Automated Hematological Approach and Protein Electrophoretic Pattern in Tilapia (<i>Oreochromis niloticus</i>): An Innovative and Experimental Model for Aquaculture
This study aimed to assess the usefulness of two innovative automated methods (automated blood count counters and flow cytometry) for hematological investigation in Tilapia to make a contribution to the clinical diagnostics of this farmed species. Moreover, serum total proteins and their electrophor...
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MDPI AG
2024-01-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/14/3/392 |
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| author | Francesco Fazio Antonino Costa Fabiano Capparucci Gregorio Costa Vincenzo Parrino Francesca Arfuso |
| author_facet | Francesco Fazio Antonino Costa Fabiano Capparucci Gregorio Costa Vincenzo Parrino Francesca Arfuso |
| author_sort | Francesco Fazio |
| collection | DOAJ |
| description | This study aimed to assess the usefulness of two innovative automated methods (automated blood count counters and flow cytometry) for hematological investigation in Tilapia to make a contribution to the clinical diagnostics of this farmed species. Moreover, serum total proteins and their electrophoretic fractions (prealbumin, albumin, α-, β-, and γ-fraction), as health condition indicators, were assessed. The analysis of serum total proteins and electrophoretic fraction showed a normal and typical electrophoretic pattern of healthy fish (serum total proteins, 3.70 ± 0.62 g/dL; prealbumin, 0.44 ± 0.20 g/dL; albumin, 1.17 ± 0.66 g/dL; α-fraction, 1.49 ± 0.64 g/dL; β-fraction, 0.32 ± 0.16 g/dL; and γ-fraction, 0.29 ± 0.13 g/dL). The relationships between the values of red blood cells (RBCs), white blood cells (WBCs), and thrombocytes (TCs) obtained with the two automated methods were determined using Pearson correlation analysis. The results showed a significant positive correlation between automatic blood cell counting and flow cytometry analysis for RBCs (r = 0.97, <i>p</i> < 0.0001) and WBCs (r = 0.91, <i>p</i> < 0.0001), whereas no correlation was found for TCs (r = −0.11, <i>p</i> = 0.66). The preliminary results gathered in this study seem to highlight the usefulness of the new analytical techniques herein investigated in tilapia, suggesting their application in the hematological investigation of farmed fish species and their usefulness for monitoring the health and well-being of fish reared in aquaculture. |
| first_indexed | 2024-03-08T04:01:18Z |
| format | Article |
| id | doaj.art-2cc61a671b834e2eacb42b2ffb340899 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-08T04:01:18Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-2cc61a671b834e2eacb42b2ffb3408992024-02-09T15:06:27ZengMDPI AGAnimals2076-26152024-01-0114339210.3390/ani14030392Automated Hematological Approach and Protein Electrophoretic Pattern in Tilapia (<i>Oreochromis niloticus</i>): An Innovative and Experimental Model for AquacultureFrancesco Fazio0Antonino Costa1Fabiano Capparucci2Gregorio Costa3Vincenzo Parrino4Francesca Arfuso5Department of Veterinary Sciences, University of Messina, Via Palatucci, 98168 Messina, ItalyVeterinary Practitioner, 98100 Messina, ItalyDepartment of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, 98166 Messina, ItalyDepartment of Human Pathology in Adult and Developmental Age, University of Messina, 98125 Messina, ItalyDepartment of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, 98166 Messina, ItalyDepartment of Veterinary Sciences, University of Messina, Via Palatucci, 98168 Messina, ItalyThis study aimed to assess the usefulness of two innovative automated methods (automated blood count counters and flow cytometry) for hematological investigation in Tilapia to make a contribution to the clinical diagnostics of this farmed species. Moreover, serum total proteins and their electrophoretic fractions (prealbumin, albumin, α-, β-, and γ-fraction), as health condition indicators, were assessed. The analysis of serum total proteins and electrophoretic fraction showed a normal and typical electrophoretic pattern of healthy fish (serum total proteins, 3.70 ± 0.62 g/dL; prealbumin, 0.44 ± 0.20 g/dL; albumin, 1.17 ± 0.66 g/dL; α-fraction, 1.49 ± 0.64 g/dL; β-fraction, 0.32 ± 0.16 g/dL; and γ-fraction, 0.29 ± 0.13 g/dL). The relationships between the values of red blood cells (RBCs), white blood cells (WBCs), and thrombocytes (TCs) obtained with the two automated methods were determined using Pearson correlation analysis. The results showed a significant positive correlation between automatic blood cell counting and flow cytometry analysis for RBCs (r = 0.97, <i>p</i> < 0.0001) and WBCs (r = 0.91, <i>p</i> < 0.0001), whereas no correlation was found for TCs (r = −0.11, <i>p</i> = 0.66). The preliminary results gathered in this study seem to highlight the usefulness of the new analytical techniques herein investigated in tilapia, suggesting their application in the hematological investigation of farmed fish species and their usefulness for monitoring the health and well-being of fish reared in aquaculture.https://www.mdpi.com/2076-2615/14/3/392hematologyelectrophoretic proteinsblood cellsfarmed fish |
| spellingShingle | Francesco Fazio Antonino Costa Fabiano Capparucci Gregorio Costa Vincenzo Parrino Francesca Arfuso Automated Hematological Approach and Protein Electrophoretic Pattern in Tilapia (<i>Oreochromis niloticus</i>): An Innovative and Experimental Model for Aquaculture Animals hematology electrophoretic proteins blood cells farmed fish |
| title | Automated Hematological Approach and Protein Electrophoretic Pattern in Tilapia (<i>Oreochromis niloticus</i>): An Innovative and Experimental Model for Aquaculture |
| title_full | Automated Hematological Approach and Protein Electrophoretic Pattern in Tilapia (<i>Oreochromis niloticus</i>): An Innovative and Experimental Model for Aquaculture |
| title_fullStr | Automated Hematological Approach and Protein Electrophoretic Pattern in Tilapia (<i>Oreochromis niloticus</i>): An Innovative and Experimental Model for Aquaculture |
| title_full_unstemmed | Automated Hematological Approach and Protein Electrophoretic Pattern in Tilapia (<i>Oreochromis niloticus</i>): An Innovative and Experimental Model for Aquaculture |
| title_short | Automated Hematological Approach and Protein Electrophoretic Pattern in Tilapia (<i>Oreochromis niloticus</i>): An Innovative and Experimental Model for Aquaculture |
| title_sort | automated hematological approach and protein electrophoretic pattern in tilapia i oreochromis niloticus i an innovative and experimental model for aquaculture |
| topic | hematology electrophoretic proteins blood cells farmed fish |
| url | https://www.mdpi.com/2076-2615/14/3/392 |
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