Isolation and characterization of a MADS-box gene in cucumber (Cucumis sativus L.) that affects flowering time and leaf morphology in transgenic Arabidopsis
The MADS-box family genes are important transcription factors that play essential roles in plant growth and development. In this study, a MADS-box gene, CsMADS02, was identified and functionally characterized in cucumber (Cucumis sativus L.). CsMADS02 encoded a typical SEP MADS-box protein which con...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2019-01-01
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| Series: | Biotechnology & Biotechnological Equipment |
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| Online Access: | http://dx.doi.org/10.1080/13102818.2018.1534556 |
| _version_ | 1818906598664306688 |
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| author | Yong Zhou Lifang Hu Jianbo Song Lunwei Jiang Shiqiang Liu |
| author_facet | Yong Zhou Lifang Hu Jianbo Song Lunwei Jiang Shiqiang Liu |
| author_sort | Yong Zhou |
| collection | DOAJ |
| description | The MADS-box family genes are important transcription factors that play essential roles in plant growth and development. In this study, a MADS-box gene, CsMADS02, was identified and functionally characterized in cucumber (Cucumis sativus L.). CsMADS02 encoded a typical SEP MADS-box protein which contained a MADS-box, a K-box, an I region and a C region, as well as two short conserved SEP motifs. Sequence alignment and phylogenetic analysis showed that the CsMADS02 protein belonged to the SEP3 subfamily and was highly homologous to other published plant SEP proteins. Expression analyses revealed that CsMADS02 was primarily expressed in flowers and ovaries, and differentially expressed during the development of male and female flowers. Ectopic overexpression of CsMADS02 caused earlier flowering of Arabidopsis. In addition, morphological changes were also observed in transgenic plants, including curled and fewer rosette leaves. These findings suggest that CsMADS02 is a potential regulator of the flowering time and leaf morphology in cucumber. |
| first_indexed | 2024-12-19T21:41:47Z |
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| id | doaj.art-2ccaf9b1fe8d477fb3952125c2903d07 |
| institution | Directory Open Access Journal |
| issn | 1310-2818 1314-3530 |
| language | English |
| last_indexed | 2024-12-19T21:41:47Z |
| publishDate | 2019-01-01 |
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| series | Biotechnology & Biotechnological Equipment |
| spelling | doaj.art-2ccaf9b1fe8d477fb3952125c2903d072022-12-21T20:04:38ZengTaylor & Francis GroupBiotechnology & Biotechnological Equipment1310-28181314-35302019-01-01331546310.1080/13102818.2018.15345561534556Isolation and characterization of a MADS-box gene in cucumber (Cucumis sativus L.) that affects flowering time and leaf morphology in transgenic ArabidopsisYong Zhou0Lifang Hu1Jianbo Song2Lunwei Jiang3Shiqiang Liu4Jiangxi Agricultural UniversityJiangxi Agricultural UniversityJiangxi Agricultural UniversityJiangxi Agricultural UniversityJiangxi Agricultural UniversityThe MADS-box family genes are important transcription factors that play essential roles in plant growth and development. In this study, a MADS-box gene, CsMADS02, was identified and functionally characterized in cucumber (Cucumis sativus L.). CsMADS02 encoded a typical SEP MADS-box protein which contained a MADS-box, a K-box, an I region and a C region, as well as two short conserved SEP motifs. Sequence alignment and phylogenetic analysis showed that the CsMADS02 protein belonged to the SEP3 subfamily and was highly homologous to other published plant SEP proteins. Expression analyses revealed that CsMADS02 was primarily expressed in flowers and ovaries, and differentially expressed during the development of male and female flowers. Ectopic overexpression of CsMADS02 caused earlier flowering of Arabidopsis. In addition, morphological changes were also observed in transgenic plants, including curled and fewer rosette leaves. These findings suggest that CsMADS02 is a potential regulator of the flowering time and leaf morphology in cucumber.http://dx.doi.org/10.1080/13102818.2018.1534556cucumbermads-boxsepallata (sep)flowering timetransgenic arabidopsis |
| spellingShingle | Yong Zhou Lifang Hu Jianbo Song Lunwei Jiang Shiqiang Liu Isolation and characterization of a MADS-box gene in cucumber (Cucumis sativus L.) that affects flowering time and leaf morphology in transgenic Arabidopsis Biotechnology & Biotechnological Equipment cucumber mads-box sepallata (sep) flowering time transgenic arabidopsis |
| title | Isolation and characterization of a MADS-box gene in cucumber (Cucumis sativus L.) that affects flowering time and leaf morphology in transgenic Arabidopsis |
| title_full | Isolation and characterization of a MADS-box gene in cucumber (Cucumis sativus L.) that affects flowering time and leaf morphology in transgenic Arabidopsis |
| title_fullStr | Isolation and characterization of a MADS-box gene in cucumber (Cucumis sativus L.) that affects flowering time and leaf morphology in transgenic Arabidopsis |
| title_full_unstemmed | Isolation and characterization of a MADS-box gene in cucumber (Cucumis sativus L.) that affects flowering time and leaf morphology in transgenic Arabidopsis |
| title_short | Isolation and characterization of a MADS-box gene in cucumber (Cucumis sativus L.) that affects flowering time and leaf morphology in transgenic Arabidopsis |
| title_sort | isolation and characterization of a mads box gene in cucumber cucumis sativus l that affects flowering time and leaf morphology in transgenic arabidopsis |
| topic | cucumber mads-box sepallata (sep) flowering time transgenic arabidopsis |
| url | http://dx.doi.org/10.1080/13102818.2018.1534556 |
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