Transcriptome analysis of heat resistance regulated by quorum sensing system in Glaesserella parasuis
The ability of bacteria to resist heat shock allows them to adapt to different environments. In addition, heat shock resistance is known for their virulence. Our previous study showed that the AI-2/luxS quorum sensing system affects the growth characteristics, biofilm formation, and virulence of Gla...
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Frontiers Media S.A.
2022-08-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.968460/full |
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| author | Bingzhou Zhang Bingzhou Zhang Changsheng Jiang Changsheng Jiang Hua Cao Hua Cao Wei Zeng Wei Zeng Jingping Ren Jingping Ren Yaofang Hu Yaofang Hu Wentao Li Wentao Li Wentao Li Qigai He Qigai He |
| author_facet | Bingzhou Zhang Bingzhou Zhang Changsheng Jiang Changsheng Jiang Hua Cao Hua Cao Wei Zeng Wei Zeng Jingping Ren Jingping Ren Yaofang Hu Yaofang Hu Wentao Li Wentao Li Wentao Li Qigai He Qigai He |
| author_sort | Bingzhou Zhang |
| collection | DOAJ |
| description | The ability of bacteria to resist heat shock allows them to adapt to different environments. In addition, heat shock resistance is known for their virulence. Our previous study showed that the AI-2/luxS quorum sensing system affects the growth characteristics, biofilm formation, and virulence of Glaesserella parasuis. The resistance of quorum sensing system deficient G. parasuis to heat shock was obviously weaker than that of wild type strain. However, the regulatory mechanism of this phenotype remains unclear. To illustrate the regulatory mechanism by which the quorum sensing system provides resistance to heat shock, the transcriptomes of wild type (GPS2), ΔluxS, and luxS complemented (C-luxS) strains were analyzed. Four hundred forty-four differentially expressed genes were identified in quorum sensing system deficient G. parasuis, which participated in multiple regulatory pathways. Furthermore, we found that G. parasuis regulates the expression of rseA, rpoE, rseB, degS, clpP, and htrA genes to resist heat shock via the quorum sensing system. We further confirmed that rseA and rpoE genes exerted an opposite regulatory effect on heat shock resistance. In conclusion, the findings of this study provide a novel insight into how the quorum sensing system affects the transcriptome of G. parasuis and regulates its heat shock resistance property. |
| first_indexed | 2024-04-13T13:25:53Z |
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| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-13T13:25:53Z |
| publishDate | 2022-08-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-2cd80be91dae48968431a0f743cb03fe2022-12-22T02:45:08ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-08-011310.3389/fmicb.2022.968460968460Transcriptome analysis of heat resistance regulated by quorum sensing system in Glaesserella parasuisBingzhou Zhang0Bingzhou Zhang1Changsheng Jiang2Changsheng Jiang3Hua Cao4Hua Cao5Wei Zeng6Wei Zeng7Jingping Ren8Jingping Ren9Yaofang Hu10Yaofang Hu11Wentao Li12Wentao Li13Wentao Li14Qigai He15Qigai He16State Key Laboratory of Agricultural Microbiology, College of Animal Sciences and Veterinary Medicine, Huazhong Agricultural University, Wuhan, ChinaThe Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan, ChinaState Key Laboratory of Agricultural Microbiology, College of Animal Sciences and Veterinary Medicine, Huazhong Agricultural University, Wuhan, ChinaThe Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan, ChinaState Key Laboratory of Agricultural Microbiology, College of Animal Sciences and Veterinary Medicine, Huazhong Agricultural University, Wuhan, ChinaThe Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan, ChinaState Key Laboratory of Agricultural Microbiology, College of Animal Sciences and Veterinary Medicine, Huazhong Agricultural University, Wuhan, ChinaThe Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan, ChinaState Key Laboratory of Agricultural Microbiology, College of Animal Sciences and Veterinary Medicine, Huazhong Agricultural University, Wuhan, ChinaThe Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan, ChinaState Key Laboratory of Agricultural Microbiology, College of Animal Sciences and Veterinary Medicine, Huazhong Agricultural University, Wuhan, ChinaThe Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan, ChinaState Key Laboratory of Agricultural Microbiology, College of Animal Sciences and Veterinary Medicine, Huazhong Agricultural University, Wuhan, ChinaThe Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan, ChinaHubei Hongshan Laboratory, Wuhan, ChinaState Key Laboratory of Agricultural Microbiology, College of Animal Sciences and Veterinary Medicine, Huazhong Agricultural University, Wuhan, ChinaThe Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan, ChinaThe ability of bacteria to resist heat shock allows them to adapt to different environments. In addition, heat shock resistance is known for their virulence. Our previous study showed that the AI-2/luxS quorum sensing system affects the growth characteristics, biofilm formation, and virulence of Glaesserella parasuis. The resistance of quorum sensing system deficient G. parasuis to heat shock was obviously weaker than that of wild type strain. However, the regulatory mechanism of this phenotype remains unclear. To illustrate the regulatory mechanism by which the quorum sensing system provides resistance to heat shock, the transcriptomes of wild type (GPS2), ΔluxS, and luxS complemented (C-luxS) strains were analyzed. Four hundred forty-four differentially expressed genes were identified in quorum sensing system deficient G. parasuis, which participated in multiple regulatory pathways. Furthermore, we found that G. parasuis regulates the expression of rseA, rpoE, rseB, degS, clpP, and htrA genes to resist heat shock via the quorum sensing system. We further confirmed that rseA and rpoE genes exerted an opposite regulatory effect on heat shock resistance. In conclusion, the findings of this study provide a novel insight into how the quorum sensing system affects the transcriptome of G. parasuis and regulates its heat shock resistance property.https://www.frontiersin.org/articles/10.3389/fmicb.2022.968460/fullquorum sensingGlaesserella parasuistranscriptomeheat shock resistancemolecular mechanism |
| spellingShingle | Bingzhou Zhang Bingzhou Zhang Changsheng Jiang Changsheng Jiang Hua Cao Hua Cao Wei Zeng Wei Zeng Jingping Ren Jingping Ren Yaofang Hu Yaofang Hu Wentao Li Wentao Li Wentao Li Qigai He Qigai He Transcriptome analysis of heat resistance regulated by quorum sensing system in Glaesserella parasuis Frontiers in Microbiology quorum sensing Glaesserella parasuis transcriptome heat shock resistance molecular mechanism |
| title | Transcriptome analysis of heat resistance regulated by quorum sensing system in Glaesserella parasuis |
| title_full | Transcriptome analysis of heat resistance regulated by quorum sensing system in Glaesserella parasuis |
| title_fullStr | Transcriptome analysis of heat resistance regulated by quorum sensing system in Glaesserella parasuis |
| title_full_unstemmed | Transcriptome analysis of heat resistance regulated by quorum sensing system in Glaesserella parasuis |
| title_short | Transcriptome analysis of heat resistance regulated by quorum sensing system in Glaesserella parasuis |
| title_sort | transcriptome analysis of heat resistance regulated by quorum sensing system in glaesserella parasuis |
| topic | quorum sensing Glaesserella parasuis transcriptome heat shock resistance molecular mechanism |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.968460/full |
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