Optimization of growth and bacteriocin activity of the food bioprotective Carnobacterium divergens V41 in an animal origin protein free medium
Optimization of Carnobacterium divergens V41 growth and bacteriocin activity in a culture medium deprived of animal protein, needs for food bioprotection, was performed by using a statistical approach. In a screening experiment, twelve factors (pH, temperature, carbohydrates, NaCl, yeast extract, so...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2016-08-01
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| Series: | Frontiers in Marine Science |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fmars.2016.00128/full |
| _version_ | 1818128155964080128 |
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| author | Anne BRILLET-VIEL Marie-France Pilet Philippe Courcoux Hervé Prévost Françoise Leroi |
| author_facet | Anne BRILLET-VIEL Marie-France Pilet Philippe Courcoux Hervé Prévost Françoise Leroi |
| author_sort | Anne BRILLET-VIEL |
| collection | DOAJ |
| description | Optimization of Carnobacterium divergens V41 growth and bacteriocin activity in a culture medium deprived of animal protein, needs for food bioprotection, was performed by using a statistical approach. In a screening experiment, twelve factors (pH, temperature, carbohydrates, NaCl, yeast extract, soy peptone, sodium acetate, ammonium citrate, magnesium sulphate, manganese sulphate, ascorbic acid and thiamine) were tested for their influence on the maximal growth and bacteriocin activity using a two-level incomplete factorial design with 192 experiments performed in microtiter plate wells. Based on results, a basic medium was developed and three variables (pH, temperature and carbohydrates concentration) were selected for a scale-up study in bioreactor. A 23 complete factorial design was performed, allowing the estimation of linear effects of factors and all the first order interactions. The best conditions for the cell production were obtained with a temperature of 15°C and a carbohydrates concentration of 20 g/l whatever the pH (in the range 6.5-8), and the best conditions for bacteriocin activity were obtained at 15°C and pH 6.5 whatever the carbohydrates concentration (in the range 2-20 g/l). The predicted final count of C. divergens V41 and the bacteriocin activity under the optimized conditions (15°C, pH 6.5, 20 g/l carbohydrates) were 2.4 x 1010 CFU/ml and 819200 AU/ml respectively. C. divergens V41 cells cultivated in the optimized conditions were able to grow in cold-smoked salmon and totally inhibited the growth of Listeria monocytogenes (< 50 CFU g-1) during five weeks of vacuum storage at 4° and 8°C. |
| first_indexed | 2024-12-11T07:28:46Z |
| format | Article |
| id | doaj.art-2cc4bb5ad57346d18ea99ca053cad9fc |
| institution | Directory Open Access Journal |
| issn | 2296-7745 |
| language | English |
| last_indexed | 2024-12-11T07:28:46Z |
| publishDate | 2016-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Marine Science |
| spelling | doaj.art-2cc4bb5ad57346d18ea99ca053cad9fc2022-12-22T01:15:53ZengFrontiers Media S.A.Frontiers in Marine Science2296-77452016-08-01310.3389/fmars.2016.00128198584Optimization of growth and bacteriocin activity of the food bioprotective Carnobacterium divergens V41 in an animal origin protein free mediumAnne BRILLET-VIEL0Marie-France Pilet1Philippe Courcoux2Hervé Prévost3Françoise Leroi4University Bretagne LoireUniversity Bretagne LoireUniversity Bretagne LoireUniversity Bretagne LoireIfremerOptimization of Carnobacterium divergens V41 growth and bacteriocin activity in a culture medium deprived of animal protein, needs for food bioprotection, was performed by using a statistical approach. In a screening experiment, twelve factors (pH, temperature, carbohydrates, NaCl, yeast extract, soy peptone, sodium acetate, ammonium citrate, magnesium sulphate, manganese sulphate, ascorbic acid and thiamine) were tested for their influence on the maximal growth and bacteriocin activity using a two-level incomplete factorial design with 192 experiments performed in microtiter plate wells. Based on results, a basic medium was developed and three variables (pH, temperature and carbohydrates concentration) were selected for a scale-up study in bioreactor. A 23 complete factorial design was performed, allowing the estimation of linear effects of factors and all the first order interactions. The best conditions for the cell production were obtained with a temperature of 15°C and a carbohydrates concentration of 20 g/l whatever the pH (in the range 6.5-8), and the best conditions for bacteriocin activity were obtained at 15°C and pH 6.5 whatever the carbohydrates concentration (in the range 2-20 g/l). The predicted final count of C. divergens V41 and the bacteriocin activity under the optimized conditions (15°C, pH 6.5, 20 g/l carbohydrates) were 2.4 x 1010 CFU/ml and 819200 AU/ml respectively. C. divergens V41 cells cultivated in the optimized conditions were able to grow in cold-smoked salmon and totally inhibited the growth of Listeria monocytogenes (< 50 CFU g-1) during five weeks of vacuum storage at 4° and 8°C.http://journal.frontiersin.org/Journal/10.3389/fmars.2016.00128/fullCarnobacteriumGrowthoptimizationbacteriocinbioreactorexperimental design |
| spellingShingle | Anne BRILLET-VIEL Marie-France Pilet Philippe Courcoux Hervé Prévost Françoise Leroi Optimization of growth and bacteriocin activity of the food bioprotective Carnobacterium divergens V41 in an animal origin protein free medium Frontiers in Marine Science Carnobacterium Growth optimization bacteriocin bioreactor experimental design |
| title | Optimization of growth and bacteriocin activity of the food bioprotective Carnobacterium divergens V41 in an animal origin protein free medium |
| title_full | Optimization of growth and bacteriocin activity of the food bioprotective Carnobacterium divergens V41 in an animal origin protein free medium |
| title_fullStr | Optimization of growth and bacteriocin activity of the food bioprotective Carnobacterium divergens V41 in an animal origin protein free medium |
| title_full_unstemmed | Optimization of growth and bacteriocin activity of the food bioprotective Carnobacterium divergens V41 in an animal origin protein free medium |
| title_short | Optimization of growth and bacteriocin activity of the food bioprotective Carnobacterium divergens V41 in an animal origin protein free medium |
| title_sort | optimization of growth and bacteriocin activity of the food bioprotective carnobacterium divergens v41 in an animal origin protein free medium |
| topic | Carnobacterium Growth optimization bacteriocin bioreactor experimental design |
| url | http://journal.frontiersin.org/Journal/10.3389/fmars.2016.00128/full |
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