Temperature-Inducible Transgenic <i>EDS1</i> and <i>PAD4</i> in <i>Arabidopsi</i><i>s</i> Confer an Enhanced Disease Resistance at Elevated Temperature
Temperature is one of the most important environmental factors greatly affecting plant disease development. High temperature favors outbreaks of many plant diseases, which threaten food security and turn to be a big issue along with climate change and global warming. Here, we found that concurrent c...
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MDPI AG
2021-06-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/10/6/1258 |
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| author | Junchen Leng Weishan Tu Yanbing Hou Haitao Cui |
| author_facet | Junchen Leng Weishan Tu Yanbing Hou Haitao Cui |
| author_sort | Junchen Leng |
| collection | DOAJ |
| description | Temperature is one of the most important environmental factors greatly affecting plant disease development. High temperature favors outbreaks of many plant diseases, which threaten food security and turn to be a big issue along with climate change and global warming. Here, we found that concurrent constitutive expression of the key immune regulators <i>EDS1</i> and <i>PAD4</i> in <i>Arabidopsis</i> significantly enhanced resistance to virulent bacterial pathogen <i>Pseudomonas syringae</i> pv. <i>tomato</i> at elevated temperature; however, autoimmunity-related growth retardation was also observed on these plants at a normal temperature. To balance this growth-defense trade-off, we generated transgenic plants dual expressing <i>EDS1</i> and <i>PAD4</i> genes under the control of a thermo-sensitive promoter from the <i>HSP70</i> gene, whose expression is highly induced at an elevated temperature. Unlike constitutive overexpression lines, the proHSP70-EP transgenic lines exhibited enhanced resistance to bacterial pathogens at an elevated temperature without growth defects at normal condition. Thus, this study provides a potential strategy for genetic manipulation of plants to deal with the simultaneous abiotic and biotic stresses. |
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| language | English |
| last_indexed | 2024-03-10T10:12:27Z |
| publishDate | 2021-06-01 |
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| spelling | doaj.art-2cc59d1d4b5f4440aee95a62e978156f2023-11-22T01:02:37ZengMDPI AGPlants2223-77472021-06-01106125810.3390/plants10061258Temperature-Inducible Transgenic <i>EDS1</i> and <i>PAD4</i> in <i>Arabidopsi</i><i>s</i> Confer an Enhanced Disease Resistance at Elevated TemperatureJunchen Leng0Weishan Tu1Yanbing Hou2Haitao Cui3Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, Plant Immunity Center, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaKey Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, Plant Immunity Center, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaKey Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, Plant Immunity Center, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaKey Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, Plant Immunity Center, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaTemperature is one of the most important environmental factors greatly affecting plant disease development. High temperature favors outbreaks of many plant diseases, which threaten food security and turn to be a big issue along with climate change and global warming. Here, we found that concurrent constitutive expression of the key immune regulators <i>EDS1</i> and <i>PAD4</i> in <i>Arabidopsis</i> significantly enhanced resistance to virulent bacterial pathogen <i>Pseudomonas syringae</i> pv. <i>tomato</i> at elevated temperature; however, autoimmunity-related growth retardation was also observed on these plants at a normal temperature. To balance this growth-defense trade-off, we generated transgenic plants dual expressing <i>EDS1</i> and <i>PAD4</i> genes under the control of a thermo-sensitive promoter from the <i>HSP70</i> gene, whose expression is highly induced at an elevated temperature. Unlike constitutive overexpression lines, the proHSP70-EP transgenic lines exhibited enhanced resistance to bacterial pathogens at an elevated temperature without growth defects at normal condition. Thus, this study provides a potential strategy for genetic manipulation of plants to deal with the simultaneous abiotic and biotic stresses.https://www.mdpi.com/2223-7747/10/6/1258temperaturedisease resistanceimmunityEDS1PAD4 |
| spellingShingle | Junchen Leng Weishan Tu Yanbing Hou Haitao Cui Temperature-Inducible Transgenic <i>EDS1</i> and <i>PAD4</i> in <i>Arabidopsi</i><i>s</i> Confer an Enhanced Disease Resistance at Elevated Temperature Plants temperature disease resistance immunity EDS1 PAD4 |
| title | Temperature-Inducible Transgenic <i>EDS1</i> and <i>PAD4</i> in <i>Arabidopsi</i><i>s</i> Confer an Enhanced Disease Resistance at Elevated Temperature |
| title_full | Temperature-Inducible Transgenic <i>EDS1</i> and <i>PAD4</i> in <i>Arabidopsi</i><i>s</i> Confer an Enhanced Disease Resistance at Elevated Temperature |
| title_fullStr | Temperature-Inducible Transgenic <i>EDS1</i> and <i>PAD4</i> in <i>Arabidopsi</i><i>s</i> Confer an Enhanced Disease Resistance at Elevated Temperature |
| title_full_unstemmed | Temperature-Inducible Transgenic <i>EDS1</i> and <i>PAD4</i> in <i>Arabidopsi</i><i>s</i> Confer an Enhanced Disease Resistance at Elevated Temperature |
| title_short | Temperature-Inducible Transgenic <i>EDS1</i> and <i>PAD4</i> in <i>Arabidopsi</i><i>s</i> Confer an Enhanced Disease Resistance at Elevated Temperature |
| title_sort | temperature inducible transgenic i eds1 i and i pad4 i in i arabidopsi i i s i confer an enhanced disease resistance at elevated temperature |
| topic | temperature disease resistance immunity EDS1 PAD4 |
| url | https://www.mdpi.com/2223-7747/10/6/1258 |
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