Oral administration of quercetin and fisetin potentiates photocarcinogenesis in UVR-exposed hairless mice
Background: Phytochemicals have demonstrated great potential as photoprotectants. Apple-derived compounds such as quercetin, fisetin, and rutin are reported to provide topical photoprotection, but oral delivery has not been explored. Purpose: To determine the photoprotective effects of oral administ...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-05-01
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| Series: | Phytomedicine Plus |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667031324000253 |
| _version_ | 1797246781883416576 |
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| author | Celina Pihl Jonatan Riber Granborg Fernanda Endringer Pinto Peter Bjerring Flemming Andersen Christian Janfelt Merete Haedersdal Catharina Margrethe Lerche |
| author_facet | Celina Pihl Jonatan Riber Granborg Fernanda Endringer Pinto Peter Bjerring Flemming Andersen Christian Janfelt Merete Haedersdal Catharina Margrethe Lerche |
| author_sort | Celina Pihl |
| collection | DOAJ |
| description | Background: Phytochemicals have demonstrated great potential as photoprotectants. Apple-derived compounds such as quercetin, fisetin, and rutin are reported to provide topical photoprotection, but oral delivery has not been explored. Purpose: To determine the photoprotective effects of oral administration of quercetin, fisetin, and rutin, and their accumulation in skin assessed through mass spectrometry imaging. Study design: Groups of 25 hairless mice (n = 125 mice) received in the daily feed 100 mg/kg quercetin, fisetin, or rutin, 600 mg/kg nicotinamide in water as a positive control, or no supplementation as the UV control. The animals were exposed to ultraviolet radiation (UVR) equivalent to 3.5 standard erythema doses thrice weekly. Method: Mass spectroemetry imaging was used to assess local skin accumulation. Results: Oral administration of quercetin and fisetin reduced the time to tumour onset (Quercetin: second and third tumour [p < 0.045]; fisetin: third tumour [p < 0.021]), with no observed effect for rutin. Nicotinamide delayed the onset of all three recorded tumours (p < 0.0082). Results were supported by accelerated tumour growth following quercetin treatment (p < 0.0069), whereas nicotinamide reduced tumour growth (p < 0.00015). Skin accumulation of the compounds could not be demonstrated, suggesting other mechanisms must be explored to explain these effects on UVR-induced carcinogenesis. Conclusion: Oral administration of quercetin and fisetin to hairless mice increased UVR-induced tumour development. These results indicate a need for caution when selecting candidates for photoprotectants. |
| first_indexed | 2024-04-24T19:48:16Z |
| format | Article |
| id | doaj.art-2cbaec1ac2ed464588504c15a954f79f |
| institution | Directory Open Access Journal |
| issn | 2667-0313 |
| language | English |
| last_indexed | 2024-04-24T19:48:16Z |
| publishDate | 2024-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Phytomedicine Plus |
| spelling | doaj.art-2cbaec1ac2ed464588504c15a954f79f2024-03-25T04:18:08ZengElsevierPhytomedicine Plus2667-03132024-05-0142100547Oral administration of quercetin and fisetin potentiates photocarcinogenesis in UVR-exposed hairless miceCelina Pihl0Jonatan Riber Granborg1Fernanda Endringer Pinto2Peter Bjerring3Flemming Andersen4Christian Janfelt5Merete Haedersdal6Catharina Margrethe Lerche7Dept of Dermatology, Copenhagen University Hospital – Bispebjerg and Frederiksberg, Copenhagen, Denmark & Dept of Pharmacy, University of Copenhagen, 2400 Copenhagen, Denmark; Corresponding author at: Department of Dermatology, D92, Copenhagen University Hospital, Bispebjerg, Nielsine Nielsens Vej 17, 2400 Copenhagen, Denmark.Dept of Dermatology, Copenhagen University Hospital – Bispebjerg and Frederiksberg, Copenhagen, Denmark & Dept of Pharmacy, University of Copenhagen, 2400 Copenhagen, DenmarkDept of Dermatology, Copenhagen University Hospital – Bispebjerg and Frederiksberg, Copenhagen, Denmark & Dept of Pharmacy, University of Copenhagen, 2400 Copenhagen, DenmarkDept of Dermatology, Aalborg University Hospital, 9100 Aalborg, DenmarkDept of Dermatology, Aalborg University Hospital, 9100 Aalborg, Denmark; Dept of Dermatology, Private Hospital Molholm, 7100 Vejle, DenmarkDept of Pharmacy, University of Copenhagen, 2400 Copenhagen, DenmarkDept of Dermatology, Copenhagen University Hospital – Bispebjerg and Frederiksberg, Copenhagen, Denmark & Dept of Clinical Medicine, University of Copenhagen, 2400 Copenhagen, DenmarkDept of Dermatology, Copenhagen University Hospital – Bispebjerg and Frederiksberg, Copenhagen, Denmark & Dept of Pharmacy, University of Copenhagen, 2400 Copenhagen, DenmarkBackground: Phytochemicals have demonstrated great potential as photoprotectants. Apple-derived compounds such as quercetin, fisetin, and rutin are reported to provide topical photoprotection, but oral delivery has not been explored. Purpose: To determine the photoprotective effects of oral administration of quercetin, fisetin, and rutin, and their accumulation in skin assessed through mass spectrometry imaging. Study design: Groups of 25 hairless mice (n = 125 mice) received in the daily feed 100 mg/kg quercetin, fisetin, or rutin, 600 mg/kg nicotinamide in water as a positive control, or no supplementation as the UV control. The animals were exposed to ultraviolet radiation (UVR) equivalent to 3.5 standard erythema doses thrice weekly. Method: Mass spectroemetry imaging was used to assess local skin accumulation. Results: Oral administration of quercetin and fisetin reduced the time to tumour onset (Quercetin: second and third tumour [p < 0.045]; fisetin: third tumour [p < 0.021]), with no observed effect for rutin. Nicotinamide delayed the onset of all three recorded tumours (p < 0.0082). Results were supported by accelerated tumour growth following quercetin treatment (p < 0.0069), whereas nicotinamide reduced tumour growth (p < 0.00015). Skin accumulation of the compounds could not be demonstrated, suggesting other mechanisms must be explored to explain these effects on UVR-induced carcinogenesis. Conclusion: Oral administration of quercetin and fisetin to hairless mice increased UVR-induced tumour development. These results indicate a need for caution when selecting candidates for photoprotectants.http://www.sciencedirect.com/science/article/pii/S2667031324000253Ultraviolet radiationHairless miceSkin cancerOral deliveryPhotoprotectionPhytochemicals |
| spellingShingle | Celina Pihl Jonatan Riber Granborg Fernanda Endringer Pinto Peter Bjerring Flemming Andersen Christian Janfelt Merete Haedersdal Catharina Margrethe Lerche Oral administration of quercetin and fisetin potentiates photocarcinogenesis in UVR-exposed hairless mice Phytomedicine Plus Ultraviolet radiation Hairless mice Skin cancer Oral delivery Photoprotection Phytochemicals |
| title | Oral administration of quercetin and fisetin potentiates photocarcinogenesis in UVR-exposed hairless mice |
| title_full | Oral administration of quercetin and fisetin potentiates photocarcinogenesis in UVR-exposed hairless mice |
| title_fullStr | Oral administration of quercetin and fisetin potentiates photocarcinogenesis in UVR-exposed hairless mice |
| title_full_unstemmed | Oral administration of quercetin and fisetin potentiates photocarcinogenesis in UVR-exposed hairless mice |
| title_short | Oral administration of quercetin and fisetin potentiates photocarcinogenesis in UVR-exposed hairless mice |
| title_sort | oral administration of quercetin and fisetin potentiates photocarcinogenesis in uvr exposed hairless mice |
| topic | Ultraviolet radiation Hairless mice Skin cancer Oral delivery Photoprotection Phytochemicals |
| url | http://www.sciencedirect.com/science/article/pii/S2667031324000253 |
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