Lipoic Acid Conjugated Boron Hybrids Enhance Wound Healing and Antimicrobial Processes
Complications of chronic non-healing wounds led to the emergence of nanotechnology-based therapies to enhance healing, facilitate tissue repair, and prevent wound-related complications like infections. Here, we design alpha lipoic acid (ALA) conjugated hexagonal boron nitride (hBN) and boron carbide...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/15/1/149 |
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| author | Hasan Türkez Özge Çağlar Yıldırım Sena Öner Abdurrahim Kadı Abdulkadir Mete Mehmet Enes Arslan İrfan Oğuz Şahin Ömer Erkan Yapça Adil Mardinoğlu |
| author_facet | Hasan Türkez Özge Çağlar Yıldırım Sena Öner Abdurrahim Kadı Abdulkadir Mete Mehmet Enes Arslan İrfan Oğuz Şahin Ömer Erkan Yapça Adil Mardinoğlu |
| author_sort | Hasan Türkez |
| collection | DOAJ |
| description | Complications of chronic non-healing wounds led to the emergence of nanotechnology-based therapies to enhance healing, facilitate tissue repair, and prevent wound-related complications like infections. Here, we design alpha lipoic acid (ALA) conjugated hexagonal boron nitride (hBN) and boron carbide (B<sub>4</sub>C) nanoparticles (NPs) to enhance wound healing in human dermal fibroblast (HDFa) cell culture and characterize its antimicrobial properties against <i>Staphylococcus aureus</i> (<i>S. aureus</i>, gram positive) and <i>Escherichia coli</i> (<i>E. coli</i>, gram negative) bacterial strains. ALA molecules are integrated onto hBN and C<sub>4</sub>B NPs through esterification procedure, and molecular characterizations are performed by using transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), and UV-vis spectroscopy. Wound healing and antimicrobial properties are investigated via the use of cell viability assays, scratch test, oxidative stress, and antimicrobial activity assays. Based on our analysis, we observe that ALA-conjugated hBN NPs have the highest wound-healing feature and antimicrobial activity compared to ALA-B<sub>4</sub>C. On the other hand, hBN, ALA-B<sub>4</sub>C, and ALA compounds showed promising regenerative and antimicrobial properties. Also, we find that ALA conjugation enhances wound healing and antimicrobial potency of hBN and B<sub>4</sub>C NPs. We conclude that the ALA-hBN conjugate is a potential candidate to stimulate regeneration process for injuries. |
| first_indexed | 2024-03-09T11:27:17Z |
| format | Article |
| id | doaj.art-2cd227c1a9954e08a706fc073d3f43a3 |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-09T11:27:17Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-2cd227c1a9954e08a706fc073d3f43a32023-11-30T23:58:25ZengMDPI AGPharmaceutics1999-49232022-12-0115114910.3390/pharmaceutics15010149Lipoic Acid Conjugated Boron Hybrids Enhance Wound Healing and Antimicrobial ProcessesHasan Türkez0Özge Çağlar Yıldırım1Sena Öner2Abdurrahim Kadı3Abdulkadir Mete4Mehmet Enes Arslan5İrfan Oğuz Şahin6Ömer Erkan Yapça7Adil Mardinoğlu8Department of Medical Biology, Faculty of Medicine, Atatürk University, 25240 Erzurum, TurkeyDepartment of Molecular Biology and Genetics, Erzurum Technical University, 25050 Erzurum, TurkeyDepartment of Molecular Biology and Genetics, Erzurum Technical University, 25050 Erzurum, TurkeyDepartment of Molecular Biology and Genetics, Erzurum Technical University, 25050 Erzurum, TurkeyDepartment of Molecular Biology and Genetics, Erzurum Technical University, 25050 Erzurum, TurkeyDepartment of Molecular Biology and Genetics, Erzurum Technical University, 25050 Erzurum, TurkeyDepartment of Pediatrics, Pediatric Cardiology, Faculty of Medicine, Ondokuz Mayıs University, 55139 Samsun, TurkeyDepartment of Gynecology and Obstetrics, Faculty of Medicine, Atatürk University, 25240 Erzurum, TurkeyScience for Life Laboratory, KTH-Royal Institute of Technology, SE-17121 Stockholm, SwedenComplications of chronic non-healing wounds led to the emergence of nanotechnology-based therapies to enhance healing, facilitate tissue repair, and prevent wound-related complications like infections. Here, we design alpha lipoic acid (ALA) conjugated hexagonal boron nitride (hBN) and boron carbide (B<sub>4</sub>C) nanoparticles (NPs) to enhance wound healing in human dermal fibroblast (HDFa) cell culture and characterize its antimicrobial properties against <i>Staphylococcus aureus</i> (<i>S. aureus</i>, gram positive) and <i>Escherichia coli</i> (<i>E. coli</i>, gram negative) bacterial strains. ALA molecules are integrated onto hBN and C<sub>4</sub>B NPs through esterification procedure, and molecular characterizations are performed by using transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), and UV-vis spectroscopy. Wound healing and antimicrobial properties are investigated via the use of cell viability assays, scratch test, oxidative stress, and antimicrobial activity assays. Based on our analysis, we observe that ALA-conjugated hBN NPs have the highest wound-healing feature and antimicrobial activity compared to ALA-B<sub>4</sub>C. On the other hand, hBN, ALA-B<sub>4</sub>C, and ALA compounds showed promising regenerative and antimicrobial properties. Also, we find that ALA conjugation enhances wound healing and antimicrobial potency of hBN and B<sub>4</sub>C NPs. We conclude that the ALA-hBN conjugate is a potential candidate to stimulate regeneration process for injuries.https://www.mdpi.com/1999-4923/15/1/149wound healingantimicrobial effectnanoparticleslipoic acidboron nitrideboron carbide |
| spellingShingle | Hasan Türkez Özge Çağlar Yıldırım Sena Öner Abdurrahim Kadı Abdulkadir Mete Mehmet Enes Arslan İrfan Oğuz Şahin Ömer Erkan Yapça Adil Mardinoğlu Lipoic Acid Conjugated Boron Hybrids Enhance Wound Healing and Antimicrobial Processes Pharmaceutics wound healing antimicrobial effect nanoparticles lipoic acid boron nitride boron carbide |
| title | Lipoic Acid Conjugated Boron Hybrids Enhance Wound Healing and Antimicrobial Processes |
| title_full | Lipoic Acid Conjugated Boron Hybrids Enhance Wound Healing and Antimicrobial Processes |
| title_fullStr | Lipoic Acid Conjugated Boron Hybrids Enhance Wound Healing and Antimicrobial Processes |
| title_full_unstemmed | Lipoic Acid Conjugated Boron Hybrids Enhance Wound Healing and Antimicrobial Processes |
| title_short | Lipoic Acid Conjugated Boron Hybrids Enhance Wound Healing and Antimicrobial Processes |
| title_sort | lipoic acid conjugated boron hybrids enhance wound healing and antimicrobial processes |
| topic | wound healing antimicrobial effect nanoparticles lipoic acid boron nitride boron carbide |
| url | https://www.mdpi.com/1999-4923/15/1/149 |
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