Variants in the interleukin 8 gene and the response to inhaled bronchodilators in cystic fibrosis
Objective: Interleukin 8 protein promotes inflammatory responses, even in airways. The presence of interleukin 8 gene variants causes altered inflammatory responses and possibly varied responses to inhaled bronchodilators. Thus, this study analyzed the interleukin 8 variants (rs4073, rs2227306, and...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | Portuguese |
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Brazilian Society of Pediatrics
2017-11-01
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| Series: | Jornal de Pediatria (Versão em Português) |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2255553617300794 |
| _version_ | 1811248728708743168 |
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| author | Larissa Lazzarini Furlan José Dirceu Ribeiro Carmen Sílvia Bertuzzo João Batista Salomão Junior Dorotéia Rossi Silva Souza Fernando Augusto Lima Marson |
| author_facet | Larissa Lazzarini Furlan José Dirceu Ribeiro Carmen Sílvia Bertuzzo João Batista Salomão Junior Dorotéia Rossi Silva Souza Fernando Augusto Lima Marson |
| author_sort | Larissa Lazzarini Furlan |
| collection | DOAJ |
| description | Objective: Interleukin 8 protein promotes inflammatory responses, even in airways. The presence of interleukin 8 gene variants causes altered inflammatory responses and possibly varied responses to inhaled bronchodilators. Thus, this study analyzed the interleukin 8 variants (rs4073, rs2227306, and rs2227307) and their association with the response to inhaled bronchodilators in cystic fibrosis patients.
Methods: Analysis of interleukin 8 gene variants was performed by restriction fragment length polymorphism of polymerase chain reaction. The association between spirometry markers and the response to inhaled bronchodilators was evaluated by Mann‐Whitney and Kruskal‐Wallis tests. The analysis included all cystic fibrosis patients, and subsequently patients with two mutations in the cystic fibrosis transmembrane conductance regulator gene belonging to classes I to III.
Results: This study included 186 cystic fibrosis patients. There was no association of the rs2227307 variant with the response to inhaled bronchodilators. The rs2227306 variant was associated with FEF50% in the dominant group and in the group with two identified mutations in the cystic fibrosis transmembrane conductance regulator gene. The rs4073 variant was associated with spirometry markers in four genetic models: co‐dominant (FEF25‐75% and FEF75%), dominant (FEV1, FEF50%, FEF75%, and FEF25‐75%), recessive (FEF75% and FEF25‐75%), and over‐dominant (FEV1/FVC).
Conclusions: This study highlighted the importance of the rs4073 variant of the interleukin 8 gene, regarding response to inhaled bronchodilators, and of the assessment of mutations in the cystic fibrosis transmembrane conductance regulator gene. |
| first_indexed | 2024-04-12T15:33:14Z |
| format | Article |
| id | doaj.art-2ce0928fa20f4affa7e799ab515469a3 |
| institution | Directory Open Access Journal |
| issn | 2255-5536 |
| language | Portuguese |
| last_indexed | 2024-04-12T15:33:14Z |
| publishDate | 2017-11-01 |
| publisher | Brazilian Society of Pediatrics |
| record_format | Article |
| series | Jornal de Pediatria (Versão em Português) |
| spelling | doaj.art-2ce0928fa20f4affa7e799ab515469a32022-12-22T03:27:01ZporBrazilian Society of PediatricsJornal de Pediatria (Versão em Português)2255-55362017-11-0193663964810.1016/j.jpedp.2017.07.002Variants in the interleukin 8 gene and the response to inhaled bronchodilators in cystic fibrosisLarissa Lazzarini Furlan0José Dirceu Ribeiro1Carmen Sílvia Bertuzzo2João Batista Salomão Junior3Dorotéia Rossi Silva Souza4Fernando Augusto Lima Marson5Faculdade de Medicina de São José do Rio Preto, São José do Rio Preto, SP, BrasilUniversidade Estadual de Campinas (Unicamp), Faculdade de Ciências Médicas, Departamento de Pediatria, Campinas, SP, BrasilUniversidade Estadual de Campinas (Unicamp), Faculdade de Ciências Médicas, Departamento de Genética Médica, Campinas, SP, BrasilFaculdade de Medicina de São José do Rio Preto, Hospital Universitário, Departamento de Pediatria, São José do Rio Preto, SP, BrasilFaculdade de Medicina de São José do Rio Preto, Centro de Pesquisa de Bioquímica e Biologia Molecular, Departamento de Biologia Molecular, São José do Rio Preto, SP, BrasilUniversidade Estadual de Campinas (Unicamp), Faculdade de Ciências Médicas, Departamento de Pediatria, Campinas, SP, BrasilObjective: Interleukin 8 protein promotes inflammatory responses, even in airways. The presence of interleukin 8 gene variants causes altered inflammatory responses and possibly varied responses to inhaled bronchodilators. Thus, this study analyzed the interleukin 8 variants (rs4073, rs2227306, and rs2227307) and their association with the response to inhaled bronchodilators in cystic fibrosis patients. Methods: Analysis of interleukin 8 gene variants was performed by restriction fragment length polymorphism of polymerase chain reaction. The association between spirometry markers and the response to inhaled bronchodilators was evaluated by Mann‐Whitney and Kruskal‐Wallis tests. The analysis included all cystic fibrosis patients, and subsequently patients with two mutations in the cystic fibrosis transmembrane conductance regulator gene belonging to classes I to III. Results: This study included 186 cystic fibrosis patients. There was no association of the rs2227307 variant with the response to inhaled bronchodilators. The rs2227306 variant was associated with FEF50% in the dominant group and in the group with two identified mutations in the cystic fibrosis transmembrane conductance regulator gene. The rs4073 variant was associated with spirometry markers in four genetic models: co‐dominant (FEF25‐75% and FEF75%), dominant (FEV1, FEF50%, FEF75%, and FEF25‐75%), recessive (FEF75% and FEF25‐75%), and over‐dominant (FEV1/FVC). Conclusions: This study highlighted the importance of the rs4073 variant of the interleukin 8 gene, regarding response to inhaled bronchodilators, and of the assessment of mutations in the cystic fibrosis transmembrane conductance regulator gene.http://www.sciencedirect.com/science/article/pii/S2255553617300794CFTRDisease severityInterleukin 8Lung function |
| spellingShingle | Larissa Lazzarini Furlan José Dirceu Ribeiro Carmen Sílvia Bertuzzo João Batista Salomão Junior Dorotéia Rossi Silva Souza Fernando Augusto Lima Marson Variants in the interleukin 8 gene and the response to inhaled bronchodilators in cystic fibrosis Jornal de Pediatria (Versão em Português) CFTR Disease severity Interleukin 8 Lung function |
| title | Variants in the interleukin 8 gene and the response to inhaled bronchodilators in cystic fibrosis |
| title_full | Variants in the interleukin 8 gene and the response to inhaled bronchodilators in cystic fibrosis |
| title_fullStr | Variants in the interleukin 8 gene and the response to inhaled bronchodilators in cystic fibrosis |
| title_full_unstemmed | Variants in the interleukin 8 gene and the response to inhaled bronchodilators in cystic fibrosis |
| title_short | Variants in the interleukin 8 gene and the response to inhaled bronchodilators in cystic fibrosis |
| title_sort | variants in the interleukin 8 gene and the response to inhaled bronchodilators in cystic fibrosis |
| topic | CFTR Disease severity Interleukin 8 Lung function |
| url | http://www.sciencedirect.com/science/article/pii/S2255553617300794 |
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