Differentiation of the sibling species Biomphalaria occidentalis and Biomphalaria tenagophila by the electrophoretic patterns of their hemoglobin
A simple and rapid method for differentialing the sibling species Biomphalaria tenagophila and Biomphalaria occidentalis by agarose gel electrophoresis (AGE) is described. Snail hemolymph is used as the test sample and the red colaration of the hemoglobin fraction permits visualization of the migrat...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Fundação Oswaldo Cruz (FIOCRUZ)
1986-09-01
|
| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761986000300008 |
| _version_ | 1797713268254441472 |
|---|---|
| author | James B. Bailey Edward H. Michelson W. Lobato Paraense |
| author_facet | James B. Bailey Edward H. Michelson W. Lobato Paraense |
| author_sort | James B. Bailey |
| collection | DOAJ |
| description | A simple and rapid method for differentialing the sibling species Biomphalaria tenagophila and Biomphalaria occidentalis by agarose gel electrophoresis (AGE) is described. Snail hemolymph is used as the test sample and the red colaration of the hemoglobin fraction permits visualization of the migration patterns without resorting to specific stains. Moreover, hemolymph samples may be obtained without killing the snail, thus permitting its use for other studies for breeding.<br>É descrito um método simples e rápido para distinguir as espécies crípticas Biomphalaria tenagophila e B. occidentalis por eletroforese em gel de agarose. A prova é feita com hemolinfa do molusco, permitindo a cor vermelha da fração hemoglobina visualizar os padrões de migração sem necessidade de recorrer a colorações específicas. Além disso, as amostras de hemolinfa podem ser obtidas sem sacrificar o molusco, que poderá ser usado para outros estudos ou para criação. |
| first_indexed | 2024-03-12T07:33:55Z |
| format | Article |
| id | doaj.art-2cbec9355f2a42c6901e7ae4cc0f66e2 |
| institution | Directory Open Access Journal |
| issn | 0074-0276 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T07:33:55Z |
| publishDate | 1986-09-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-2cbec9355f2a42c6901e7ae4cc0f66e22023-09-02T21:33:31ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz0074-02761678-80601986-09-0181331932210.1590/S0074-02761986000300008Differentiation of the sibling species Biomphalaria occidentalis and Biomphalaria tenagophila by the electrophoretic patterns of their hemoglobinJames B. BaileyEdward H. MichelsonW. Lobato ParaenseA simple and rapid method for differentialing the sibling species Biomphalaria tenagophila and Biomphalaria occidentalis by agarose gel electrophoresis (AGE) is described. Snail hemolymph is used as the test sample and the red colaration of the hemoglobin fraction permits visualization of the migration patterns without resorting to specific stains. Moreover, hemolymph samples may be obtained without killing the snail, thus permitting its use for other studies for breeding.<br>É descrito um método simples e rápido para distinguir as espécies crípticas Biomphalaria tenagophila e B. occidentalis por eletroforese em gel de agarose. A prova é feita com hemolinfa do molusco, permitindo a cor vermelha da fração hemoglobina visualizar os padrões de migração sem necessidade de recorrer a colorações específicas. Além disso, as amostras de hemolinfa podem ser obtidas sem sacrificar o molusco, que poderá ser usado para outros estudos ou para criação.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761986000300008Biomphalaria occidentalisBiomphalaria tenagophilasibling specieshemoglobinelectrophoresisbiochemical taxonomy |
| spellingShingle | James B. Bailey Edward H. Michelson W. Lobato Paraense Differentiation of the sibling species Biomphalaria occidentalis and Biomphalaria tenagophila by the electrophoretic patterns of their hemoglobin Memorias do Instituto Oswaldo Cruz Biomphalaria occidentalis Biomphalaria tenagophila sibling species hemoglobin electrophoresis biochemical taxonomy |
| title | Differentiation of the sibling species Biomphalaria occidentalis and Biomphalaria tenagophila by the electrophoretic patterns of their hemoglobin |
| title_full | Differentiation of the sibling species Biomphalaria occidentalis and Biomphalaria tenagophila by the electrophoretic patterns of their hemoglobin |
| title_fullStr | Differentiation of the sibling species Biomphalaria occidentalis and Biomphalaria tenagophila by the electrophoretic patterns of their hemoglobin |
| title_full_unstemmed | Differentiation of the sibling species Biomphalaria occidentalis and Biomphalaria tenagophila by the electrophoretic patterns of their hemoglobin |
| title_short | Differentiation of the sibling species Biomphalaria occidentalis and Biomphalaria tenagophila by the electrophoretic patterns of their hemoglobin |
| title_sort | differentiation of the sibling species biomphalaria occidentalis and biomphalaria tenagophila by the electrophoretic patterns of their hemoglobin |
| topic | Biomphalaria occidentalis Biomphalaria tenagophila sibling species hemoglobin electrophoresis biochemical taxonomy |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761986000300008 |
| work_keys_str_mv | AT jamesbbailey differentiationofthesiblingspeciesbiomphalariaoccidentalisandbiomphalariatenagophilabytheelectrophoreticpatternsoftheirhemoglobin AT edwardhmichelson differentiationofthesiblingspeciesbiomphalariaoccidentalisandbiomphalariatenagophilabytheelectrophoreticpatternsoftheirhemoglobin AT wlobatoparaense differentiationofthesiblingspeciesbiomphalariaoccidentalisandbiomphalariatenagophilabytheelectrophoreticpatternsoftheirhemoglobin |