Cdc13 (cyclin B) is degraded by autophagy under sulfur depletion in fission yeast
Cyclins are degraded by the anaphase-promoting complex/cyclosome (APC/C)-mediated proteasome in normal mitosis. We showed that Cdc13 (cyclin B) is also degraded by macroautophagy/autophagy in sulfur-deficient fission yeast. Sulfur depletion causes G2 cell cycle arrest and reduces cell size; however,...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2022-12-01
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| Series: | Autophagy Reports |
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| Online Access: | http://dx.doi.org/10.1080/27694127.2022.2047442 |
| _version_ | 1797685692049915904 |
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| author | Hokuto Ohtsuka Yoshiko Hatta Kana Hayashi Takafumi Shimasaki Yoko Otsubo Yurika Ito Yu Tsutsui Nobutake Hattori Akira Yamashita Hiroshi Murakami Hirofumi Aiba |
| author_facet | Hokuto Ohtsuka Yoshiko Hatta Kana Hayashi Takafumi Shimasaki Yoko Otsubo Yurika Ito Yu Tsutsui Nobutake Hattori Akira Yamashita Hiroshi Murakami Hirofumi Aiba |
| author_sort | Hokuto Ohtsuka |
| collection | DOAJ |
| description | Cyclins are degraded by the anaphase-promoting complex/cyclosome (APC/C)-mediated proteasome in normal mitosis. We showed that Cdc13 (cyclin B) is also degraded by macroautophagy/autophagy in sulfur-deficient fission yeast. Sulfur depletion causes G2 cell cycle arrest and reduces cell size; however, the associated mechanisms are unknown. We found that autophagy is required for the degradation of Cdc13, which is associated with cell cycle arrest and reduced cell size, by examining cell morphology under sulfur depletion. The analysis of the Cdc13-GFP fusion protein supported the conclusion that Cdc13 is degraded by autophagy. Moreover, we showed that sulfur depletion results in the inactivation of target of rapamycin complex 1 (TORC1) activity via Ecl1-family proteins. Our data indicate that the cyclin is degraded by two different systems: APC/C-mediated proteasome and autophagy. The latter is induced under nutrient-depleted situations. This switch in degradation systems will contribute to appropriate cell cycle arrest when resources are depleted. Abbreviations: APC, anaphase-promoting complex; CDK, cyclin-dependent kinase; DB, destruction box; EMM, Edinburgh minimal medium; GFP, green fluorescent protein; PCR, polymerase chain reaction; TOR, target of rapamycin; UPS, ubiquitin-proteasome system |
| first_indexed | 2024-03-12T00:55:00Z |
| format | Article |
| id | doaj.art-2cddbfddda1940d9ad3021797f5970cf |
| institution | Directory Open Access Journal |
| issn | 2769-4127 |
| language | English |
| last_indexed | 2024-03-12T00:55:00Z |
| publishDate | 2022-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Autophagy Reports |
| spelling | doaj.art-2cddbfddda1940d9ad3021797f5970cf2023-09-14T13:24:40ZengTaylor & Francis GroupAutophagy Reports2769-41272022-12-0111516410.1080/27694127.2022.20474422047442Cdc13 (cyclin B) is degraded by autophagy under sulfur depletion in fission yeastHokuto Ohtsuka0Yoshiko Hatta1Kana Hayashi2Takafumi Shimasaki3Yoko Otsubo4Yurika Ito5Yu Tsutsui6Nobutake Hattori7Akira Yamashita8Hiroshi Murakami9Hirofumi Aiba10Laboratory of Molecular Microbiology, Graduate School of Pharmaceutical Sciences, Nagoya University; Nagoya, AichiLaboratory of Molecular Microbiology, Graduate School of Pharmaceutical Sciences, Nagoya University; Nagoya, AichiLaboratory of Molecular Microbiology, Graduate School of Pharmaceutical Sciences, Nagoya University; Nagoya, AichiLaboratory of Molecular Microbiology, Graduate School of Pharmaceutical Sciences, Nagoya University; Nagoya, AichiLaboratory of Cell Responses, National Institute for Basic Biology; Okazaki, AichiLaboratory of Molecular Microbiology, Graduate School of Pharmaceutical Sciences, Nagoya University; Nagoya, AichiLaboratory of Molecular Microbiology, Graduate School of Pharmaceutical Sciences, Nagoya University; Nagoya, AichiLaboratory of Molecular Microbiology, Graduate School of Pharmaceutical Sciences, Nagoya University; Nagoya, AichiLaboratory of Cell Responses, National Institute for Basic Biology; Okazaki, AichiChuo University; TokyoLaboratory of Molecular Microbiology, Graduate School of Pharmaceutical Sciences, Nagoya University; Nagoya, AichiCyclins are degraded by the anaphase-promoting complex/cyclosome (APC/C)-mediated proteasome in normal mitosis. We showed that Cdc13 (cyclin B) is also degraded by macroautophagy/autophagy in sulfur-deficient fission yeast. Sulfur depletion causes G2 cell cycle arrest and reduces cell size; however, the associated mechanisms are unknown. We found that autophagy is required for the degradation of Cdc13, which is associated with cell cycle arrest and reduced cell size, by examining cell morphology under sulfur depletion. The analysis of the Cdc13-GFP fusion protein supported the conclusion that Cdc13 is degraded by autophagy. Moreover, we showed that sulfur depletion results in the inactivation of target of rapamycin complex 1 (TORC1) activity via Ecl1-family proteins. Our data indicate that the cyclin is degraded by two different systems: APC/C-mediated proteasome and autophagy. The latter is induced under nutrient-depleted situations. This switch in degradation systems will contribute to appropriate cell cycle arrest when resources are depleted. Abbreviations: APC, anaphase-promoting complex; CDK, cyclin-dependent kinase; DB, destruction box; EMM, Edinburgh minimal medium; GFP, green fluorescent protein; PCR, polymerase chain reaction; TOR, target of rapamycin; UPS, ubiquitin-proteasome systemhttp://dx.doi.org/10.1080/27694127.2022.2047442autophagycyclinecl1-family genefission yeasttorc1 |
| spellingShingle | Hokuto Ohtsuka Yoshiko Hatta Kana Hayashi Takafumi Shimasaki Yoko Otsubo Yurika Ito Yu Tsutsui Nobutake Hattori Akira Yamashita Hiroshi Murakami Hirofumi Aiba Cdc13 (cyclin B) is degraded by autophagy under sulfur depletion in fission yeast Autophagy Reports autophagy cyclin ecl1-family gene fission yeast torc1 |
| title | Cdc13 (cyclin B) is degraded by autophagy under sulfur depletion in fission yeast |
| title_full | Cdc13 (cyclin B) is degraded by autophagy under sulfur depletion in fission yeast |
| title_fullStr | Cdc13 (cyclin B) is degraded by autophagy under sulfur depletion in fission yeast |
| title_full_unstemmed | Cdc13 (cyclin B) is degraded by autophagy under sulfur depletion in fission yeast |
| title_short | Cdc13 (cyclin B) is degraded by autophagy under sulfur depletion in fission yeast |
| title_sort | cdc13 cyclin b is degraded by autophagy under sulfur depletion in fission yeast |
| topic | autophagy cyclin ecl1-family gene fission yeast torc1 |
| url | http://dx.doi.org/10.1080/27694127.2022.2047442 |
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