Long-Term Viability of Isolated Bovine Adrenal Medullary Chromaffin Cells following Intrastriatal Transplantation
Adrenal medullary grafts generally exhibit poor viability when grafted into the striatum. Previous work in our laboratory demonstrated that chromaffin cells can survive well for up to 2 mo following grafting into the intact rat striatum after cells are isolated from the nonchromaffin supporting cell...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
1995-01-01
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| Series: | Cell Transplantation |
| Online Access: | https://doi.org/10.1177/096368979500400109 |
| _version_ | 1818912903210729472 |
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| author | Sherry B. Schueler Jacqueline Sagen George D. Pappas Jeffrey H. Kordower |
| author_facet | Sherry B. Schueler Jacqueline Sagen George D. Pappas Jeffrey H. Kordower |
| author_sort | Sherry B. Schueler |
| collection | DOAJ |
| description | Adrenal medullary grafts generally exhibit poor viability when grafted into the striatum. Previous work in our laboratory demonstrated that chromaffin cells can survive well for up to 2 mo following grafting into the intact rat striatum after cells are isolated from the nonchromaffin supporting cells (fibroblasts and endothelial cells) of the adrenal medulla. The aim of the present study was to assess the long-term viability of isolated bovine chromaffin cells following grafting into the intact rat striatum. The viability of grafted bovine adrenal medullary chromaffin cells was compared in rats receiving either (a) perfused adrenal medulla; (b) isolated chromaffin cells; or (c) isolated chromaffin cells that were subsequently recombined with their nonchromaffin supporting cells. One year postimplantation, all graft types which included fibroblasts and endothelial cells were infiltrated with macrophages and demonstrated an abundance of cellular debris. No viable chromaffin cells were observed. In contrast, healthy tyrosine hydroxylase (TH) and dopamine beta hydroxylase (DβH) immunoreactive chromaffin cells survived for 1 yr posttransplantation when grafted in isolation from the nonchromaffin constituents of the adrenal medulla. Good xenograft survival was achieved in this group despite the fact that these rats were only immunosuppressed for 1 mo postimplantation. Grafted cells demonstrated morphological characteristics of chromaffin cells in situ and these implants were not accompanied by macrophage infiltration. These data demonstrate that long-term survival of chromaffin cells can be achieved following intrastriatal implantation and the viability of grafted chromaffin cells is dependent upon the removal of the nonchromaffin supporting cells. |
| first_indexed | 2024-12-19T23:21:59Z |
| format | Article |
| id | doaj.art-2cda209cfda94d0e9220abadef553a60 |
| institution | Directory Open Access Journal |
| issn | 0963-6897 1555-3892 |
| language | English |
| last_indexed | 2024-12-19T23:21:59Z |
| publishDate | 1995-01-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Cell Transplantation |
| spelling | doaj.art-2cda209cfda94d0e9220abadef553a602022-12-21T20:01:57ZengSAGE PublishingCell Transplantation0963-68971555-38921995-01-01410.1177/096368979500400109Long-Term Viability of Isolated Bovine Adrenal Medullary Chromaffin Cells following Intrastriatal TransplantationSherry B. Schueler0Jacqueline Sagen1George D. Pappas2Jeffrey H. Kordower3Department of Anatomy and Cell Biology, University of Illinois School of Medicine, Chicago, IL 60612 andDepartment of Anatomy and Cell Biology, University of Illinois School of Medicine, Chicago, IL 60612 andDepartment of Anatomy and Cell Biology, University of Illinois School of Medicine, Chicago, IL 60612 andDepartment of Neurological Sciences, Rush Presbyterian Medical Center, 2242 West Harrision Street, Chicago, IL 60612Adrenal medullary grafts generally exhibit poor viability when grafted into the striatum. Previous work in our laboratory demonstrated that chromaffin cells can survive well for up to 2 mo following grafting into the intact rat striatum after cells are isolated from the nonchromaffin supporting cells (fibroblasts and endothelial cells) of the adrenal medulla. The aim of the present study was to assess the long-term viability of isolated bovine chromaffin cells following grafting into the intact rat striatum. The viability of grafted bovine adrenal medullary chromaffin cells was compared in rats receiving either (a) perfused adrenal medulla; (b) isolated chromaffin cells; or (c) isolated chromaffin cells that were subsequently recombined with their nonchromaffin supporting cells. One year postimplantation, all graft types which included fibroblasts and endothelial cells were infiltrated with macrophages and demonstrated an abundance of cellular debris. No viable chromaffin cells were observed. In contrast, healthy tyrosine hydroxylase (TH) and dopamine beta hydroxylase (DβH) immunoreactive chromaffin cells survived for 1 yr posttransplantation when grafted in isolation from the nonchromaffin constituents of the adrenal medulla. Good xenograft survival was achieved in this group despite the fact that these rats were only immunosuppressed for 1 mo postimplantation. Grafted cells demonstrated morphological characteristics of chromaffin cells in situ and these implants were not accompanied by macrophage infiltration. These data demonstrate that long-term survival of chromaffin cells can be achieved following intrastriatal implantation and the viability of grafted chromaffin cells is dependent upon the removal of the nonchromaffin supporting cells.https://doi.org/10.1177/096368979500400109 |
| spellingShingle | Sherry B. Schueler Jacqueline Sagen George D. Pappas Jeffrey H. Kordower Long-Term Viability of Isolated Bovine Adrenal Medullary Chromaffin Cells following Intrastriatal Transplantation Cell Transplantation |
| title | Long-Term Viability of Isolated Bovine Adrenal Medullary Chromaffin Cells following Intrastriatal Transplantation |
| title_full | Long-Term Viability of Isolated Bovine Adrenal Medullary Chromaffin Cells following Intrastriatal Transplantation |
| title_fullStr | Long-Term Viability of Isolated Bovine Adrenal Medullary Chromaffin Cells following Intrastriatal Transplantation |
| title_full_unstemmed | Long-Term Viability of Isolated Bovine Adrenal Medullary Chromaffin Cells following Intrastriatal Transplantation |
| title_short | Long-Term Viability of Isolated Bovine Adrenal Medullary Chromaffin Cells following Intrastriatal Transplantation |
| title_sort | long term viability of isolated bovine adrenal medullary chromaffin cells following intrastriatal transplantation |
| url | https://doi.org/10.1177/096368979500400109 |
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