Challenges and Costs of Asexuality: Variation in Premeiotic Genome Duplication in Gynogenetic Hybrids from <i>Cobitis taenia</i> Complex
The transition from sexual reproduction to asexuality is often triggered by hybridization. The gametogenesis of many hybrid asexuals involves premeiotic genome endoreplication leading to bypass hybrid sterility and forming clonal gametes. However, it is still not clear when endoreplication occurs, h...
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MDPI AG
2021-11-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/22/12117 |
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| author | Dmitrij Dedukh Anatolie Marta Karel Janko |
| author_facet | Dmitrij Dedukh Anatolie Marta Karel Janko |
| author_sort | Dmitrij Dedukh |
| collection | DOAJ |
| description | The transition from sexual reproduction to asexuality is often triggered by hybridization. The gametogenesis of many hybrid asexuals involves premeiotic genome endoreplication leading to bypass hybrid sterility and forming clonal gametes. However, it is still not clear when endoreplication occurs, how many gonial cells it affects and whether its rate differs among clonal lineages. Here, we investigated meiotic and premeiotic cells of diploid and triploid hybrids of spined loaches (Cypriniformes: <i>Cobitis</i>) that reproduce by gynogenesis. We found that in naturally and experimentally produced F1 hybrids asexuality is achieved by genome endoreplication, which occurs in gonocytes just before entering meiosis or, rarely, one or a few divisions before meiosis. However, genome endoreplication was observed only in a minor fraction of the hybrid’s gonocytes, while the vast majority of gonocytes were unable to duplicate their genomes and consequently could not proceed beyond pachytene due to defects in bivalent formation. We also noted that the rate of endoreplication was significantly higher among gonocytes of hybrids from natural clones than of experimentally produced F1 hybrids. Thus, asexuality and hybrid sterility are intimately related phenomena and the transition from sexual reproduction to asexuality must overcome significant problems with genome incompatibilities with a possible impact on reproductive potential. |
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| id | doaj.art-2cc5211edafd4bb08e3278c2c5ddfe03 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T05:26:22Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-2cc5211edafd4bb08e3278c2c5ddfe032023-11-22T23:37:32ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-11-0122221211710.3390/ijms222212117Challenges and Costs of Asexuality: Variation in Premeiotic Genome Duplication in Gynogenetic Hybrids from <i>Cobitis taenia</i> ComplexDmitrij Dedukh0Anatolie Marta1Karel Janko2Laboratory of Fish Genetics, Institute of Animal Physiology and Genetics of the CAS, Rumburská 89, 277 21 Liběchov, Czech RepublicLaboratory of Fish Genetics, Institute of Animal Physiology and Genetics of the CAS, Rumburská 89, 277 21 Liběchov, Czech RepublicLaboratory of Fish Genetics, Institute of Animal Physiology and Genetics of the CAS, Rumburská 89, 277 21 Liběchov, Czech RepublicThe transition from sexual reproduction to asexuality is often triggered by hybridization. The gametogenesis of many hybrid asexuals involves premeiotic genome endoreplication leading to bypass hybrid sterility and forming clonal gametes. However, it is still not clear when endoreplication occurs, how many gonial cells it affects and whether its rate differs among clonal lineages. Here, we investigated meiotic and premeiotic cells of diploid and triploid hybrids of spined loaches (Cypriniformes: <i>Cobitis</i>) that reproduce by gynogenesis. We found that in naturally and experimentally produced F1 hybrids asexuality is achieved by genome endoreplication, which occurs in gonocytes just before entering meiosis or, rarely, one or a few divisions before meiosis. However, genome endoreplication was observed only in a minor fraction of the hybrid’s gonocytes, while the vast majority of gonocytes were unable to duplicate their genomes and consequently could not proceed beyond pachytene due to defects in bivalent formation. We also noted that the rate of endoreplication was significantly higher among gonocytes of hybrids from natural clones than of experimentally produced F1 hybrids. Thus, asexuality and hybrid sterility are intimately related phenomena and the transition from sexual reproduction to asexuality must overcome significant problems with genome incompatibilities with a possible impact on reproductive potential.https://www.mdpi.com/1422-0067/22/22/12117gynogenesismeiosishybrid sterilityendoreplicationpolyploidy<i>Cobitis taenia</i> complex |
| spellingShingle | Dmitrij Dedukh Anatolie Marta Karel Janko Challenges and Costs of Asexuality: Variation in Premeiotic Genome Duplication in Gynogenetic Hybrids from <i>Cobitis taenia</i> Complex International Journal of Molecular Sciences gynogenesis meiosis hybrid sterility endoreplication polyploidy <i>Cobitis taenia</i> complex |
| title | Challenges and Costs of Asexuality: Variation in Premeiotic Genome Duplication in Gynogenetic Hybrids from <i>Cobitis taenia</i> Complex |
| title_full | Challenges and Costs of Asexuality: Variation in Premeiotic Genome Duplication in Gynogenetic Hybrids from <i>Cobitis taenia</i> Complex |
| title_fullStr | Challenges and Costs of Asexuality: Variation in Premeiotic Genome Duplication in Gynogenetic Hybrids from <i>Cobitis taenia</i> Complex |
| title_full_unstemmed | Challenges and Costs of Asexuality: Variation in Premeiotic Genome Duplication in Gynogenetic Hybrids from <i>Cobitis taenia</i> Complex |
| title_short | Challenges and Costs of Asexuality: Variation in Premeiotic Genome Duplication in Gynogenetic Hybrids from <i>Cobitis taenia</i> Complex |
| title_sort | challenges and costs of asexuality variation in premeiotic genome duplication in gynogenetic hybrids from i cobitis taenia i complex |
| topic | gynogenesis meiosis hybrid sterility endoreplication polyploidy <i>Cobitis taenia</i> complex |
| url | https://www.mdpi.com/1422-0067/22/22/12117 |
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