In vitro regeneration of Centaurium erythraea Rafn from shoot tips and other seedling explants
Various explants from 30-day-old seedlings of Centaurium erythraea Rafn were evaluated for their morphogenetic capacity under in vitro culture conditions. Shoot formation from shoot tip explants was achieved mainly through adventitious bud differentiation. The highest number of shoots (up to 43.3 ±...
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| Format: | Article |
| Language: | English |
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Polish Botanical Society
2011-01-01
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| Series: | Acta Societatis Botanicorum Poloniae |
| Subjects: | |
| Online Access: | https://pbsociety.org.pl/journals/index.php/asbp/article/view/36 |
| _version_ | 1818517420586827776 |
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| author | Ewelina Piątczak Halina Wysokińska |
| author_facet | Ewelina Piątczak Halina Wysokińska |
| author_sort | Ewelina Piątczak |
| collection | DOAJ |
| description | Various explants from 30-day-old seedlings of Centaurium erythraea Rafn were evaluated for their morphogenetic capacity under in vitro culture conditions. Shoot formation from shoot tip explants was achieved mainly through adventitious bud differentiation. The highest number of shoots (up to 43.3 ± 2.2 from a single shoot tip) was obtained on Murashige and Skoog medium (MS) supplemented with indole-3-acetic acid (IAA) (0.57 μM) and 6-benzylaminopurine (BAP) (4.4 μM). Adventitious shoot regeneration was also achieved through organogenesis from calluses obtained from hypocotyls, cotyledons, roots and leaves on MS medium containing IAA (2.85 μM) and BAP (0.88 μM). Significant differences were noted between explant types in their effects on shoot regeneration. In the primary culture, the best response was obtained either from calluses derived from roots or leaves (44.4 ± 4.5 and 40.2 ± 6.0 shoots per callus, respectively). The number of subcultures of inoculated calluses affected both the multiplication rate (the number of shoots/explant) and shoot morphology (the frequency of shoot hyperhydricity). Shoots rooted with the frequency of 94-100% after culture on MS medium without growth regulators. Plantlets were successfully acclimatized (97%) under high relative humidity and then moved to the greenhouse. |
| first_indexed | 2024-12-11T00:56:03Z |
| format | Article |
| id | doaj.art-2cd8a780621c453d97c6ac724977aa3d |
| institution | Directory Open Access Journal |
| issn | 2083-9480 |
| language | English |
| last_indexed | 2024-12-11T00:56:03Z |
| publishDate | 2011-01-01 |
| publisher | Polish Botanical Society |
| record_format | Article |
| series | Acta Societatis Botanicorum Poloniae |
| spelling | doaj.art-2cd8a780621c453d97c6ac724977aa3d2022-12-22T01:26:29ZengPolish Botanical SocietyActa Societatis Botanicorum Poloniae2083-94802011-01-0172428328810.5586/asbp.2003.03636In vitro regeneration of Centaurium erythraea Rafn from shoot tips and other seedling explantsEwelina Piątczak0Halina Wysokińska1Medical University of ŁódźMedical University of ŁódźVarious explants from 30-day-old seedlings of Centaurium erythraea Rafn were evaluated for their morphogenetic capacity under in vitro culture conditions. Shoot formation from shoot tip explants was achieved mainly through adventitious bud differentiation. The highest number of shoots (up to 43.3 ± 2.2 from a single shoot tip) was obtained on Murashige and Skoog medium (MS) supplemented with indole-3-acetic acid (IAA) (0.57 μM) and 6-benzylaminopurine (BAP) (4.4 μM). Adventitious shoot regeneration was also achieved through organogenesis from calluses obtained from hypocotyls, cotyledons, roots and leaves on MS medium containing IAA (2.85 μM) and BAP (0.88 μM). Significant differences were noted between explant types in their effects on shoot regeneration. In the primary culture, the best response was obtained either from calluses derived from roots or leaves (44.4 ± 4.5 and 40.2 ± 6.0 shoots per callus, respectively). The number of subcultures of inoculated calluses affected both the multiplication rate (the number of shoots/explant) and shoot morphology (the frequency of shoot hyperhydricity). Shoots rooted with the frequency of 94-100% after culture on MS medium without growth regulators. Plantlets were successfully acclimatized (97%) under high relative humidity and then moved to the greenhouse.https://pbsociety.org.pl/journals/index.php/asbp/article/view/36Centaurium erythraea RafnGentianaceaemicropropagationorganogenesisadventitious shoots |
| spellingShingle | Ewelina Piątczak Halina Wysokińska In vitro regeneration of Centaurium erythraea Rafn from shoot tips and other seedling explants Acta Societatis Botanicorum Poloniae Centaurium erythraea Rafn Gentianaceae micropropagation organogenesis adventitious shoots |
| title | In vitro regeneration of Centaurium erythraea Rafn from shoot tips and other seedling explants |
| title_full | In vitro regeneration of Centaurium erythraea Rafn from shoot tips and other seedling explants |
| title_fullStr | In vitro regeneration of Centaurium erythraea Rafn from shoot tips and other seedling explants |
| title_full_unstemmed | In vitro regeneration of Centaurium erythraea Rafn from shoot tips and other seedling explants |
| title_short | In vitro regeneration of Centaurium erythraea Rafn from shoot tips and other seedling explants |
| title_sort | in vitro regeneration of centaurium erythraea rafn from shoot tips and other seedling explants |
| topic | Centaurium erythraea Rafn Gentianaceae micropropagation organogenesis adventitious shoots |
| url | https://pbsociety.org.pl/journals/index.php/asbp/article/view/36 |
| work_keys_str_mv | AT ewelinapiatczak invitroregenerationofcentauriumerythraearafnfromshoottipsandotherseedlingexplants AT halinawysokinska invitroregenerationofcentauriumerythraearafnfromshoottipsandotherseedlingexplants |