A Novel Ochratoxin A Aptasensor Based on Three-Dimensionally Ordered Macroporous RGO-AuNPs-MoS<sub>2</sub> Enhanced Electrocatalysis of Methylene Blue and AuNPs-Fe<sub>3</sub>O<sub>4</sub>@C Composite as Signal Probe Carrier
In this work, a novel electrochemical aptasensor for the detection of ochratoxin A (OTA) was developed using a three-dimensionally ordered macroporous (3DOM) reduced graphene oxide–Au nanoparticles–molybdenum disulfide (RGO-AuNPs-MoS<sub>2</sub>) composite material as the sensing interfa...
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MDPI AG
2023-07-01
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| Series: | Catalysts |
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| Online Access: | https://www.mdpi.com/2073-4344/13/7/1088 |
| _version_ | 1797589879623778304 |
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| author | Gang Peng Xu Zhang Kaiwen Zhang Xiaojun Chen He Huang |
| author_facet | Gang Peng Xu Zhang Kaiwen Zhang Xiaojun Chen He Huang |
| author_sort | Gang Peng |
| collection | DOAJ |
| description | In this work, a novel electrochemical aptasensor for the detection of ochratoxin A (OTA) was developed using a three-dimensionally ordered macroporous (3DOM) reduced graphene oxide–Au nanoparticles–molybdenum disulfide (RGO-AuNPs-MoS<sub>2</sub>) composite material as the sensing interface and Au nanoparticles–carbon-coated ferroferric oxide (AuNPs-Fe<sub>3</sub>O<sub>4</sub>@C) composite as the signal amplifier. The 3DOM RGO-AuNPs-MoS<sub>2</sub> composite exhibited excellent conductivity and catalytic activity. The 3DOM RGO-AuNPs-MoS<sub>2</sub> film was electrodeposited onto the Au electrode surface to immobilize DNA capture probe (CP), which was then hybridized with DNA helper strands (HS) and OTA aptamer (OPT) to form a Y-shaped structure. In the presence of OTA, the OPT was released from the electrode and then combined with AuNPs-Fe<sub>3</sub>O<sub>4</sub>@C containing the signal DNA1-metheylene blue (S1-MB) and DNA2-metheylene blue (S2-MB). The current response coming from MB was proportional with the OTA concentration. Under optimal conditions, the linear range of the aptasensor was 1 fg/mL to 0.1 μg/mL, with a detection limit as low as 0.56 fg/mL. The aptasensor was also used to detect OTA in rice and wheat samples, and the results were in agreement with those obtained by liquid chromatography-mass spectrometry (HPLC-MS). |
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| language | English |
| last_indexed | 2024-03-11T01:12:24Z |
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| spelling | doaj.art-2cc789d578a543df97b4ead02248ec3b2023-11-18T18:44:33ZengMDPI AGCatalysts2073-43442023-07-01137108810.3390/catal13071088A Novel Ochratoxin A Aptasensor Based on Three-Dimensionally Ordered Macroporous RGO-AuNPs-MoS<sub>2</sub> Enhanced Electrocatalysis of Methylene Blue and AuNPs-Fe<sub>3</sub>O<sub>4</sub>@C Composite as Signal Probe CarrierGang Peng0Xu Zhang1Kaiwen Zhang2Xiaojun Chen3He Huang4College of Food Engineering, Anhui Science and Technology University, Chuzhou 233100, ChinaCollege of Food Engineering, Anhui Science and Technology University, Chuzhou 233100, ChinaCollege of Food Engineering, Anhui Science and Technology University, Chuzhou 233100, ChinaCollege of Chemistry and Molecular Engineering, Nanjing Tech University, Nanjing 211816, ChinaCollege of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, ChinaIn this work, a novel electrochemical aptasensor for the detection of ochratoxin A (OTA) was developed using a three-dimensionally ordered macroporous (3DOM) reduced graphene oxide–Au nanoparticles–molybdenum disulfide (RGO-AuNPs-MoS<sub>2</sub>) composite material as the sensing interface and Au nanoparticles–carbon-coated ferroferric oxide (AuNPs-Fe<sub>3</sub>O<sub>4</sub>@C) composite as the signal amplifier. The 3DOM RGO-AuNPs-MoS<sub>2</sub> composite exhibited excellent conductivity and catalytic activity. The 3DOM RGO-AuNPs-MoS<sub>2</sub> film was electrodeposited onto the Au electrode surface to immobilize DNA capture probe (CP), which was then hybridized with DNA helper strands (HS) and OTA aptamer (OPT) to form a Y-shaped structure. In the presence of OTA, the OPT was released from the electrode and then combined with AuNPs-Fe<sub>3</sub>O<sub>4</sub>@C containing the signal DNA1-metheylene blue (S1-MB) and DNA2-metheylene blue (S2-MB). The current response coming from MB was proportional with the OTA concentration. Under optimal conditions, the linear range of the aptasensor was 1 fg/mL to 0.1 μg/mL, with a detection limit as low as 0.56 fg/mL. The aptasensor was also used to detect OTA in rice and wheat samples, and the results were in agreement with those obtained by liquid chromatography-mass spectrometry (HPLC-MS).https://www.mdpi.com/2073-4344/13/7/1088aptasensorochratoxin Athree dimensionally ordered microporousMoS<sub>2</sub>RGO |
| spellingShingle | Gang Peng Xu Zhang Kaiwen Zhang Xiaojun Chen He Huang A Novel Ochratoxin A Aptasensor Based on Three-Dimensionally Ordered Macroporous RGO-AuNPs-MoS<sub>2</sub> Enhanced Electrocatalysis of Methylene Blue and AuNPs-Fe<sub>3</sub>O<sub>4</sub>@C Composite as Signal Probe Carrier Catalysts aptasensor ochratoxin A three dimensionally ordered microporous MoS<sub>2</sub> RGO |
| title | A Novel Ochratoxin A Aptasensor Based on Three-Dimensionally Ordered Macroporous RGO-AuNPs-MoS<sub>2</sub> Enhanced Electrocatalysis of Methylene Blue and AuNPs-Fe<sub>3</sub>O<sub>4</sub>@C Composite as Signal Probe Carrier |
| title_full | A Novel Ochratoxin A Aptasensor Based on Three-Dimensionally Ordered Macroporous RGO-AuNPs-MoS<sub>2</sub> Enhanced Electrocatalysis of Methylene Blue and AuNPs-Fe<sub>3</sub>O<sub>4</sub>@C Composite as Signal Probe Carrier |
| title_fullStr | A Novel Ochratoxin A Aptasensor Based on Three-Dimensionally Ordered Macroporous RGO-AuNPs-MoS<sub>2</sub> Enhanced Electrocatalysis of Methylene Blue and AuNPs-Fe<sub>3</sub>O<sub>4</sub>@C Composite as Signal Probe Carrier |
| title_full_unstemmed | A Novel Ochratoxin A Aptasensor Based on Three-Dimensionally Ordered Macroporous RGO-AuNPs-MoS<sub>2</sub> Enhanced Electrocatalysis of Methylene Blue and AuNPs-Fe<sub>3</sub>O<sub>4</sub>@C Composite as Signal Probe Carrier |
| title_short | A Novel Ochratoxin A Aptasensor Based on Three-Dimensionally Ordered Macroporous RGO-AuNPs-MoS<sub>2</sub> Enhanced Electrocatalysis of Methylene Blue and AuNPs-Fe<sub>3</sub>O<sub>4</sub>@C Composite as Signal Probe Carrier |
| title_sort | novel ochratoxin a aptasensor based on three dimensionally ordered macroporous rgo aunps mos sub 2 sub enhanced electrocatalysis of methylene blue and aunps fe sub 3 sub o sub 4 sub c composite as signal probe carrier |
| topic | aptasensor ochratoxin A three dimensionally ordered microporous MoS<sub>2</sub> RGO |
| url | https://www.mdpi.com/2073-4344/13/7/1088 |
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