Snapshot of methanogen sensitivity to temperature in Zoige wetland from Tibetan plateau
Zoige wetland in Tibetan plateau represents a cold environment at high altitude where significant methane emission has been observed. However, it remains unknown how the production and emission of CH4 from Zoige wetland will respond to a warming climate. Here we investigated the temperature sensitiv...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2015-02-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.00131/full |
| _version_ | 1811213474710159360 |
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| author | Li eFu Tianze eSong Yahai eLu Yahai eLu |
| author_facet | Li eFu Tianze eSong Yahai eLu Yahai eLu |
| author_sort | Li eFu |
| collection | DOAJ |
| description | Zoige wetland in Tibetan plateau represents a cold environment at high altitude where significant methane emission has been observed. However, it remains unknown how the production and emission of CH4 from Zoige wetland will respond to a warming climate. Here we investigated the temperature sensitivity of methanogen community in a Zoige wetland soil under the laboratory incubation conditions. One soil sample was collected and the temperature sensitivity of the methanogenic activity, the structure of methanogen community and the methanogenic pathways were determined. We found that the response of methanogenesis to temperature could be separated into two phases, a high sensitivity in the low temperature range and a modest sensitivity under mesophilic conditions, respectively. The aceticlastic methanogens Methanosarcinaceae were the main methanogens at low temperatures, while hydrogenotrophic Methanobacteriales, Methanomicrobiales and Methanocellales were more abundant at higher temperatures. The total abundance of mcrA genes increased with temperature indicating that the growth of methanogens was stimulated. The growth of hydrogenotrophic methanogens, however, was faster than aceticlastic ones resulting in the shift of methanogen community. Determination of carbon isotopic signatures indicated that methanogenic pathway was also shifted from mainly aceticlastic methanogenesis to a mixture of hydrogenotrophic and aceticlastic methanogenesis with the increase of temperature. Collectively, the shift of temperature responses of methanogenesis was in accordance with the changes in methanogen composition and methanogenic pathway in this Zoige wetland sample. It appears that the aceticlastic methanogenesis dominated at low temperatures is more sensitive than the hydrogenotrophic one at higher temperatures. |
| first_indexed | 2024-04-12T05:47:21Z |
| format | Article |
| id | doaj.art-2cb99ecf34dd4c90a4b12f595f4461aa |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-12T05:47:21Z |
| publishDate | 2015-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-2cb99ecf34dd4c90a4b12f595f4461aa2022-12-22T03:45:24ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2015-02-01610.3389/fmicb.2015.00131125302Snapshot of methanogen sensitivity to temperature in Zoige wetland from Tibetan plateauLi eFu0Tianze eSong1Yahai eLu2Yahai eLu3China Agriculture UniversityFudan UniversityChina Agriculture UniversityPeking UniversityZoige wetland in Tibetan plateau represents a cold environment at high altitude where significant methane emission has been observed. However, it remains unknown how the production and emission of CH4 from Zoige wetland will respond to a warming climate. Here we investigated the temperature sensitivity of methanogen community in a Zoige wetland soil under the laboratory incubation conditions. One soil sample was collected and the temperature sensitivity of the methanogenic activity, the structure of methanogen community and the methanogenic pathways were determined. We found that the response of methanogenesis to temperature could be separated into two phases, a high sensitivity in the low temperature range and a modest sensitivity under mesophilic conditions, respectively. The aceticlastic methanogens Methanosarcinaceae were the main methanogens at low temperatures, while hydrogenotrophic Methanobacteriales, Methanomicrobiales and Methanocellales were more abundant at higher temperatures. The total abundance of mcrA genes increased with temperature indicating that the growth of methanogens was stimulated. The growth of hydrogenotrophic methanogens, however, was faster than aceticlastic ones resulting in the shift of methanogen community. Determination of carbon isotopic signatures indicated that methanogenic pathway was also shifted from mainly aceticlastic methanogenesis to a mixture of hydrogenotrophic and aceticlastic methanogenesis with the increase of temperature. Collectively, the shift of temperature responses of methanogenesis was in accordance with the changes in methanogen composition and methanogenic pathway in this Zoige wetland sample. It appears that the aceticlastic methanogenesis dominated at low temperatures is more sensitive than the hydrogenotrophic one at higher temperatures.http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.00131/fullmethanogenesisMethanogenstibetan plateautemperature sensitivityZoige wetland |
| spellingShingle | Li eFu Tianze eSong Yahai eLu Yahai eLu Snapshot of methanogen sensitivity to temperature in Zoige wetland from Tibetan plateau Frontiers in Microbiology methanogenesis Methanogens tibetan plateau temperature sensitivity Zoige wetland |
| title | Snapshot of methanogen sensitivity to temperature in Zoige wetland from Tibetan plateau |
| title_full | Snapshot of methanogen sensitivity to temperature in Zoige wetland from Tibetan plateau |
| title_fullStr | Snapshot of methanogen sensitivity to temperature in Zoige wetland from Tibetan plateau |
| title_full_unstemmed | Snapshot of methanogen sensitivity to temperature in Zoige wetland from Tibetan plateau |
| title_short | Snapshot of methanogen sensitivity to temperature in Zoige wetland from Tibetan plateau |
| title_sort | snapshot of methanogen sensitivity to temperature in zoige wetland from tibetan plateau |
| topic | methanogenesis Methanogens tibetan plateau temperature sensitivity Zoige wetland |
| url | http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.00131/full |
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