MicroRNA-155 targets SOCS1 to inhibit osteoclast differentiation during orthodontic tooth movement
Abstract Background MicroRNA-155 (miR-155) is a multifunctional miRNA whose expression is known to be involved in a range of physiological and pathological processes. Its association with several oral diseases has been established. However, the specific role of miR-155 in orthodontic tooth movement...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-12-01
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| Series: | BMC Oral Health |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12903-023-03443-8 |
| _version_ | 1797413774761656320 |
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| author | Yao Jiao Sicong Mi Xiaoyan Li Yitong Liu Nannan Han Junji Xu Yi Liu Song Li Lijia Guo |
| author_facet | Yao Jiao Sicong Mi Xiaoyan Li Yitong Liu Nannan Han Junji Xu Yi Liu Song Li Lijia Guo |
| author_sort | Yao Jiao |
| collection | DOAJ |
| description | Abstract Background MicroRNA-155 (miR-155) is a multifunctional miRNA whose expression is known to be involved in a range of physiological and pathological processes. Its association with several oral diseases has been established. However, the specific role of miR-155 in orthodontic tooth movement remains unclear. In this study, we investigated the impact of miR-155 on osteoclast differentiation and orthodontic tooth movement models, aiming to explore the underlying mechanisms. Methods In this experiment, we utilized various agents including miR-155 mimic, miR-155 inhibitor, as well as non-specific sequences (NC mimic & NC inhibitor) to treat murine BMMNCs. Subsequently, osteoclast induction (OC) was carried out to examine the changes in the differentiation ability of monocytes under different conditions. To assess these changes, we employed RT-PCR, Western blotting, and TRAP staining techniques. For the orthodontic tooth movement model in mice, the subjects were divided into two groups: the NaCl group (injected with saline solution) and the miR-155 inhibitor group (injected with AntagomiR-155). We observed the impact of orthodontic tooth movement using stereoscopic microscopy, micro-CT, and HE staining. Furthermore, we performed RT-PCR and Western blotting analyses on the tissues surrounding the moving teeth. Additionally, we employed TargetScan to predict potential target genes of miR-155. Results During osteoclast induction of BMMNCs, the expression of miR-155 exhibited an inverse correlation with osteoclast-related markers. Overexpression of miR-155 led to a decrease in osteoclast-related indexes, whereas underexpression of miR-155 increased those indexes. In the mouse orthodontic tooth movement model, the rate of tooth movement was enhanced following injection of the miR-155 inhibitor, leading to heightened osteoclast activity. TargetScan analysis identified SOCS1 as a target gene of miR-155. Conclusions Our results suggest that miR-155 functions as an inhibitor of osteoclast differentiation, and it appears to regulate osteoclasts during orthodontic tooth movement. The regulatory mechanism of miR-155 in this process involves the targeting of SOCS1. |
| first_indexed | 2024-03-09T05:22:55Z |
| format | Article |
| id | doaj.art-2cd660bbce214f7a8cb85568020a57bd |
| institution | Directory Open Access Journal |
| issn | 1472-6831 |
| language | English |
| last_indexed | 2024-03-09T05:22:55Z |
| publishDate | 2023-12-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Oral Health |
| spelling | doaj.art-2cd660bbce214f7a8cb85568020a57bd2023-12-03T12:38:45ZengBMCBMC Oral Health1472-68312023-12-0123111410.1186/s12903-023-03443-8MicroRNA-155 targets SOCS1 to inhibit osteoclast differentiation during orthodontic tooth movementYao Jiao0Sicong Mi1Xiaoyan Li2Yitong Liu3Nannan Han4Junji Xu5Yi Liu6Song Li7Lijia Guo8Department of Periodontics, School of Stomatology, Capital Medical UniversityDepartment of Stomatology, Air Force Medical Center, PLA, The Fourth Military Medical UniversityDepartment of Periodontics, School of Stomatology, Capital Medical UniversityDepartment of Periodontics, School of Stomatology, Capital Medical UniversityDepartment of Periodontics, School of Stomatology, Capital Medical UniversityDepartment of Periodontics, School of Stomatology, Capital Medical UniversityDepartment of Periodontics, School of Stomatology, Capital Medical UniversityDepartment of Orthodontics, School of Stomatology, Capital Medical UniversityDepartment of Orthodontics (WangFuJing Campus), School of Stomatology, Capital Medical UniversityAbstract Background MicroRNA-155 (miR-155) is a multifunctional miRNA whose expression is known to be involved in a range of physiological and pathological processes. Its association with several oral diseases has been established. However, the specific role of miR-155 in orthodontic tooth movement remains unclear. In this study, we investigated the impact of miR-155 on osteoclast differentiation and orthodontic tooth movement models, aiming to explore the underlying mechanisms. Methods In this experiment, we utilized various agents including miR-155 mimic, miR-155 inhibitor, as well as non-specific sequences (NC mimic & NC inhibitor) to treat murine BMMNCs. Subsequently, osteoclast induction (OC) was carried out to examine the changes in the differentiation ability of monocytes under different conditions. To assess these changes, we employed RT-PCR, Western blotting, and TRAP staining techniques. For the orthodontic tooth movement model in mice, the subjects were divided into two groups: the NaCl group (injected with saline solution) and the miR-155 inhibitor group (injected with AntagomiR-155). We observed the impact of orthodontic tooth movement using stereoscopic microscopy, micro-CT, and HE staining. Furthermore, we performed RT-PCR and Western blotting analyses on the tissues surrounding the moving teeth. Additionally, we employed TargetScan to predict potential target genes of miR-155. Results During osteoclast induction of BMMNCs, the expression of miR-155 exhibited an inverse correlation with osteoclast-related markers. Overexpression of miR-155 led to a decrease in osteoclast-related indexes, whereas underexpression of miR-155 increased those indexes. In the mouse orthodontic tooth movement model, the rate of tooth movement was enhanced following injection of the miR-155 inhibitor, leading to heightened osteoclast activity. TargetScan analysis identified SOCS1 as a target gene of miR-155. Conclusions Our results suggest that miR-155 functions as an inhibitor of osteoclast differentiation, and it appears to regulate osteoclasts during orthodontic tooth movement. The regulatory mechanism of miR-155 in this process involves the targeting of SOCS1.https://doi.org/10.1186/s12903-023-03443-8MicroRNA-155OsteoclastOrthodonticsMonocyte |
| spellingShingle | Yao Jiao Sicong Mi Xiaoyan Li Yitong Liu Nannan Han Junji Xu Yi Liu Song Li Lijia Guo MicroRNA-155 targets SOCS1 to inhibit osteoclast differentiation during orthodontic tooth movement BMC Oral Health MicroRNA-155 Osteoclast Orthodontics Monocyte |
| title | MicroRNA-155 targets SOCS1 to inhibit osteoclast differentiation during orthodontic tooth movement |
| title_full | MicroRNA-155 targets SOCS1 to inhibit osteoclast differentiation during orthodontic tooth movement |
| title_fullStr | MicroRNA-155 targets SOCS1 to inhibit osteoclast differentiation during orthodontic tooth movement |
| title_full_unstemmed | MicroRNA-155 targets SOCS1 to inhibit osteoclast differentiation during orthodontic tooth movement |
| title_short | MicroRNA-155 targets SOCS1 to inhibit osteoclast differentiation during orthodontic tooth movement |
| title_sort | microrna 155 targets socs1 to inhibit osteoclast differentiation during orthodontic tooth movement |
| topic | MicroRNA-155 Osteoclast Orthodontics Monocyte |
| url | https://doi.org/10.1186/s12903-023-03443-8 |
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