Quantification of the Ability of Natural Products to Prevent Herpes Virus Infection
<b>Background:</b> Herpes simplex virus (HSV) is usually dormant and becomes apparent when body conditions decline. We investigated the anti-HSV activity of various natural and synthetic compounds for future clinical application. <b>Methods:</b> Mock- and HSV-infected Vero ce...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-10-01
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| Series: | Medicines |
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| Online Access: | https://www.mdpi.com/2305-6320/7/10/64 |
| _version_ | 1797551677435281408 |
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| author | Kunihiko Fukuchi Hiroshi Sakagami Yoshiaki Sugita Koichi Takao Daisuke Asai Shigemi Terakubo Hiromu Takemura Hirokazu Ohno Misaki Horiuchi Madoka Suguro Tomohiro Fujisawa Kazuki Toeda Hiroshi Oizumi Toshikazu Yasui Takaaki Oizumi |
| author_facet | Kunihiko Fukuchi Hiroshi Sakagami Yoshiaki Sugita Koichi Takao Daisuke Asai Shigemi Terakubo Hiromu Takemura Hirokazu Ohno Misaki Horiuchi Madoka Suguro Tomohiro Fujisawa Kazuki Toeda Hiroshi Oizumi Toshikazu Yasui Takaaki Oizumi |
| author_sort | Kunihiko Fukuchi |
| collection | DOAJ |
| description | <b>Background:</b> Herpes simplex virus (HSV) is usually dormant and becomes apparent when body conditions decline. We investigated the anti-HSV activity of various natural and synthetic compounds for future clinical application. <b>Methods:</b> Mock- and HSV-infected Vero cells were treated for three days with various concentrations of samples. For short exposure, 100-fold concentrated virus were preincubated for 3 min with samples, diluted to normal multiplicity of infection (MOI), before the addition to the cells. Anti-HSV activity was evaluated by the chemotherapy index. <b>Results:</b> Alkaline extracts of the leaves of <i>Sasa</i> sp. (SE) and pine cone (PCE) showed higher anti-HSV activity than 20 Japanese traditional herb medicines (Kampo formulas), four popular polyphenols, and 119 chromone-related compounds. Exposure of HSV to SE or PCE for 3 min almost completely eliminated the infectivity of HSV, whereas much longer exposure time was required for Kakkonto, the most active Kampo formulae. Anti-HSV activity of PCE and Kakkonto could be detected only when they were dissolved by alkaline solution (pH 8.0), but not by neutral buffer (pH 7.4). Anti-HSV activity of SE and povidone iodine was stable if they were diluted with neutral buffer. <b>Conclusions:</b> The present study suggests the applicability of SE and PCE for treatment of oral HSV and possibly other viruses. |
| first_indexed | 2024-03-10T15:49:29Z |
| format | Article |
| id | doaj.art-2ccacfdf62f0473895fd7b384b36ca49 |
| institution | Directory Open Access Journal |
| issn | 2305-6320 |
| language | English |
| last_indexed | 2024-03-10T15:49:29Z |
| publishDate | 2020-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Medicines |
| spelling | doaj.art-2ccacfdf62f0473895fd7b384b36ca492023-11-20T16:09:33ZengMDPI AGMedicines2305-63202020-10-017106410.3390/medicines7100064Quantification of the Ability of Natural Products to Prevent Herpes Virus InfectionKunihiko Fukuchi0Hiroshi Sakagami1Yoshiaki Sugita2Koichi Takao3Daisuke Asai4Shigemi Terakubo5Hiromu Takemura6Hirokazu Ohno7Misaki Horiuchi8Madoka Suguro9Tomohiro Fujisawa10Kazuki Toeda11Hiroshi Oizumi12Toshikazu Yasui13Takaaki Oizumi14Graduate School of Health Sciences, Showa University, Hatanodai 1-5-8, Shinagawa, Tokyo 142-8555, JapanResearch Institute of Odontology (M-RIO), Meikai University, Keyakidai 1-1, Sakado, Saitama 350-0283, JapanDepartment of Pharmaceutical Sciences, Faculty of Pharmacy and Pharmaceutical Sciences, Josai University, Keyakidai 1-1, Sakado, Saitama 350-0295, JapanDepartment of Pharmaceutical Sciences, Faculty of Pharmacy and Pharmaceutical Sciences, Josai University, Keyakidai 1-1, Sakado, Saitama 350-0295, JapanDepartment of Microbiology, St. Marianna University School of Medicine, Sugao 2-16-1, Miyamae, Kawasaki 216-8511, JapanDepartment of Microbiology, St. Marianna University School of Medicine, Sugao 2-16-1, Miyamae, Kawasaki 216-8511, JapanDepartment of Microbiology, St. Marianna University School of Medicine, Sugao 2-16-1, Miyamae, Kawasaki 216-8511, JapanMaruzen Pharmaceuticals Co., Ltd., Fukuyama, Hiroshima 729-3103, JapanDaiwa Biological Research Institute Co., Ltd., Sakado 3-2-1, Takatsu-ku, Kawasaki, Kanagawa 213-0012, JapanDaiwa Biological Research Institute Co., Ltd., Sakado 3-2-1, Takatsu-ku, Kawasaki, Kanagawa 213-0012, JapanDaiwa Biological Research Institute Co., Ltd., Sakado 3-2-1, Takatsu-ku, Kawasaki, Kanagawa 213-0012, JapanDaiwa Biological Research Institute Co., Ltd., Sakado 3-2-1, Takatsu-ku, Kawasaki, Kanagawa 213-0012, JapanDaiwa Biological Research Institute Co., Ltd., Sakado 3-2-1, Takatsu-ku, Kawasaki, Kanagawa 213-0012, JapanDivision of Oral Health, School of Dentistry, Meikai University, Keyakidai 1-1, Sakado, Saitama 350-0283, JapanDaiwa Biological Research Institute Co., Ltd., Sakado 3-2-1, Takatsu-ku, Kawasaki, Kanagawa 213-0012, Japan<b>Background:</b> Herpes simplex virus (HSV) is usually dormant and becomes apparent when body conditions decline. We investigated the anti-HSV activity of various natural and synthetic compounds for future clinical application. <b>Methods:</b> Mock- and HSV-infected Vero cells were treated for three days with various concentrations of samples. For short exposure, 100-fold concentrated virus were preincubated for 3 min with samples, diluted to normal multiplicity of infection (MOI), before the addition to the cells. Anti-HSV activity was evaluated by the chemotherapy index. <b>Results:</b> Alkaline extracts of the leaves of <i>Sasa</i> sp. (SE) and pine cone (PCE) showed higher anti-HSV activity than 20 Japanese traditional herb medicines (Kampo formulas), four popular polyphenols, and 119 chromone-related compounds. Exposure of HSV to SE or PCE for 3 min almost completely eliminated the infectivity of HSV, whereas much longer exposure time was required for Kakkonto, the most active Kampo formulae. Anti-HSV activity of PCE and Kakkonto could be detected only when they were dissolved by alkaline solution (pH 8.0), but not by neutral buffer (pH 7.4). Anti-HSV activity of SE and povidone iodine was stable if they were diluted with neutral buffer. <b>Conclusions:</b> The present study suggests the applicability of SE and PCE for treatment of oral HSV and possibly other viruses.https://www.mdpi.com/2305-6320/7/10/64Kampo formulaealkaline extract of <i>Sasa</i> sp.pine cone extractpovidone-iodineHSVHIV |
| spellingShingle | Kunihiko Fukuchi Hiroshi Sakagami Yoshiaki Sugita Koichi Takao Daisuke Asai Shigemi Terakubo Hiromu Takemura Hirokazu Ohno Misaki Horiuchi Madoka Suguro Tomohiro Fujisawa Kazuki Toeda Hiroshi Oizumi Toshikazu Yasui Takaaki Oizumi Quantification of the Ability of Natural Products to Prevent Herpes Virus Infection Medicines Kampo formulae alkaline extract of <i>Sasa</i> sp. pine cone extract povidone-iodine HSV HIV |
| title | Quantification of the Ability of Natural Products to Prevent Herpes Virus Infection |
| title_full | Quantification of the Ability of Natural Products to Prevent Herpes Virus Infection |
| title_fullStr | Quantification of the Ability of Natural Products to Prevent Herpes Virus Infection |
| title_full_unstemmed | Quantification of the Ability of Natural Products to Prevent Herpes Virus Infection |
| title_short | Quantification of the Ability of Natural Products to Prevent Herpes Virus Infection |
| title_sort | quantification of the ability of natural products to prevent herpes virus infection |
| topic | Kampo formulae alkaline extract of <i>Sasa</i> sp. pine cone extract povidone-iodine HSV HIV |
| url | https://www.mdpi.com/2305-6320/7/10/64 |
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