Experimental determination of the pharmacokinetic properties of trimethoprim and sulfamethoxazole combination in the blood serum of broiler chickens
A rapid, simple and highly efficient analytical method for the targeted determination of trimethoprim and sulfamethoxazole in serum samples has been developed and used to measure the pharmacokinetic curve of these medicinal substances after administration to chicken broilers. The pharmacokinetics pr...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Czech Academy of Agricultural Sciences
2021-06-01
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| Series: | Veterinární Medicína |
| Subjects: | |
| Online Access: | https://vetmed.agriculturejournals.cz/artkey/vet-202106-0003_experimental-determination-of-the-pharmacokinetic-properties-of-trimethoprim-and-sulfamethoxazole-combination-i.php |
| _version_ | 1797897425732501504 |
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| author | K Putecova K Nedbalcova I Bartejsova M Zouharova K Matiaskova E Jeklova M Viskova P Zouzelkova M Jerabek K Stastny |
| author_facet | K Putecova K Nedbalcova I Bartejsova M Zouharova K Matiaskova E Jeklova M Viskova P Zouzelkova M Jerabek K Stastny |
| author_sort | K Putecova |
| collection | DOAJ |
| description | A rapid, simple and highly efficient analytical method for the targeted determination of trimethoprim and sulfamethoxazole in serum samples has been developed and used to measure the pharmacokinetic curve of these medicinal substances after administration to chicken broilers. The pharmacokinetics properties of trimethoprim and sulfamethoxazole were investigated in clinically healthy broiler chickens after the single oral administration of the commercial preparation Methoxasol (Eurovet Animal Health, B.V., The Netherlands) at a dose of 0.275 ml/kg b.w. After a single dose drug administration, the chickens were sacrificed by decapitation under general anaesthesia by Isoflurin 1 000 mg/g (Vetpharma AH, Spain) and the blood was collected at precisely defined intervals: 15, 30, 45, 60, 90, 120, 180, 360 and 720 min after the administration. The serum concentrations of amoxicillin were determined using Q Exactive tandem mass spectrometer (Thermo Fisher Scientific, USA) in conjunction with liquid chromatography. The detected pharmacokinetic parameters of trimethoprim after the oral administration were Cmax = 2.1 ± 1.0 µg/ml; Tmax = 1.5 h; t1/2 = 0.88 h; kel = 0.009 3 ± 0.001 1 1/h; AUCt = 2.901 ± 1.4 µg.h/ml; AUC∞ = 2.907 ± 1.5 µg.h/ml; Vd = 2.632 l/kg; Cl = 2.7 l/h. The pharmacokinetic parameters of sulfamethoxazole after the oral administration were Cmax = 47.1 ± 15.3 µg/ml; Tmax = 1 h; t1/2 = 1.92 h; kel = 0.004 6 ± 0.000 3 1/h; AUCt = 89.676 ± 26.9 µg.h/ml; AUC∞ = 94.612 ± 28.4 µg.h/ml; Vd = 0.584 l/kg; Cl = 0.21 l/h. To the best of our knowledge, this is the first pharmacokinetic study of the combination of sulfamethoxazole and trimethoprim in broiler chickens. |
| first_indexed | 2024-04-10T07:57:17Z |
| format | Article |
| id | doaj.art-2cd1aecd53b94306a95fd3826213e5a5 |
| institution | Directory Open Access Journal |
| issn | 0375-8427 1805-9392 |
| language | English |
| last_indexed | 2024-04-10T07:57:17Z |
| publishDate | 2021-06-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Veterinární Medicína |
| spelling | doaj.art-2cd1aecd53b94306a95fd3826213e5a52023-02-23T03:51:05ZengCzech Academy of Agricultural SciencesVeterinární Medicína0375-84271805-93922021-06-0166624825610.17221/190/2020-VETMEDvet-202106-0003Experimental determination of the pharmacokinetic properties of trimethoprim and sulfamethoxazole combination in the blood serum of broiler chickensK Putecova0K Nedbalcova1I Bartejsova2M Zouharova3K Matiaskova4E Jeklova5M Viskova6P Zouzelkova7M Jerabek8K Stastny9Veterinary Research Institute, Brno, Czech RepublicVeterinary Research Institute, Brno, Czech RepublicVeterinary Research Institute, Brno, Czech RepublicVeterinary Research Institute, Brno, Czech RepublicVeterinary Research Institute, Brno, Czech RepublicVeterinary Research Institute, Brno, Czech RepublicTekro spol. s r.o., Prague, Czech RepublicTekro spol. s r.o., Prague, Czech RepublicTekro spol. s r.o., Prague, Czech RepublicVeterinary Research Institute, Brno, Czech RepublicA rapid, simple and highly efficient analytical method for the targeted determination of trimethoprim and sulfamethoxazole in serum samples has been developed and used to measure the pharmacokinetic curve of these medicinal substances after administration to chicken broilers. The pharmacokinetics properties of trimethoprim and sulfamethoxazole were investigated in clinically healthy broiler chickens after the single oral administration of the commercial preparation Methoxasol (Eurovet Animal Health, B.V., The Netherlands) at a dose of 0.275 ml/kg b.w. After a single dose drug administration, the chickens were sacrificed by decapitation under general anaesthesia by Isoflurin 1 000 mg/g (Vetpharma AH, Spain) and the blood was collected at precisely defined intervals: 15, 30, 45, 60, 90, 120, 180, 360 and 720 min after the administration. The serum concentrations of amoxicillin were determined using Q Exactive tandem mass spectrometer (Thermo Fisher Scientific, USA) in conjunction with liquid chromatography. The detected pharmacokinetic parameters of trimethoprim after the oral administration were Cmax = 2.1 ± 1.0 µg/ml; Tmax = 1.5 h; t1/2 = 0.88 h; kel = 0.009 3 ± 0.001 1 1/h; AUCt = 2.901 ± 1.4 µg.h/ml; AUC∞ = 2.907 ± 1.5 µg.h/ml; Vd = 2.632 l/kg; Cl = 2.7 l/h. The pharmacokinetic parameters of sulfamethoxazole after the oral administration were Cmax = 47.1 ± 15.3 µg/ml; Tmax = 1 h; t1/2 = 1.92 h; kel = 0.004 6 ± 0.000 3 1/h; AUCt = 89.676 ± 26.9 µg.h/ml; AUC∞ = 94.612 ± 28.4 µg.h/ml; Vd = 0.584 l/kg; Cl = 0.21 l/h. To the best of our knowledge, this is the first pharmacokinetic study of the combination of sulfamethoxazole and trimethoprim in broiler chickens.https://vetmed.agriculturejournals.cz/artkey/vet-202106-0003_experimental-determination-of-the-pharmacokinetic-properties-of-trimethoprim-and-sulfamethoxazole-combination-i.phpantimicrobial treatmentdrug concentrationsmass spectrometrypoultrytime dependence |
| spellingShingle | K Putecova K Nedbalcova I Bartejsova M Zouharova K Matiaskova E Jeklova M Viskova P Zouzelkova M Jerabek K Stastny Experimental determination of the pharmacokinetic properties of trimethoprim and sulfamethoxazole combination in the blood serum of broiler chickens Veterinární Medicína antimicrobial treatment drug concentrations mass spectrometry poultry time dependence |
| title | Experimental determination of the pharmacokinetic properties of trimethoprim and sulfamethoxazole combination in the blood serum of broiler chickens |
| title_full | Experimental determination of the pharmacokinetic properties of trimethoprim and sulfamethoxazole combination in the blood serum of broiler chickens |
| title_fullStr | Experimental determination of the pharmacokinetic properties of trimethoprim and sulfamethoxazole combination in the blood serum of broiler chickens |
| title_full_unstemmed | Experimental determination of the pharmacokinetic properties of trimethoprim and sulfamethoxazole combination in the blood serum of broiler chickens |
| title_short | Experimental determination of the pharmacokinetic properties of trimethoprim and sulfamethoxazole combination in the blood serum of broiler chickens |
| title_sort | experimental determination of the pharmacokinetic properties of trimethoprim and sulfamethoxazole combination in the blood serum of broiler chickens |
| topic | antimicrobial treatment drug concentrations mass spectrometry poultry time dependence |
| url | https://vetmed.agriculturejournals.cz/artkey/vet-202106-0003_experimental-determination-of-the-pharmacokinetic-properties-of-trimethoprim-and-sulfamethoxazole-combination-i.php |
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