Experimental chronic hepatitis B infection of neonatal tree shrews (<it>Tupaia belangeri chinensis</it>): A model to study molecular causes for susceptibility and disease progression to chronic hepatitis in humans
<p>Abstract</p> <p>Background</p> <p>Hepatitis B virus (HBV) infection continues to be an escalating global health problem. Feasible and effective animal models for HBV infection are the prerequisite for developing novel therapies for this disease. The tree shrew (<i...
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| Format: | Article |
| Language: | English |
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BMC
2012-08-01
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| Series: | Virology Journal |
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| Online Access: | http://www.virologyj.com/content/9/1/170 |
| _version_ | 1818363080781856768 |
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| author | Wang Qi Schwarzenberger Paul Yang Fang Zhang Jingjing Su Jianjia Yang Chun Cao Ji Ou Chao Liang Liang Shi Junlin Yang Fang Wang Duoping Wang Jia Wang Xiaojuan Ruan Ping Li Yuan |
| author_facet | Wang Qi Schwarzenberger Paul Yang Fang Zhang Jingjing Su Jianjia Yang Chun Cao Ji Ou Chao Liang Liang Shi Junlin Yang Fang Wang Duoping Wang Jia Wang Xiaojuan Ruan Ping Li Yuan |
| author_sort | Wang Qi |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Hepatitis B virus (HBV) infection continues to be an escalating global health problem. Feasible and effective animal models for HBV infection are the prerequisite for developing novel therapies for this disease. The tree shrew (<it>Tupaia</it>) is a small animal species evolutionary closely related to humans, and thus is permissive to certain human viral pathogens. Whether tree shrews could be chronically infected with HBV in vivo has been controversial for decades. Most published research has been reported on adult tree shrews, and only small numbers of HBV infected newborn tree shrews had been observed over short time periods. We investigated susceptibility of newborn tree shrews to experimental HBV infection as well as viral clearance over a protracted time period.</p> <p>Results</p> <p>Forty-six newborn tree shrews were inoculated with the sera from HBV-infected patients or tree shrews. Serum and liver samples of the inoculated animals were periodically collected and analyzed using fluorescence quantitative polymerase chain reaction, enzyme-linked immunosorbent assay, Southern blot, and immunohistochemistry. Six tree shrews were confirmed and four were suspected as chronically HBV-infected for more than 48 (up to 228) weeks after inoculation, including three that had been inoculated with serum from a confirmed HBV-infected tree shrew.</p> <p>Conclusions</p> <p>Outbred neonatal tree shrews can be long-term chronically infected with HBV at a frequency comparable to humans. The model resembles human disease where also a smaller proportion of infected individuals develop chronic HBV related disease. This model might enable genetic and immunologic investigations which would allow determination of underlying molecular causes favoring susceptibility for chronic HBV infection and disease establishment vs. viral clearance.</p> |
| first_indexed | 2024-12-13T21:42:48Z |
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| institution | Directory Open Access Journal |
| issn | 1743-422X |
| language | English |
| last_indexed | 2024-12-13T21:42:48Z |
| publishDate | 2012-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Virology Journal |
| spelling | doaj.art-2cd8d3fd0b44443d9ac30fa90f7da1d62022-12-21T23:30:29ZengBMCVirology Journal1743-422X2012-08-019117010.1186/1743-422X-9-170Experimental chronic hepatitis B infection of neonatal tree shrews (<it>Tupaia belangeri chinensis</it>): A model to study molecular causes for susceptibility and disease progression to chronic hepatitis in humansWang QiSchwarzenberger PaulYang FangZhang JingjingSu JianjiaYang ChunCao JiOu ChaoLiang LiangShi JunlinYang FangWang DuopingWang JiaWang XiaojuanRuan PingLi Yuan<p>Abstract</p> <p>Background</p> <p>Hepatitis B virus (HBV) infection continues to be an escalating global health problem. Feasible and effective animal models for HBV infection are the prerequisite for developing novel therapies for this disease. The tree shrew (<it>Tupaia</it>) is a small animal species evolutionary closely related to humans, and thus is permissive to certain human viral pathogens. Whether tree shrews could be chronically infected with HBV in vivo has been controversial for decades. Most published research has been reported on adult tree shrews, and only small numbers of HBV infected newborn tree shrews had been observed over short time periods. We investigated susceptibility of newborn tree shrews to experimental HBV infection as well as viral clearance over a protracted time period.</p> <p>Results</p> <p>Forty-six newborn tree shrews were inoculated with the sera from HBV-infected patients or tree shrews. Serum and liver samples of the inoculated animals were periodically collected and analyzed using fluorescence quantitative polymerase chain reaction, enzyme-linked immunosorbent assay, Southern blot, and immunohistochemistry. Six tree shrews were confirmed and four were suspected as chronically HBV-infected for more than 48 (up to 228) weeks after inoculation, including three that had been inoculated with serum from a confirmed HBV-infected tree shrew.</p> <p>Conclusions</p> <p>Outbred neonatal tree shrews can be long-term chronically infected with HBV at a frequency comparable to humans. The model resembles human disease where also a smaller proportion of infected individuals develop chronic HBV related disease. This model might enable genetic and immunologic investigations which would allow determination of underlying molecular causes favoring susceptibility for chronic HBV infection and disease establishment vs. viral clearance.</p>http://www.virologyj.com/content/9/1/170Tree shrew (<it>Tupaia</it>)Hepatitis B virusChronic infection |
| spellingShingle | Wang Qi Schwarzenberger Paul Yang Fang Zhang Jingjing Su Jianjia Yang Chun Cao Ji Ou Chao Liang Liang Shi Junlin Yang Fang Wang Duoping Wang Jia Wang Xiaojuan Ruan Ping Li Yuan Experimental chronic hepatitis B infection of neonatal tree shrews (<it>Tupaia belangeri chinensis</it>): A model to study molecular causes for susceptibility and disease progression to chronic hepatitis in humans Virology Journal Tree shrew (<it>Tupaia</it>) Hepatitis B virus Chronic infection |
| title | Experimental chronic hepatitis B infection of neonatal tree shrews (<it>Tupaia belangeri chinensis</it>): A model to study molecular causes for susceptibility and disease progression to chronic hepatitis in humans |
| title_full | Experimental chronic hepatitis B infection of neonatal tree shrews (<it>Tupaia belangeri chinensis</it>): A model to study molecular causes for susceptibility and disease progression to chronic hepatitis in humans |
| title_fullStr | Experimental chronic hepatitis B infection of neonatal tree shrews (<it>Tupaia belangeri chinensis</it>): A model to study molecular causes for susceptibility and disease progression to chronic hepatitis in humans |
| title_full_unstemmed | Experimental chronic hepatitis B infection of neonatal tree shrews (<it>Tupaia belangeri chinensis</it>): A model to study molecular causes for susceptibility and disease progression to chronic hepatitis in humans |
| title_short | Experimental chronic hepatitis B infection of neonatal tree shrews (<it>Tupaia belangeri chinensis</it>): A model to study molecular causes for susceptibility and disease progression to chronic hepatitis in humans |
| title_sort | experimental chronic hepatitis b infection of neonatal tree shrews it tupaia belangeri chinensis it a model to study molecular causes for susceptibility and disease progression to chronic hepatitis in humans |
| topic | Tree shrew (<it>Tupaia</it>) Hepatitis B virus Chronic infection |
| url | http://www.virologyj.com/content/9/1/170 |
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