Protein Assistants of Small Ribosomal Subunit Biogenesis in Bacteria
Ribosome biogenesis is a fundamental and multistage process. The basic steps of ribosome assembly are the transcription, processing, folding, and modification of rRNA; the translation, folding, and modification of r-proteins; and consecutive binding of ribosomal proteins to rRNAs. Ribosome maturatio...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-03-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/10/4/747 |
| _version_ | 1797434347454726144 |
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| author | Elena Maksimova Olesya Kravchenko Alexey Korepanov Elena Stolboushkina |
| author_facet | Elena Maksimova Olesya Kravchenko Alexey Korepanov Elena Stolboushkina |
| author_sort | Elena Maksimova |
| collection | DOAJ |
| description | Ribosome biogenesis is a fundamental and multistage process. The basic steps of ribosome assembly are the transcription, processing, folding, and modification of rRNA; the translation, folding, and modification of r-proteins; and consecutive binding of ribosomal proteins to rRNAs. Ribosome maturation is facilitated by biogenesis factors that include a broad spectrum of proteins: GTPases, RNA helicases, endonucleases, modification enzymes, molecular chaperones, etc. The ribosome assembly factors assist proper rRNA folding and protein–RNA interactions and may sense the checkpoints during the assembly to ensure correct order of this process. Inactivation of these factors is accompanied by severe growth phenotypes and accumulation of immature ribosomal subunits containing unprocessed rRNA, which reduces overall translation efficiency and causes translational errors. In this review, we focus on the structural and biochemical analysis of the 30S ribosomal subunit assembly factors RbfA, YjeQ (RsgA), Era, KsgA (RsmA), RimJ, RimM, RimP, and Hfq, which take part in the decoding-center folding. |
| first_indexed | 2024-03-09T10:30:55Z |
| format | Article |
| id | doaj.art-2ccf938842c744b39b9b490b8b4d6546 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T10:30:55Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-2ccf938842c744b39b9b490b8b4d65462023-12-01T21:15:04ZengMDPI AGMicroorganisms2076-26072022-03-0110474710.3390/microorganisms10040747Protein Assistants of Small Ribosomal Subunit Biogenesis in BacteriaElena Maksimova0Olesya Kravchenko1Alexey Korepanov2Elena Stolboushkina3Institute of Protein Research, Russian Academy of Sciences, 142290 Pushchino, RussiaInstitute of Protein Research, Russian Academy of Sciences, 142290 Pushchino, RussiaInstitute of Protein Research, Russian Academy of Sciences, 142290 Pushchino, RussiaInstitute of Protein Research, Russian Academy of Sciences, 142290 Pushchino, RussiaRibosome biogenesis is a fundamental and multistage process. The basic steps of ribosome assembly are the transcription, processing, folding, and modification of rRNA; the translation, folding, and modification of r-proteins; and consecutive binding of ribosomal proteins to rRNAs. Ribosome maturation is facilitated by biogenesis factors that include a broad spectrum of proteins: GTPases, RNA helicases, endonucleases, modification enzymes, molecular chaperones, etc. The ribosome assembly factors assist proper rRNA folding and protein–RNA interactions and may sense the checkpoints during the assembly to ensure correct order of this process. Inactivation of these factors is accompanied by severe growth phenotypes and accumulation of immature ribosomal subunits containing unprocessed rRNA, which reduces overall translation efficiency and causes translational errors. In this review, we focus on the structural and biochemical analysis of the 30S ribosomal subunit assembly factors RbfA, YjeQ (RsgA), Era, KsgA (RsmA), RimJ, RimM, RimP, and Hfq, which take part in the decoding-center folding.https://www.mdpi.com/2076-2607/10/4/747ribosome biogenesis30S subunit maturation17S rRNA foldingassembly factorsRbfAYjeQ (RsgA) |
| spellingShingle | Elena Maksimova Olesya Kravchenko Alexey Korepanov Elena Stolboushkina Protein Assistants of Small Ribosomal Subunit Biogenesis in Bacteria Microorganisms ribosome biogenesis 30S subunit maturation 17S rRNA folding assembly factors RbfA YjeQ (RsgA) |
| title | Protein Assistants of Small Ribosomal Subunit Biogenesis in Bacteria |
| title_full | Protein Assistants of Small Ribosomal Subunit Biogenesis in Bacteria |
| title_fullStr | Protein Assistants of Small Ribosomal Subunit Biogenesis in Bacteria |
| title_full_unstemmed | Protein Assistants of Small Ribosomal Subunit Biogenesis in Bacteria |
| title_short | Protein Assistants of Small Ribosomal Subunit Biogenesis in Bacteria |
| title_sort | protein assistants of small ribosomal subunit biogenesis in bacteria |
| topic | ribosome biogenesis 30S subunit maturation 17S rRNA folding assembly factors RbfA YjeQ (RsgA) |
| url | https://www.mdpi.com/2076-2607/10/4/747 |
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