Ribosome profiling enhances understanding of mycobacterial translation
A recent addition to the -omics toolkit, ribosome profiling, enables researchers to gain insight into the process and regulation of translation by mapping fragments of mRNA protected from nuclease digestion by ribosome binding. In this review, we discuss how ribosome profiling applied to mycobacteri...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-08-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.976550/full |
| _version_ | 1811221319160692736 |
|---|---|
| author | Elizabeth B. Sawyer Teresa Cortes |
| author_facet | Elizabeth B. Sawyer Teresa Cortes |
| author_sort | Elizabeth B. Sawyer |
| collection | DOAJ |
| description | A recent addition to the -omics toolkit, ribosome profiling, enables researchers to gain insight into the process and regulation of translation by mapping fragments of mRNA protected from nuclease digestion by ribosome binding. In this review, we discuss how ribosome profiling applied to mycobacteria has led to discoveries about translational regulation. Using case studies, we show that the traditional view of “canonical” translation mechanisms needs expanding to encompass features of mycobacterial translation that are more widespread than previously recognized. We also discuss the limitations of the method and potential future developments that could yield further insight into the fundamental biology of this important human pathogen. |
| first_indexed | 2024-04-12T07:57:23Z |
| format | Article |
| id | doaj.art-2cbfebb3b6734c47b18f5696898f3b6d |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-12T07:57:23Z |
| publishDate | 2022-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-2cbfebb3b6734c47b18f5696898f3b6d2022-12-22T03:41:26ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-08-011310.3389/fmicb.2022.976550976550Ribosome profiling enhances understanding of mycobacterial translationElizabeth B. Sawyer0Teresa Cortes1School of Life Sciences, University of Westminster, London, United KingdomPathogen Gene Regulation Unit, Instituto de Biomedicina de Valencia (IBV), CSIC, Valencia, SpainA recent addition to the -omics toolkit, ribosome profiling, enables researchers to gain insight into the process and regulation of translation by mapping fragments of mRNA protected from nuclease digestion by ribosome binding. In this review, we discuss how ribosome profiling applied to mycobacteria has led to discoveries about translational regulation. Using case studies, we show that the traditional view of “canonical” translation mechanisms needs expanding to encompass features of mycobacterial translation that are more widespread than previously recognized. We also discuss the limitations of the method and potential future developments that could yield further insight into the fundamental biology of this important human pathogen.https://www.frontiersin.org/articles/10.3389/fmicb.2022.976550/fullribosome profilingMycobacterium tuberculosisnon-canonical translationtranslation initiationomics |
| spellingShingle | Elizabeth B. Sawyer Teresa Cortes Ribosome profiling enhances understanding of mycobacterial translation Frontiers in Microbiology ribosome profiling Mycobacterium tuberculosis non-canonical translation translation initiation omics |
| title | Ribosome profiling enhances understanding of mycobacterial translation |
| title_full | Ribosome profiling enhances understanding of mycobacterial translation |
| title_fullStr | Ribosome profiling enhances understanding of mycobacterial translation |
| title_full_unstemmed | Ribosome profiling enhances understanding of mycobacterial translation |
| title_short | Ribosome profiling enhances understanding of mycobacterial translation |
| title_sort | ribosome profiling enhances understanding of mycobacterial translation |
| topic | ribosome profiling Mycobacterium tuberculosis non-canonical translation translation initiation omics |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.976550/full |
| work_keys_str_mv | AT elizabethbsawyer ribosomeprofilingenhancesunderstandingofmycobacterialtranslation AT teresacortes ribosomeprofilingenhancesunderstandingofmycobacterialtranslation |