An Improved Protocol for the Production of Lentiviral Vectors

Summary: Lentiviral vectors are an ideal gene-delivery system for large gene-editing tools, such as the clustered regularly interspaced short palindromic repeat (CRISPR)-Cas9 system, due to their high packaging capacity and broad tropism. Here, we present a calcium phosphate-based protocol for lenti...

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Bibliographic Details
Main Authors: Logan Y. Brown, Wendy Dong, Boris Kantor
Format: Article
Language:English
Published: Elsevier 2020-12-01
Series:STAR Protocols
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166720301398
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Summary:Summary: Lentiviral vectors are an ideal gene-delivery system for large gene-editing tools, such as the clustered regularly interspaced short palindromic repeat (CRISPR)-Cas9 system, due to their high packaging capacity and broad tropism. Here, we present a calcium phosphate-based protocol for lentiviral production and concentration for in vitro and in vivo use. This revised procedure has been optimized to ensure high viral titers and transduction efficiency and is scalable to meet specific production needs.
ISSN:2666-1667