Hydrophilic nanofibers with aligned topography modulate macrophage-mediated host responses via the NLRP3 inflammasome
Abstract Successful biomaterial implantation requires appropriate immune responses. Macrophages are key mediators involved in this process. Currently, exploitation of the intrinsic properties of biomaterials to modulate macrophages and immune responses is appealing. In this study, we prepared hydrop...
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| Format: | Article |
| Language: | English |
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BMC
2023-08-01
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| Series: | Journal of Nanobiotechnology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12951-023-02024-9 |
| _version_ | 1797556703968886784 |
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| author | Yiming Ren Yi Chen Wei Chen Haotian Deng Peiqi Li Yubo Liu Cangjian Gao Guangzhao Tian Chao Ning Zhiguo Yuan Xiang Sui Shuyun Liu Quanyi Guo |
| author_facet | Yiming Ren Yi Chen Wei Chen Haotian Deng Peiqi Li Yubo Liu Cangjian Gao Guangzhao Tian Chao Ning Zhiguo Yuan Xiang Sui Shuyun Liu Quanyi Guo |
| author_sort | Yiming Ren |
| collection | DOAJ |
| description | Abstract Successful biomaterial implantation requires appropriate immune responses. Macrophages are key mediators involved in this process. Currently, exploitation of the intrinsic properties of biomaterials to modulate macrophages and immune responses is appealing. In this study, we prepared hydrophilic nanofibers with an aligned topography by incorporating polyethylene glycol and polycaprolactone using axial electrospinning. We investigated the effect of the nanofibers on macrophage behavior and the underlying mechanisms. With the increase of hydrophilicity of aligned nanofibers, the inflammatory gene expression of macrophages adhering to them was downregulated, and M2 polarization was induced. We further presented clear evidence that the inflammasome NOD-like receptor thermal protein domain associated protein 3 (NLRP3) was the cellular sensor by which macrophages sense the biomaterials, and it acted as a regulator of the macrophage-mediated response to foreign bodies and implant integration. In vivo, we showed that the fibers shaped the implant-related immune microenvironment and ameliorated peritendinous adhesions. In conclusion, our study demonstrated that hydrophilic aligned nanofibers exhibited better biocompatibility and immunological properties. |
| first_indexed | 2024-03-10T17:05:41Z |
| format | Article |
| id | doaj.art-2ccf0965d3754084891b60a621c60380 |
| institution | Directory Open Access Journal |
| issn | 1477-3155 |
| language | English |
| last_indexed | 2024-03-10T17:05:41Z |
| publishDate | 2023-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Nanobiotechnology |
| spelling | doaj.art-2ccf0965d3754084891b60a621c603802023-11-20T10:48:11ZengBMCJournal of Nanobiotechnology1477-31552023-08-0121111410.1186/s12951-023-02024-9Hydrophilic nanofibers with aligned topography modulate macrophage-mediated host responses via the NLRP3 inflammasomeYiming Ren0Yi Chen1Wei Chen2Haotian Deng3Peiqi Li4Yubo Liu5Cangjian Gao6Guangzhao Tian7Chao Ning8Zhiguo Yuan9Xiang Sui10Shuyun Liu11Quanyi Guo12School of Medicine, Nankai UniversityPlastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical CollegeSchool of Medicine, Nankai UniversitySchool of Medicine, Nankai UniversitySchool of Medicine, Nankai UniversitySchool of Medicine, Nankai UniversityInstitute of Orthopedics, First Medical Center, Beijing Key Laboratory of Regenerative Medicine in Orthopedics, Key Laboratory of Musculoskeletal Trauma and War Injuries PLA, Chinese PLA General HospitalSchool of Medicine, Nankai UniversityInstitute of Orthopedics, First Medical Center, Beijing Key Laboratory of Regenerative Medicine in Orthopedics, Key Laboratory of Musculoskeletal Trauma and War Injuries PLA, Chinese PLA General HospitalInstitute of Orthopedics, First Medical Center, Beijing Key Laboratory of Regenerative Medicine in Orthopedics, Key Laboratory of Musculoskeletal Trauma and War Injuries PLA, Chinese PLA General HospitalInstitute of Orthopedics, First Medical Center, Beijing Key Laboratory of Regenerative Medicine in Orthopedics, Key Laboratory of Musculoskeletal Trauma and War Injuries PLA, Chinese PLA General HospitalSchool of Medicine, Nankai UniversitySchool of Medicine, Nankai UniversityAbstract Successful biomaterial implantation requires appropriate immune responses. Macrophages are key mediators involved in this process. Currently, exploitation of the intrinsic properties of biomaterials to modulate macrophages and immune responses is appealing. In this study, we prepared hydrophilic nanofibers with an aligned topography by incorporating polyethylene glycol and polycaprolactone using axial electrospinning. We investigated the effect of the nanofibers on macrophage behavior and the underlying mechanisms. With the increase of hydrophilicity of aligned nanofibers, the inflammatory gene expression of macrophages adhering to them was downregulated, and M2 polarization was induced. We further presented clear evidence that the inflammasome NOD-like receptor thermal protein domain associated protein 3 (NLRP3) was the cellular sensor by which macrophages sense the biomaterials, and it acted as a regulator of the macrophage-mediated response to foreign bodies and implant integration. In vivo, we showed that the fibers shaped the implant-related immune microenvironment and ameliorated peritendinous adhesions. In conclusion, our study demonstrated that hydrophilic aligned nanofibers exhibited better biocompatibility and immunological properties.https://doi.org/10.1186/s12951-023-02024-9TopographyHydrophilicityMacrophageNLRP3 inflammasomeImmunomodulationPeritendinous adhesion |
| spellingShingle | Yiming Ren Yi Chen Wei Chen Haotian Deng Peiqi Li Yubo Liu Cangjian Gao Guangzhao Tian Chao Ning Zhiguo Yuan Xiang Sui Shuyun Liu Quanyi Guo Hydrophilic nanofibers with aligned topography modulate macrophage-mediated host responses via the NLRP3 inflammasome Journal of Nanobiotechnology Topography Hydrophilicity Macrophage NLRP3 inflammasome Immunomodulation Peritendinous adhesion |
| title | Hydrophilic nanofibers with aligned topography modulate macrophage-mediated host responses via the NLRP3 inflammasome |
| title_full | Hydrophilic nanofibers with aligned topography modulate macrophage-mediated host responses via the NLRP3 inflammasome |
| title_fullStr | Hydrophilic nanofibers with aligned topography modulate macrophage-mediated host responses via the NLRP3 inflammasome |
| title_full_unstemmed | Hydrophilic nanofibers with aligned topography modulate macrophage-mediated host responses via the NLRP3 inflammasome |
| title_short | Hydrophilic nanofibers with aligned topography modulate macrophage-mediated host responses via the NLRP3 inflammasome |
| title_sort | hydrophilic nanofibers with aligned topography modulate macrophage mediated host responses via the nlrp3 inflammasome |
| topic | Topography Hydrophilicity Macrophage NLRP3 inflammasome Immunomodulation Peritendinous adhesion |
| url | https://doi.org/10.1186/s12951-023-02024-9 |
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