Physicochemical Properties and Biological Activities of Silver Carp Scale Peptide and Its Nanofiltration Fractions
To explore the physicochemical properties and biological functions of silver carp scale peptide (SCSP), its molecular-weight fractions SCSP-I, II, and III obtained by nanofiltration were assessed for their solubility, emulsibility, free radical scavenging ability, effect on the proliferation of mous...
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Frontiers Media S.A.
2022-01-01
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| Series: | Frontiers in Nutrition |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnut.2021.812443/full |
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| author | Xiao-yan Zu Ya-jing Zhao Ya-jing Zhao Shi-ming Fu Tao Liao Hai-lan Li Guang-quan Xiong |
| author_facet | Xiao-yan Zu Ya-jing Zhao Ya-jing Zhao Shi-ming Fu Tao Liao Hai-lan Li Guang-quan Xiong |
| author_sort | Xiao-yan Zu |
| collection | DOAJ |
| description | To explore the physicochemical properties and biological functions of silver carp scale peptide (SCSP), its molecular-weight fractions SCSP-I, II, and III obtained by nanofiltration were assessed for their solubility, emulsibility, free radical scavenging ability, effect on the proliferation of mouse B16 cells. The results showed that the solubility of each fraction of SCSP was higher than 90%, SCSP-II and III were higher than 95%. The antioxidant powers on ⦁OH, O2-⦁ and Fe3+ were ranked as SCSP-III > SCSP-II > SCSP-I > SCSP. All fractions of SCSP had no toxic or side effects in mouse B16 melanoma cells experiments in vitro. At a concentration of 0.01 mg/mL, the tyrosinase activity of B16 cells in the SCSP-II fraction was significantly lower than that of the α-arbutin (P < 0.05), at 65.37%. The molecular weight distribution of SCSP was 399–1404 Dalton and 13 peptide sequences were detected. Among them, SCSP-II contained many hydrophobic amino acids, and SCSP-III stood out for combining arginine with hydrophobic amino acids. This may be the reason why the low molecular-weight SCSPs show the strong antioxidant activity and strong tyrosinase inhibition. The work provides a data base for the development of SCSP and increases the possibility of its application. |
| first_indexed | 2024-12-20T22:50:31Z |
| format | Article |
| id | doaj.art-2cd2a2c684f2410ca94759470886a819 |
| institution | Directory Open Access Journal |
| issn | 2296-861X |
| language | English |
| last_indexed | 2024-12-20T22:50:31Z |
| publishDate | 2022-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Nutrition |
| spelling | doaj.art-2cd2a2c684f2410ca94759470886a8192022-12-21T19:24:15ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2022-01-01810.3389/fnut.2021.812443812443Physicochemical Properties and Biological Activities of Silver Carp Scale Peptide and Its Nanofiltration FractionsXiao-yan Zu0Ya-jing Zhao1Ya-jing Zhao2Shi-ming Fu3Tao Liao4Hai-lan Li5Guang-quan Xiong6Institute of Agricultural Products Processing and Nuclear Agricultural Technology, Hubei Academy of Agricultural Sciences, Wuhan, ChinaInstitute of Agricultural Products Processing and Nuclear Agricultural Technology, Hubei Academy of Agricultural Sciences, Wuhan, ChinaCollege of Petrochemical, Lanzhou University of Technology, Lanzhou, ChinaInstitute of Agricultural Products Processing and Nuclear Agricultural Technology, Hubei Academy of Agricultural Sciences, Wuhan, ChinaInstitute of Agricultural Products Processing and Nuclear Agricultural Technology, Hubei Academy of Agricultural Sciences, Wuhan, ChinaInstitute of Agricultural Products Processing and Nuclear Agricultural Technology, Hubei Academy of Agricultural Sciences, Wuhan, ChinaInstitute of Agricultural Products Processing and Nuclear Agricultural Technology, Hubei Academy of Agricultural Sciences, Wuhan, ChinaTo explore the physicochemical properties and biological functions of silver carp scale peptide (SCSP), its molecular-weight fractions SCSP-I, II, and III obtained by nanofiltration were assessed for their solubility, emulsibility, free radical scavenging ability, effect on the proliferation of mouse B16 cells. The results showed that the solubility of each fraction of SCSP was higher than 90%, SCSP-II and III were higher than 95%. The antioxidant powers on ⦁OH, O2-⦁ and Fe3+ were ranked as SCSP-III > SCSP-II > SCSP-I > SCSP. All fractions of SCSP had no toxic or side effects in mouse B16 melanoma cells experiments in vitro. At a concentration of 0.01 mg/mL, the tyrosinase activity of B16 cells in the SCSP-II fraction was significantly lower than that of the α-arbutin (P < 0.05), at 65.37%. The molecular weight distribution of SCSP was 399–1404 Dalton and 13 peptide sequences were detected. Among them, SCSP-II contained many hydrophobic amino acids, and SCSP-III stood out for combining arginine with hydrophobic amino acids. This may be the reason why the low molecular-weight SCSPs show the strong antioxidant activity and strong tyrosinase inhibition. The work provides a data base for the development of SCSP and increases the possibility of its application.https://www.frontiersin.org/articles/10.3389/fnut.2021.812443/fullsilver carp scale peptidephysicochemical propertiesfree radical scavengingtyrosinase inhibitionamino acid sequence |
| spellingShingle | Xiao-yan Zu Ya-jing Zhao Ya-jing Zhao Shi-ming Fu Tao Liao Hai-lan Li Guang-quan Xiong Physicochemical Properties and Biological Activities of Silver Carp Scale Peptide and Its Nanofiltration Fractions Frontiers in Nutrition silver carp scale peptide physicochemical properties free radical scavenging tyrosinase inhibition amino acid sequence |
| title | Physicochemical Properties and Biological Activities of Silver Carp Scale Peptide and Its Nanofiltration Fractions |
| title_full | Physicochemical Properties and Biological Activities of Silver Carp Scale Peptide and Its Nanofiltration Fractions |
| title_fullStr | Physicochemical Properties and Biological Activities of Silver Carp Scale Peptide and Its Nanofiltration Fractions |
| title_full_unstemmed | Physicochemical Properties and Biological Activities of Silver Carp Scale Peptide and Its Nanofiltration Fractions |
| title_short | Physicochemical Properties and Biological Activities of Silver Carp Scale Peptide and Its Nanofiltration Fractions |
| title_sort | physicochemical properties and biological activities of silver carp scale peptide and its nanofiltration fractions |
| topic | silver carp scale peptide physicochemical properties free radical scavenging tyrosinase inhibition amino acid sequence |
| url | https://www.frontiersin.org/articles/10.3389/fnut.2021.812443/full |
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