(Hydroxypropyl)methyl Cellulose-Chitosan Film as a Matrix for Lipase Immobilization—Part ΙΙ: Structural Studies
The present work reports on the structural study of a film made of a hybrid blend of biopolymers used as an enzyme carrier. A cellulose derivative (HPMC) and chitosan (CS) were combined in order to formulate a film on which <i>Mucor miehei</i> lipase was immobilized. The film was success...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-09-01
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| Online Access: | https://www.mdpi.com/2310-2861/8/9/595 |
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| author | Evdokia Vassiliadi Marta Tsirigotis-Maniecka Henry E. Symons Pierangelo Gobbo Frédéric Nallet Aristotelis Xenakis Maria Zoumpanioti |
| author_facet | Evdokia Vassiliadi Marta Tsirigotis-Maniecka Henry E. Symons Pierangelo Gobbo Frédéric Nallet Aristotelis Xenakis Maria Zoumpanioti |
| author_sort | Evdokia Vassiliadi |
| collection | DOAJ |
| description | The present work reports on the structural study of a film made of a hybrid blend of biopolymers used as an enzyme carrier. A cellulose derivative (HPMC) and chitosan (CS) were combined in order to formulate a film on which <i>Mucor miehei</i> lipase was immobilized. The film was successfully used as a biocatalyst; however, little is known about the structure of the system. Therefore, small-angle X-ray scattering, Fourier transform infrared spectroscopy (FTIR), optical microscopy, and scanning electron microscopy (SEM), as well as microindentation measurements, were used to shed light on the structure of the promising biocatalyst. Among the results, intermolecular hydrogen bonds were observed between the amide groups of the two polymers and the lipase. The presence of the enzyme does not seem to affect the mechanical properties of the matrix. The used film after 35 cycles of reaction seemed to be fatigued and had lost part of its humidity, explaining the reduction of the enzyme activity. |
| first_indexed | 2024-03-09T23:57:12Z |
| format | Article |
| id | doaj.art-2cb953219f0e4ab1bafb01046b6131f5 |
| institution | Directory Open Access Journal |
| issn | 2310-2861 |
| language | English |
| last_indexed | 2024-03-09T23:57:12Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Gels |
| spelling | doaj.art-2cb953219f0e4ab1bafb01046b6131f52023-11-23T16:22:42ZengMDPI AGGels2310-28612022-09-018959510.3390/gels8090595(Hydroxypropyl)methyl Cellulose-Chitosan Film as a Matrix for Lipase Immobilization—Part ΙΙ: Structural StudiesEvdokia Vassiliadi0Marta Tsirigotis-Maniecka1Henry E. Symons2Pierangelo Gobbo3Frédéric Nallet4Aristotelis Xenakis5Maria Zoumpanioti6Institute of Chemical Biology, National Hellenic Research Foundation (NHRF), 48 Vassileos Constantinou Ave., 11635 Athens, GreeceFaculty of Chemistry, Wrocław University of Science and Technology, 50-370 Wroclaw, PolandSchool of Chemistry, University of Bristol, Bristol BS8 1TS, UKDepartment of Chemical and Pharmaceutical Sciences, University of Trieste, 34127 Trieste, ItalyCentre de Recherche Paul-Pascal, University Bordeaux, CNRS, UMR 5031, 115 Avenue du Docteur-Schweitzer, 33600 Pessac, FranceInstitute of Chemical Biology, National Hellenic Research Foundation (NHRF), 48 Vassileos Constantinou Ave., 11635 Athens, GreeceInstitute of Chemical Biology, National Hellenic Research Foundation (NHRF), 48 Vassileos Constantinou Ave., 11635 Athens, GreeceThe present work reports on the structural study of a film made of a hybrid blend of biopolymers used as an enzyme carrier. A cellulose derivative (HPMC) and chitosan (CS) were combined in order to formulate a film on which <i>Mucor miehei</i> lipase was immobilized. The film was successfully used as a biocatalyst; however, little is known about the structure of the system. Therefore, small-angle X-ray scattering, Fourier transform infrared spectroscopy (FTIR), optical microscopy, and scanning electron microscopy (SEM), as well as microindentation measurements, were used to shed light on the structure of the promising biocatalyst. Among the results, intermolecular hydrogen bonds were observed between the amide groups of the two polymers and the lipase. The presence of the enzyme does not seem to affect the mechanical properties of the matrix. The used film after 35 cycles of reaction seemed to be fatigued and had lost part of its humidity, explaining the reduction of the enzyme activity.https://www.mdpi.com/2310-2861/8/9/595hydroxypropylmethyl cellulosecharacterizationblended polymersfilmenzyme immobilizationlipase |
| spellingShingle | Evdokia Vassiliadi Marta Tsirigotis-Maniecka Henry E. Symons Pierangelo Gobbo Frédéric Nallet Aristotelis Xenakis Maria Zoumpanioti (Hydroxypropyl)methyl Cellulose-Chitosan Film as a Matrix for Lipase Immobilization—Part ΙΙ: Structural Studies Gels hydroxypropylmethyl cellulose characterization blended polymers film enzyme immobilization lipase |
| title | (Hydroxypropyl)methyl Cellulose-Chitosan Film as a Matrix for Lipase Immobilization—Part ΙΙ: Structural Studies |
| title_full | (Hydroxypropyl)methyl Cellulose-Chitosan Film as a Matrix for Lipase Immobilization—Part ΙΙ: Structural Studies |
| title_fullStr | (Hydroxypropyl)methyl Cellulose-Chitosan Film as a Matrix for Lipase Immobilization—Part ΙΙ: Structural Studies |
| title_full_unstemmed | (Hydroxypropyl)methyl Cellulose-Chitosan Film as a Matrix for Lipase Immobilization—Part ΙΙ: Structural Studies |
| title_short | (Hydroxypropyl)methyl Cellulose-Chitosan Film as a Matrix for Lipase Immobilization—Part ΙΙ: Structural Studies |
| title_sort | hydroxypropyl methyl cellulose chitosan film as a matrix for lipase immobilization part ιι structural studies |
| topic | hydroxypropylmethyl cellulose characterization blended polymers film enzyme immobilization lipase |
| url | https://www.mdpi.com/2310-2861/8/9/595 |
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