities, such as antioxidant and antifatigue properties. However, the underlying biological mechanisms remain largely unknown. In this study, we aimed to investigate whether ESF tea can mitigate cellular oxidative stress. Crude ethyl alcohol extract and its three subfractions prepared by sequential extraction with chloroform, n-butyl alcohol and residual water were prepared from ESF tea. The results of antioxidant activity tests in vitro manifested n-butyl alcohol fraction (n-BUF) showed the strongest antioxidant capacity (DPPH: IC<sub>50</sub> = 24.45 ± 0.74 μg/mL, ABTS: IC<sub>50</sub> = 17.25 ± 0.04 μg/mL). Moreover, all subfractions of ESF tea, especially the n-BUF, exhibited an obvious capacity to scavenge the reactive oxygen species (ROS) and stimulate the NRF2 antioxidative response in human keratinocytes HaCaT treated by H<sub>2</sub>O<sub>2</sub>. Using ultra-high-performance liquid chromatography, we identified geniposidic acid (GPA) as the most abundant component in ESF tea extract. Furthermore, it was found that GPA relieved oxidative stress in H<sub>2</sub>O<sub>2</sub>-induced HaCaT cells by activating the Akt/Nrf2/OGG1 pathway. Our findings indicated that ESF tea may be a source of natural antioxidants to protect against skin cell oxidative damage and deserves further development and utilization. |
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| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T17:38:28Z |
| publishDate | 2022-12-01 |
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| series | Molecules |
| spelling | doaj.art-2cb3b94bb9534b548d5b12a98c5776ab2023-11-24T11:44:29ZengMDPI AGMolecules1420-30492022-12-012723856810.3390/molecules27238568Geniposidic Acid from <i>Eucommia ulmoides</i> Oliver Staminate Flower Tea Mitigates Cellular Oxidative Stress via Activating AKT/NRF2 SignalingShuo Cheng0Huiling Jia1Yisen Zhang2Juanjuan Zhou3Xue Chen4Lifang Wu5Jun Wang6The Center for Ion Beam Bioengineering & Green Agriculture, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, ChinaThe Center for Ion Beam Bioengineering & Green Agriculture, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, ChinaThe Center for Ion Beam Bioengineering & Green Agriculture, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, ChinaThe Center for Ion Beam Bioengineering & Green Agriculture, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, ChinaThe Center for Ion Beam Bioengineering & Green Agriculture, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, ChinaThe Center for Ion Beam Bioengineering & Green Agriculture, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, ChinaThe Center for Ion Beam Bioengineering & Green Agriculture, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, China<i>Eucommia ulmoides</i> Oliver staminate flower (ESF) tea enjoys a good reputation in folk medicine and displays multiple bioactivities, such as antioxidant and antifatigue properties. However, the underlying biological mechanisms remain largely unknown. In this study, we aimed to investigate whether ESF tea can mitigate cellular oxidative stress. Crude ethyl alcohol extract and its three subfractions prepared by sequential extraction with chloroform, n-butyl alcohol and residual water were prepared from ESF tea. The results of antioxidant activity tests in vitro manifested n-butyl alcohol fraction (n-BUF) showed the strongest antioxidant capacity (DPPH: IC<sub>50</sub> = 24.45 ± 0.74 μg/mL, ABTS: IC<sub>50</sub> = 17.25 ± 0.04 μg/mL). Moreover, all subfractions of ESF tea, especially the n-BUF, exhibited an obvious capacity to scavenge the reactive oxygen species (ROS) and stimulate the NRF2 antioxidative response in human keratinocytes HaCaT treated by H<sub>2</sub>O<sub>2</sub>. Using ultra-high-performance liquid chromatography, we identified geniposidic acid (GPA) as the most abundant component in ESF tea extract. Furthermore, it was found that GPA relieved oxidative stress in H<sub>2</sub>O<sub>2</sub>-induced HaCaT cells by activating the Akt/Nrf2/OGG1 pathway. Our findings indicated that ESF tea may be a source of natural antioxidants to protect against skin cell oxidative damage and deserves further development and utilization.https://www.mdpi.com/1420-3049/27/23/8568<i>Eucommia ulmoides</i> Oliver staminate flower teaoxidative stressAKTNRF2geniposidic acidkeratinocyte |
| spellingShingle | Shuo Cheng Huiling Jia Yisen Zhang Juanjuan Zhou Xue Chen Lifang Wu Jun Wang Geniposidic Acid from <i>Eucommia ulmoides</i> Oliver Staminate Flower Tea Mitigates Cellular Oxidative Stress via Activating AKT/NRF2 Signaling Molecules <i>Eucommia ulmoides</i> Oliver staminate flower tea oxidative stress AKT NRF2 geniposidic acid keratinocyte |
| title | Geniposidic Acid from <i>Eucommia ulmoides</i> Oliver Staminate Flower Tea Mitigates Cellular Oxidative Stress via Activating AKT/NRF2 Signaling |
| title_full | Geniposidic Acid from <i>Eucommia ulmoides</i> Oliver Staminate Flower Tea Mitigates Cellular Oxidative Stress via Activating AKT/NRF2 Signaling |
| title_fullStr | Geniposidic Acid from <i>Eucommia ulmoides</i> Oliver Staminate Flower Tea Mitigates Cellular Oxidative Stress via Activating AKT/NRF2 Signaling |
| title_full_unstemmed | Geniposidic Acid from <i>Eucommia ulmoides</i> Oliver Staminate Flower Tea Mitigates Cellular Oxidative Stress via Activating AKT/NRF2 Signaling |
| title_short | Geniposidic Acid from <i>Eucommia ulmoides</i> Oliver Staminate Flower Tea Mitigates Cellular Oxidative Stress via Activating AKT/NRF2 Signaling |
| title_sort | geniposidic acid from i eucommia ulmoides i oliver staminate flower tea mitigates cellular oxidative stress via activating akt nrf2 signaling |
| topic | <i>Eucommia ulmoides</i> Oliver staminate flower tea oxidative stress AKT NRF2 geniposidic acid keratinocyte |
| url | https://www.mdpi.com/1420-3049/27/23/8568 |
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