Primary breast cancer biomarkers based on glycosylation and extracellular vesicles detected from human serum
Abstract Background Breast cancer is a very common cancer that can be severe if not discovered early. The current tools to detect breast cancer need improvement. Cancer has a universal tendency to affect glycosylation. The glycosylation of circulating extracellular vesicle‐associated glycoproteins,...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2022-08-01
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| Series: | Cancer Reports |
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| Online Access: | https://doi.org/10.1002/cnr2.1540 |
| _version_ | 1818172478903549952 |
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| author | Joonas Terävä Alejandra Verhassel Orsola Botti Md. Khirul Islam Janne Leivo Saara Wittfooth Pirkko Härkönen Kim Pettersson Kamlesh Gidwani |
| author_facet | Joonas Terävä Alejandra Verhassel Orsola Botti Md. Khirul Islam Janne Leivo Saara Wittfooth Pirkko Härkönen Kim Pettersson Kamlesh Gidwani |
| author_sort | Joonas Terävä |
| collection | DOAJ |
| description | Abstract Background Breast cancer is a very common cancer that can be severe if not discovered early. The current tools to detect breast cancer need improvement. Cancer has a universal tendency to affect glycosylation. The glycosylation of circulating extracellular vesicle‐associated glycoproteins, and mucins may offer targets for detection methods and have been only explored in a limited capacity. Aim Our aim was to develop an approach to detect the aberrant glycosylation of mucins and extracellular vesicle‐associated glycoproteins from human sera using fluorescent nanoparticles, and preliminarily evaluate this approach for the differential diagnosis of breast cancer. Methods and results The assay involved immobilizing glycosylated antigens using monoclonal antibodies and then probing their glycosylation by using lectins and glycan‐specific antibodies coated on Eu+3‐doped nanoparticles. Detection of mucin 1 and mucin 16 glycosylation with wheat germ agglutinin, and detection of the extracellular vesicle‐associated CD63 were found to have better diagnostic ability for localized breast cancer than the conventional assays for mucin 1 and mucin 16 based tumor markers when the receiver operating characteristics were compared. Conclusions These results indicate that successful differential diagnosis of primary breast cancer may be aided by detecting cancer‐associated glycosylation of mucin 1 and mucin 16, and total concentration of CD63, in human serum. |
| first_indexed | 2024-12-11T19:13:15Z |
| format | Article |
| id | doaj.art-2cd8c0c80d984699aa505fa5c772e6eb |
| institution | Directory Open Access Journal |
| issn | 2573-8348 |
| language | English |
| last_indexed | 2024-12-11T19:13:15Z |
| publishDate | 2022-08-01 |
| publisher | Wiley |
| record_format | Article |
| series | Cancer Reports |
| spelling | doaj.art-2cd8c0c80d984699aa505fa5c772e6eb2022-12-22T00:53:42ZengWileyCancer Reports2573-83482022-08-0158n/an/a10.1002/cnr2.1540Primary breast cancer biomarkers based on glycosylation and extracellular vesicles detected from human serumJoonas Terävä0Alejandra Verhassel1Orsola Botti2Md. Khirul Islam3Janne Leivo4Saara Wittfooth5Pirkko Härkönen6Kim Pettersson7Kamlesh Gidwani8Department of Biochemistry University of Turku Turku FinlandInstitute of Biomedicine and FICAN West Cancer Research Laboratory University of Turku Turku FinlandDepartment of Biochemistry University of Turku Turku FinlandDepartment of Biochemistry University of Turku Turku FinlandDepartment of Biochemistry University of Turku Turku FinlandDepartment of Biochemistry University of Turku Turku FinlandInstitute of Biomedicine and FICAN West Cancer Research Laboratory University of Turku Turku FinlandDepartment of Biochemistry University of Turku Turku FinlandDepartment of Biochemistry University of Turku Turku FinlandAbstract Background Breast cancer is a very common cancer that can be severe if not discovered early. The current tools to detect breast cancer need improvement. Cancer has a universal tendency to affect glycosylation. The glycosylation of circulating extracellular vesicle‐associated glycoproteins, and mucins may offer targets for detection methods and have been only explored in a limited capacity. Aim Our aim was to develop an approach to detect the aberrant glycosylation of mucins and extracellular vesicle‐associated glycoproteins from human sera using fluorescent nanoparticles, and preliminarily evaluate this approach for the differential diagnosis of breast cancer. Methods and results The assay involved immobilizing glycosylated antigens using monoclonal antibodies and then probing their glycosylation by using lectins and glycan‐specific antibodies coated on Eu+3‐doped nanoparticles. Detection of mucin 1 and mucin 16 glycosylation with wheat germ agglutinin, and detection of the extracellular vesicle‐associated CD63 were found to have better diagnostic ability for localized breast cancer than the conventional assays for mucin 1 and mucin 16 based tumor markers when the receiver operating characteristics were compared. Conclusions These results indicate that successful differential diagnosis of primary breast cancer may be aided by detecting cancer‐associated glycosylation of mucin 1 and mucin 16, and total concentration of CD63, in human serum.https://doi.org/10.1002/cnr2.1540breast neoplasmsglycosylationin vitro diagnosticsmucinstetraspannin‐30 |
| spellingShingle | Joonas Terävä Alejandra Verhassel Orsola Botti Md. Khirul Islam Janne Leivo Saara Wittfooth Pirkko Härkönen Kim Pettersson Kamlesh Gidwani Primary breast cancer biomarkers based on glycosylation and extracellular vesicles detected from human serum Cancer Reports breast neoplasms glycosylation in vitro diagnostics mucins tetraspannin‐30 |
| title | Primary breast cancer biomarkers based on glycosylation and extracellular vesicles detected from human serum |
| title_full | Primary breast cancer biomarkers based on glycosylation and extracellular vesicles detected from human serum |
| title_fullStr | Primary breast cancer biomarkers based on glycosylation and extracellular vesicles detected from human serum |
| title_full_unstemmed | Primary breast cancer biomarkers based on glycosylation and extracellular vesicles detected from human serum |
| title_short | Primary breast cancer biomarkers based on glycosylation and extracellular vesicles detected from human serum |
| title_sort | primary breast cancer biomarkers based on glycosylation and extracellular vesicles detected from human serum |
| topic | breast neoplasms glycosylation in vitro diagnostics mucins tetraspannin‐30 |
| url | https://doi.org/10.1002/cnr2.1540 |
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