High generation of reactive oxygen species from neutrophils in patients with severe COVID-19
Abstract Neutrophilia and an elevated neutrophil:lymphocyte ratio are both characteristic features of severe COVID-19 infection. However, functional neutrophil responses have been poorly investigated in this setting. We utilised a novel PMA-based stimulation assay to determine neutrophil-derived rea...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2022-06-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-13825-7 |
| _version_ | 1811334303942967296 |
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| author | Tonny Veenith Helena Martin Martin Le Breuilly Tony Whitehouse Fang Gao-Smith Niharika Duggal Janet M. Lord Rubina Mian David Sarphie Paul Moss |
| author_facet | Tonny Veenith Helena Martin Martin Le Breuilly Tony Whitehouse Fang Gao-Smith Niharika Duggal Janet M. Lord Rubina Mian David Sarphie Paul Moss |
| author_sort | Tonny Veenith |
| collection | DOAJ |
| description | Abstract Neutrophilia and an elevated neutrophil:lymphocyte ratio are both characteristic features of severe COVID-19 infection. However, functional neutrophil responses have been poorly investigated in this setting. We utilised a novel PMA-based stimulation assay to determine neutrophil-derived reactive oxygen species (ROS) generation in patients with severe COVID-19 infection, non-COVID related sepsis and healthy study participants. ROS production was markedly elevated in COVID-19 patients with median values ninefold higher than in healthy controls and was particularly high in patients on mechanical ventilation. ROS generation correlated strongly with neutrophil count and elevated levels were also seen in patients with non-COVID related sepsis. Relative values, adjusted for neutrophil count, were high in both groups but extreme low or high values were seen in two patients who died shortly after testing, potentially indicating a predictive value for neutrophil function. Our results show that the high levels of neutrophils observed in patients with COVID-19 and sepsis exhibit functional capacity for ROS generation. This may contribute to the clinical features of acute disease and represents a potential novel target for therapeutic intervention. |
| first_indexed | 2024-04-13T17:05:49Z |
| format | Article |
| id | doaj.art-2cd7f35aea034eb6a1e1caa21fcb1c9f |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-13T17:05:49Z |
| publishDate | 2022-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-2cd7f35aea034eb6a1e1caa21fcb1c9f2022-12-22T02:38:28ZengNature PortfolioScientific Reports2045-23222022-06-011211910.1038/s41598-022-13825-7High generation of reactive oxygen species from neutrophils in patients with severe COVID-19Tonny Veenith0Helena Martin1Martin Le Breuilly2Tony Whitehouse3Fang Gao-Smith4Niharika Duggal5Janet M. Lord6Rubina Mian7David Sarphie8Paul Moss9Institute of Inflammation and Ageing, University of BirminghamUniversity Hospitals Birmingham NHS Foundation TrustUniversity Hospitals Birmingham NHS Foundation TrustUniversity Hospitals Birmingham NHS Foundation TrustInstitute of Inflammation and Ageing, University of BirminghamInstitute of Inflammation and Ageing, University of BirminghamInstitute of Inflammation and Ageing, University of BirminghamSeroxo LimitedSeroxo LimitedInstitute of Immunology and Immunotherapy, University of BirminghamAbstract Neutrophilia and an elevated neutrophil:lymphocyte ratio are both characteristic features of severe COVID-19 infection. However, functional neutrophil responses have been poorly investigated in this setting. We utilised a novel PMA-based stimulation assay to determine neutrophil-derived reactive oxygen species (ROS) generation in patients with severe COVID-19 infection, non-COVID related sepsis and healthy study participants. ROS production was markedly elevated in COVID-19 patients with median values ninefold higher than in healthy controls and was particularly high in patients on mechanical ventilation. ROS generation correlated strongly with neutrophil count and elevated levels were also seen in patients with non-COVID related sepsis. Relative values, adjusted for neutrophil count, were high in both groups but extreme low or high values were seen in two patients who died shortly after testing, potentially indicating a predictive value for neutrophil function. Our results show that the high levels of neutrophils observed in patients with COVID-19 and sepsis exhibit functional capacity for ROS generation. This may contribute to the clinical features of acute disease and represents a potential novel target for therapeutic intervention.https://doi.org/10.1038/s41598-022-13825-7 |
| spellingShingle | Tonny Veenith Helena Martin Martin Le Breuilly Tony Whitehouse Fang Gao-Smith Niharika Duggal Janet M. Lord Rubina Mian David Sarphie Paul Moss High generation of reactive oxygen species from neutrophils in patients with severe COVID-19 Scientific Reports |
| title | High generation of reactive oxygen species from neutrophils in patients with severe COVID-19 |
| title_full | High generation of reactive oxygen species from neutrophils in patients with severe COVID-19 |
| title_fullStr | High generation of reactive oxygen species from neutrophils in patients with severe COVID-19 |
| title_full_unstemmed | High generation of reactive oxygen species from neutrophils in patients with severe COVID-19 |
| title_short | High generation of reactive oxygen species from neutrophils in patients with severe COVID-19 |
| title_sort | high generation of reactive oxygen species from neutrophils in patients with severe covid 19 |
| url | https://doi.org/10.1038/s41598-022-13825-7 |
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