Transcriptome Analysis of Biochemistry Responses to Low-Temperature Stress in the Flower Organs of Five Pear Varieties
Using floral organs of five pear cultivars as materials, this study determined and compared physiological indices such as relative conductivity, superoxide dismutase (SOD), and malondialdehyde (MDA) of each cultivar’s floral organs under different low-temperature stress treatments, and evaluated the...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-02-01
|
| Series: | Forests |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1999-4907/14/3/490 |
| _version_ | 1797611653123014656 |
|---|---|
| author | Shun Lin Yongtan Li Jingxian Zhao Weizhen Guo Min Jiang Xinman Li Weiping Liu Jun Zhang Minsheng Yang |
| author_facet | Shun Lin Yongtan Li Jingxian Zhao Weizhen Guo Min Jiang Xinman Li Weiping Liu Jun Zhang Minsheng Yang |
| author_sort | Shun Lin |
| collection | DOAJ |
| description | Using floral organs of five pear cultivars as materials, this study determined and compared physiological indices such as relative conductivity, superoxide dismutase (SOD), and malondialdehyde (MDA) of each cultivar’s floral organs under different low-temperature stress treatments, and evaluated the cold resistance of the five pear cultivars. Transcriptome sequencing analysis was performed on the floral organs of a new early-ripening pear cultivar called “Jinguang”, and 259 differentially expressed genes (DEGs) were identified, which were mainly enriched in pathways related to circadian rhythm and flavonoid biosynthesis. Weighted gene co-expression network analysis (WGCNA) showed that specific gene modules were significantly associated with MDA and soluble protein. Key enzymes such as NPC1(non-specific PLC, NPC), transcription factor MYB102, BBX19, and LHY (Late elongated hypocotyl) genes were located at the core of the constructed network, and may have important potential roles under low-temperature stress. |
| first_indexed | 2024-03-11T06:31:45Z |
| format | Article |
| id | doaj.art-2cda2a4553044bac9c5247c4249aabd9 |
| institution | Directory Open Access Journal |
| issn | 1999-4907 |
| language | English |
| last_indexed | 2024-03-11T06:31:45Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Forests |
| spelling | doaj.art-2cda2a4553044bac9c5247c4249aabd92023-11-17T11:09:20ZengMDPI AGForests1999-49072023-02-0114349010.3390/f14030490Transcriptome Analysis of Biochemistry Responses to Low-Temperature Stress in the Flower Organs of Five Pear VarietiesShun Lin0Yongtan Li1Jingxian Zhao2Weizhen Guo3Min Jiang4Xinman Li5Weiping Liu6Jun Zhang7Minsheng Yang8Forest Department, Forestry College, Hebei Agricultural University, Baoding 071000, ChinaForest Department, Forestry College, Hebei Agricultural University, Baoding 071000, ChinaHebei Academy of Forestry and Grassland Sciences, Shijiazhuang 050000, ChinaHebei Academy of Forestry and Grassland Sciences, Shijiazhuang 050000, ChinaForest Department, Forestry College, Hebei Agricultural University, Baoding 071000, ChinaForest Department, Forestry College, Hebei Agricultural University, Baoding 071000, ChinaForest Department, Forestry College, Hebei Agricultural University, Baoding 071000, ChinaForest Department, Forestry College, Hebei Agricultural University, Baoding 071000, ChinaForest Department, Forestry College, Hebei Agricultural University, Baoding 071000, ChinaUsing floral organs of five pear cultivars as materials, this study determined and compared physiological indices such as relative conductivity, superoxide dismutase (SOD), and malondialdehyde (MDA) of each cultivar’s floral organs under different low-temperature stress treatments, and evaluated the cold resistance of the five pear cultivars. Transcriptome sequencing analysis was performed on the floral organs of a new early-ripening pear cultivar called “Jinguang”, and 259 differentially expressed genes (DEGs) were identified, which were mainly enriched in pathways related to circadian rhythm and flavonoid biosynthesis. Weighted gene co-expression network analysis (WGCNA) showed that specific gene modules were significantly associated with MDA and soluble protein. Key enzymes such as NPC1(non-specific PLC, NPC), transcription factor MYB102, BBX19, and LHY (Late elongated hypocotyl) genes were located at the core of the constructed network, and may have important potential roles under low-temperature stress.https://www.mdpi.com/1999-4907/14/3/490pearcold stressbiochemistry traitstranscriptomeWGCNA |
| spellingShingle | Shun Lin Yongtan Li Jingxian Zhao Weizhen Guo Min Jiang Xinman Li Weiping Liu Jun Zhang Minsheng Yang Transcriptome Analysis of Biochemistry Responses to Low-Temperature Stress in the Flower Organs of Five Pear Varieties Forests pear cold stress biochemistry traits transcriptome WGCNA |
| title | Transcriptome Analysis of Biochemistry Responses to Low-Temperature Stress in the Flower Organs of Five Pear Varieties |
| title_full | Transcriptome Analysis of Biochemistry Responses to Low-Temperature Stress in the Flower Organs of Five Pear Varieties |
| title_fullStr | Transcriptome Analysis of Biochemistry Responses to Low-Temperature Stress in the Flower Organs of Five Pear Varieties |
| title_full_unstemmed | Transcriptome Analysis of Biochemistry Responses to Low-Temperature Stress in the Flower Organs of Five Pear Varieties |
| title_short | Transcriptome Analysis of Biochemistry Responses to Low-Temperature Stress in the Flower Organs of Five Pear Varieties |
| title_sort | transcriptome analysis of biochemistry responses to low temperature stress in the flower organs of five pear varieties |
| topic | pear cold stress biochemistry traits transcriptome WGCNA |
| url | https://www.mdpi.com/1999-4907/14/3/490 |
| work_keys_str_mv | AT shunlin transcriptomeanalysisofbiochemistryresponsestolowtemperaturestressintheflowerorgansoffivepearvarieties AT yongtanli transcriptomeanalysisofbiochemistryresponsestolowtemperaturestressintheflowerorgansoffivepearvarieties AT jingxianzhao transcriptomeanalysisofbiochemistryresponsestolowtemperaturestressintheflowerorgansoffivepearvarieties AT weizhenguo transcriptomeanalysisofbiochemistryresponsestolowtemperaturestressintheflowerorgansoffivepearvarieties AT minjiang transcriptomeanalysisofbiochemistryresponsestolowtemperaturestressintheflowerorgansoffivepearvarieties AT xinmanli transcriptomeanalysisofbiochemistryresponsestolowtemperaturestressintheflowerorgansoffivepearvarieties AT weipingliu transcriptomeanalysisofbiochemistryresponsestolowtemperaturestressintheflowerorgansoffivepearvarieties AT junzhang transcriptomeanalysisofbiochemistryresponsestolowtemperaturestressintheflowerorgansoffivepearvarieties AT minshengyang transcriptomeanalysisofbiochemistryresponsestolowtemperaturestressintheflowerorgansoffivepearvarieties |