Stem Cutting Propagation and Micropropagation of Northern Bayberry
Northern bayberry [Morella (formerly Myrica) pensylvanica] is an attractive, adaptable, semievergreen, northeastern North American native shrub that is sought for landscaping but difficult to propagate clonally. The impact of timing (June, July, or August) and concentration of indole-3-butyric acid...
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| Format: | Article |
| Language: | English |
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American Society for Horticultural Science (ASHS)
2024-02-01
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| Series: | HortScience |
| Subjects: | |
| Online Access: | https://journals.ashs.org/hortsci/view/journals/hortsci/59/4/article-p483.xml |
| _version_ | 1797260732165783552 |
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| author | Jackie D. Edgett Jessica D. Lubell-Brand Mark H. Brand |
| author_facet | Jackie D. Edgett Jessica D. Lubell-Brand Mark H. Brand |
| author_sort | Jackie D. Edgett |
| collection | DOAJ |
| description | Northern bayberry [Morella (formerly Myrica) pensylvanica] is an attractive, adaptable, semievergreen, northeastern North American native shrub that is sought for landscaping but difficult to propagate clonally. The impact of timing (June, July, or August) and concentration of indole-3-butyric acid [IBA (0, 2000, 4000 or 8000 ppm)] on propagation by stem cuttings was evaluated for genotypes of northern bayberry including the female cultivars Bobzam (Bobbee™) and UConn Compact and an unnamed male. Medium formulation and cytokinin type were evaluated for micropropagation of ‘Bobzam’ and ‘UConn Compact’. Stem cuttings of ‘Bobzam’ and ‘UConn Compact’ rooted poorly (at ≤55% and ≤20%, respectively) at all timings and concentrations of IBA; however, rooting success of ≥85% was achieved for the unnamed male genotype when cuttings were taken in June. Micropropagation of ‘Bobzam’ was successful using Woody Plant medium with 4 mg·L−1 zeatin and explants taken from shoots that had expanded 12 to 18 cm on containerized stock plants. Initiated explants of ‘Bobzam’ required eight subcultures before they began to produce shoots consistently at a 2× multiplication rate and eventually reached a 3× multiplication rate. Micropropagation attempts using Murashige and Skoog medium, the cytokinins 6-benzylaminopurine, meta-topolin, and thidiazuron, or the cultivar ‘UConn Compact’ were unsuccessful. Microshoots of ‘Bobzam’ rooted at ≥80% either by in vitro prerooting or ex vitro rooting directly in trays. Rooted microcuttings easily acclimated to greenhouse conditions and grew rapidly when potted to 1.04-L containers and then into 5.68-L containers. The micropropagation protocol developed for ‘Bobzam’ can be used by propagators to expand production of this popular female cultivar. |
| first_indexed | 2024-04-24T23:30:00Z |
| format | Article |
| id | doaj.art-2cc266c8b9b14e9394f7e73391b9be79 |
| institution | Directory Open Access Journal |
| issn | 2327-9834 |
| language | English |
| last_indexed | 2024-04-24T23:30:00Z |
| publishDate | 2024-02-01 |
| publisher | American Society for Horticultural Science (ASHS) |
| record_format | Article |
| series | HortScience |
| spelling | doaj.art-2cc266c8b9b14e9394f7e73391b9be792024-03-15T15:46:56ZengAmerican Society for Horticultural Science (ASHS)HortScience2327-98342024-02-01594https://doi.org/10.21273/HORTSCI17625-23Stem Cutting Propagation and Micropropagation of Northern BayberryJackie D. Edgett0Jessica D. Lubell-Brand1Mark H. Brand2Department of Plant Science and Landscape Architecture, University of ConnecticutDepartment of Plant Science and Landscape Architecture, University of ConnecticutDepartment of Plant Science and Landscape Architecture, University of ConnecticutNorthern bayberry [Morella (formerly Myrica) pensylvanica] is an attractive, adaptable, semievergreen, northeastern North American native shrub that is sought for landscaping but difficult to propagate clonally. The impact of timing (June, July, or August) and concentration of indole-3-butyric acid [IBA (0, 2000, 4000 or 8000 ppm)] on propagation by stem cuttings was evaluated for genotypes of northern bayberry including the female cultivars Bobzam (Bobbee™) and UConn Compact and an unnamed male. Medium formulation and cytokinin type were evaluated for micropropagation of ‘Bobzam’ and ‘UConn Compact’. Stem cuttings of ‘Bobzam’ and ‘UConn Compact’ rooted poorly (at ≤55% and ≤20%, respectively) at all timings and concentrations of IBA; however, rooting success of ≥85% was achieved for the unnamed male genotype when cuttings were taken in June. Micropropagation of ‘Bobzam’ was successful using Woody Plant medium with 4 mg·L−1 zeatin and explants taken from shoots that had expanded 12 to 18 cm on containerized stock plants. Initiated explants of ‘Bobzam’ required eight subcultures before they began to produce shoots consistently at a 2× multiplication rate and eventually reached a 3× multiplication rate. Micropropagation attempts using Murashige and Skoog medium, the cytokinins 6-benzylaminopurine, meta-topolin, and thidiazuron, or the cultivar ‘UConn Compact’ were unsuccessful. Microshoots of ‘Bobzam’ rooted at ≥80% either by in vitro prerooting or ex vitro rooting directly in trays. Rooted microcuttings easily acclimated to greenhouse conditions and grew rapidly when potted to 1.04-L containers and then into 5.68-L containers. The micropropagation protocol developed for ‘Bobzam’ can be used by propagators to expand production of this popular female cultivar.https://journals.ashs.org/hortsci/view/journals/hortsci/59/4/article-p483.xmlmorella pensylvanicanative plantstissue culture |
| spellingShingle | Jackie D. Edgett Jessica D. Lubell-Brand Mark H. Brand Stem Cutting Propagation and Micropropagation of Northern Bayberry HortScience morella pensylvanica native plants tissue culture |
| title | Stem Cutting Propagation and Micropropagation of Northern Bayberry |
| title_full | Stem Cutting Propagation and Micropropagation of Northern Bayberry |
| title_fullStr | Stem Cutting Propagation and Micropropagation of Northern Bayberry |
| title_full_unstemmed | Stem Cutting Propagation and Micropropagation of Northern Bayberry |
| title_short | Stem Cutting Propagation and Micropropagation of Northern Bayberry |
| title_sort | stem cutting propagation and micropropagation of northern bayberry |
| topic | morella pensylvanica native plants tissue culture |
| url | https://journals.ashs.org/hortsci/view/journals/hortsci/59/4/article-p483.xml |
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