The Effect of Submicron Polystyrene on the Electrokinetic Potential of Cell Membranes of Red Blood Cells and Platelets
In recent years, many scientists have studied the effects of polymer micro- and nanostructures on living organisms. As it turns out, plastic can be a component of the blood of livestock, eaten by humans around the globe. Thus, it seems important to investigate possible changes in the physicochemical...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-03-01
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| Series: | Membranes |
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| Online Access: | https://www.mdpi.com/2077-0375/12/4/366 |
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| author | Marcin Zając Joanna Kotyńska Mateusz Worobiczuk Joanna Breczko Monika Naumowicz |
| author_facet | Marcin Zając Joanna Kotyńska Mateusz Worobiczuk Joanna Breczko Monika Naumowicz |
| author_sort | Marcin Zając |
| collection | DOAJ |
| description | In recent years, many scientists have studied the effects of polymer micro- and nanostructures on living organisms. As it turns out, plastic can be a component of the blood of livestock, eaten by humans around the globe. Thus, it seems important to investigate possible changes in the physicochemical parameters and morphology of the cell membranes of blood morphotic elements (red blood cells and platelets) under the influence of polymer particles. The article presents research in which cell membranes were exposed to plain polystyrene (PS) and amino-functionalized polystyrene (PS-NH<sub>2</sub>) of two different sizes. The polymers were characterized by infrared spectroscopy and dynamic light-scattering methods. To analyze possible changes caused by polymer exposure in the structure of the membranes, their zeta potentials were measured using the electrophoretic light-scattering technique. The concentration of the polymers, as well as the exposure time, were also taken into the consideration during the research. Based on the obtained results, we concluded that 100 and 200 nm PS, as well as 100 nm PS-NH<sub>2</sub>, internalize into the cells. On the contrary, 200 nm PS-NH<sub>2</sub> particles attach to cell membranes. Our study clearly shows that particle size and surface chemistry determine the interaction with biological membranes. |
| first_indexed | 2024-03-09T10:31:49Z |
| format | Article |
| id | doaj.art-2cd899ec499542d7985d61984f73d7fd |
| institution | Directory Open Access Journal |
| issn | 2077-0375 |
| language | English |
| last_indexed | 2024-03-09T10:31:49Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Membranes |
| spelling | doaj.art-2cd899ec499542d7985d61984f73d7fd2023-12-01T21:12:57ZengMDPI AGMembranes2077-03752022-03-0112436610.3390/membranes12040366The Effect of Submicron Polystyrene on the Electrokinetic Potential of Cell Membranes of Red Blood Cells and PlateletsMarcin Zając0Joanna Kotyńska1Mateusz Worobiczuk2Joanna Breczko3Monika Naumowicz4Doctoral School of Exact and Natural Sciences, University of Bialystok, K. Ciolkowskiego 1K, 15-245 Bialystok, PolandDepartment of Physical Chemistry, Faculty of Chemistry, University of Bialystok, K. Ciolkowskiego 1K, 15-245 Bialystok, PolandDepartment of Physical Chemistry, Faculty of Chemistry, University of Bialystok, K. Ciolkowskiego 1K, 15-245 Bialystok, PolandDepartment of Physical Chemistry, Faculty of Chemistry, University of Bialystok, K. Ciolkowskiego 1K, 15-245 Bialystok, PolandDepartment of Physical Chemistry, Faculty of Chemistry, University of Bialystok, K. Ciolkowskiego 1K, 15-245 Bialystok, PolandIn recent years, many scientists have studied the effects of polymer micro- and nanostructures on living organisms. As it turns out, plastic can be a component of the blood of livestock, eaten by humans around the globe. Thus, it seems important to investigate possible changes in the physicochemical parameters and morphology of the cell membranes of blood morphotic elements (red blood cells and platelets) under the influence of polymer particles. The article presents research in which cell membranes were exposed to plain polystyrene (PS) and amino-functionalized polystyrene (PS-NH<sub>2</sub>) of two different sizes. The polymers were characterized by infrared spectroscopy and dynamic light-scattering methods. To analyze possible changes caused by polymer exposure in the structure of the membranes, their zeta potentials were measured using the electrophoretic light-scattering technique. The concentration of the polymers, as well as the exposure time, were also taken into the consideration during the research. Based on the obtained results, we concluded that 100 and 200 nm PS, as well as 100 nm PS-NH<sub>2</sub>, internalize into the cells. On the contrary, 200 nm PS-NH<sub>2</sub> particles attach to cell membranes. Our study clearly shows that particle size and surface chemistry determine the interaction with biological membranes.https://www.mdpi.com/2077-0375/12/4/366polymerserythrocytesplateletselectrophoretic light scatteringdynamic light scatteringFTIR spectroscopy |
| spellingShingle | Marcin Zając Joanna Kotyńska Mateusz Worobiczuk Joanna Breczko Monika Naumowicz The Effect of Submicron Polystyrene on the Electrokinetic Potential of Cell Membranes of Red Blood Cells and Platelets Membranes polymers erythrocytes platelets electrophoretic light scattering dynamic light scattering FTIR spectroscopy |
| title | The Effect of Submicron Polystyrene on the Electrokinetic Potential of Cell Membranes of Red Blood Cells and Platelets |
| title_full | The Effect of Submicron Polystyrene on the Electrokinetic Potential of Cell Membranes of Red Blood Cells and Platelets |
| title_fullStr | The Effect of Submicron Polystyrene on the Electrokinetic Potential of Cell Membranes of Red Blood Cells and Platelets |
| title_full_unstemmed | The Effect of Submicron Polystyrene on the Electrokinetic Potential of Cell Membranes of Red Blood Cells and Platelets |
| title_short | The Effect of Submicron Polystyrene on the Electrokinetic Potential of Cell Membranes of Red Blood Cells and Platelets |
| title_sort | effect of submicron polystyrene on the electrokinetic potential of cell membranes of red blood cells and platelets |
| topic | polymers erythrocytes platelets electrophoretic light scattering dynamic light scattering FTIR spectroscopy |
| url | https://www.mdpi.com/2077-0375/12/4/366 |
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