Polyphenol-Rich Strawberry Extract Protects Human Dermal Fibroblasts against Hydrogen Peroxide Oxidative Damage and Improves Mitochondrial Functionality
Strawberry bioactive compounds are widely known to be powerful antioxidants. In this study, the antioxidant and anti-aging activities of a polyphenol-rich strawberry extract were evaluated using human dermal fibroblasts exposed to H2O2. Firstly, the phenol and flavonoid contents of strawberry extrac...
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MDPI AG
2014-06-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/19/6/7798 |
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| author | Francesca Giampieri José M. Alvarez-Suarez Luca Mazzoni Tamara Y. Forbes-Hernandez Massimiliano Gasparrini Ana M. Gonzàlez-Paramàs Celestino Santos-Buelga José L. Quiles Stefano Bompadre Bruno Mezzetti Maurizio Battino |
| author_facet | Francesca Giampieri José M. Alvarez-Suarez Luca Mazzoni Tamara Y. Forbes-Hernandez Massimiliano Gasparrini Ana M. Gonzàlez-Paramàs Celestino Santos-Buelga José L. Quiles Stefano Bompadre Bruno Mezzetti Maurizio Battino |
| author_sort | Francesca Giampieri |
| collection | DOAJ |
| description | Strawberry bioactive compounds are widely known to be powerful antioxidants. In this study, the antioxidant and anti-aging activities of a polyphenol-rich strawberry extract were evaluated using human dermal fibroblasts exposed to H2O2. Firstly, the phenol and flavonoid contents of strawberry extract were studied, as well as the antioxidant capacity. HPLC-DAD analysis was performed to determine the vitamin C and β-carotene concentration, while HPLC-DAD/ESI-MS analysis was used for anthocyanin identification. Strawberry extract presented a high antioxidant capacity, and a relevant concentration of vitamins and phenolics. Pelargonidin- and cyanidin-glycosides were the most representative anthocyanin components of the fruits. Fibroblasts incubated with strawberry extract and stressed with H2O2 showed an increase in cell viability, a smaller intracellular amount of ROS, and a reduction of membrane lipid peroxidation and DNA damage. Strawberry extract was also able to improve mitochondrial functionality, increasing the basal respiration of mitochondria and to promote a regenerative capacity of cells after exposure to pro-oxidant stimuli. These findings confirm that strawberries possess antioxidant properties and provide new insights into the beneficial role of strawberry bioactive compounds on protecting skin from oxidative stress and aging. |
| first_indexed | 2024-12-11T12:10:34Z |
| format | Article |
| id | doaj.art-2ce25263135844edad1314d3556173d1 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-11T12:10:34Z |
| publishDate | 2014-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-2ce25263135844edad1314d3556173d12022-12-22T01:07:48ZengMDPI AGMolecules1420-30492014-06-011967798781610.3390/molecules19067798molecules19067798Polyphenol-Rich Strawberry Extract Protects Human Dermal Fibroblasts against Hydrogen Peroxide Oxidative Damage and Improves Mitochondrial FunctionalityFrancesca Giampieri0José M. Alvarez-Suarez1Luca Mazzoni2Tamara Y. Forbes-Hernandez3Massimiliano Gasparrini4Ana M. Gonzàlez-Paramàs5Celestino Santos-Buelga6José L. Quiles7Stefano Bompadre8Bruno Mezzetti9Maurizio Battino10Dipartimento di Scienze Agrarie, Alimentari e Ambientali, Università Politecnica delle Marche, Via Ranieri 65, Ancona 60131, ItalyDipartimento di Scienze Agrarie, Alimentari e Ambientali, Università Politecnica delle Marche, Via Ranieri 65, Ancona 60131, ItalyDipartimento di Scienze Cliniche Specialistiche ed Odontostomatologiche (DISCO)-Sez. Biochimica, Facoltà di Medicina, Università Politecnica delle Marche, Ancona 60131, ItalyDipartimento di Scienze Cliniche Specialistiche ed Odontostomatologiche (DISCO)-Sez. Biochimica, Facoltà di Medicina, Università Politecnica delle Marche, Ancona 60131, ItalyDipartimento di Scienze Cliniche Specialistiche ed Odontostomatologiche (DISCO)-Sez. Biochimica, Facoltà di Medicina, Università Politecnica delle Marche, Ancona 60131, ItalyGrupo de Investigación en Polifenoles (GIP-USAL), Faculty of Pharmacy, Salamanca University, Campus Miguel de Unamuno, Salamanca E37007, SpainGrupo de Investigación en Polifenoles (GIP-USAL), Faculty of Pharmacy, Salamanca University, Campus Miguel de Unamuno, Salamanca E37007, SpainDepartment of Physiology, Institute of Nutrition and Food Technology ''José Mataix", Biomedical Research Centre, University of Granada, Granada 18100, SpainDipartimento di Scienze Biomediche e Sanità Pubblica, Facoltà di Medicina, Università Politecnica delle Marche Via Ranieri 65, Ancona 60131, ItalyDipartimento di Scienze Agrarie, Alimentari e Ambientali, Università Politecnica delle Marche, Via Ranieri 65, Ancona 60131, ItalyDipartimento di Scienze Cliniche Specialistiche ed Odontostomatologiche (DISCO)-Sez. Biochimica, Facoltà di Medicina, Università Politecnica delle Marche, Ancona 60131, ItalyStrawberry bioactive compounds are widely known to be powerful antioxidants. In this study, the antioxidant and anti-aging activities of a polyphenol-rich strawberry extract were evaluated using human dermal fibroblasts exposed to H2O2. Firstly, the phenol and flavonoid contents of strawberry extract were studied, as well as the antioxidant capacity. HPLC-DAD analysis was performed to determine the vitamin C and β-carotene concentration, while HPLC-DAD/ESI-MS analysis was used for anthocyanin identification. Strawberry extract presented a high antioxidant capacity, and a relevant concentration of vitamins and phenolics. Pelargonidin- and cyanidin-glycosides were the most representative anthocyanin components of the fruits. Fibroblasts incubated with strawberry extract and stressed with H2O2 showed an increase in cell viability, a smaller intracellular amount of ROS, and a reduction of membrane lipid peroxidation and DNA damage. Strawberry extract was also able to improve mitochondrial functionality, increasing the basal respiration of mitochondria and to promote a regenerative capacity of cells after exposure to pro-oxidant stimuli. These findings confirm that strawberries possess antioxidant properties and provide new insights into the beneficial role of strawberry bioactive compounds on protecting skin from oxidative stress and aging.http://www.mdpi.com/1420-3049/19/6/7798strawberryanthocyaninROSlipid peroxidationDNA damagefibroblasts |
| spellingShingle | Francesca Giampieri José M. Alvarez-Suarez Luca Mazzoni Tamara Y. Forbes-Hernandez Massimiliano Gasparrini Ana M. Gonzàlez-Paramàs Celestino Santos-Buelga José L. Quiles Stefano Bompadre Bruno Mezzetti Maurizio Battino Polyphenol-Rich Strawberry Extract Protects Human Dermal Fibroblasts against Hydrogen Peroxide Oxidative Damage and Improves Mitochondrial Functionality Molecules strawberry anthocyanin ROS lipid peroxidation DNA damage fibroblasts |
| title | Polyphenol-Rich Strawberry Extract Protects Human Dermal Fibroblasts against Hydrogen Peroxide Oxidative Damage and Improves Mitochondrial Functionality |
| title_full | Polyphenol-Rich Strawberry Extract Protects Human Dermal Fibroblasts against Hydrogen Peroxide Oxidative Damage and Improves Mitochondrial Functionality |
| title_fullStr | Polyphenol-Rich Strawberry Extract Protects Human Dermal Fibroblasts against Hydrogen Peroxide Oxidative Damage and Improves Mitochondrial Functionality |
| title_full_unstemmed | Polyphenol-Rich Strawberry Extract Protects Human Dermal Fibroblasts against Hydrogen Peroxide Oxidative Damage and Improves Mitochondrial Functionality |
| title_short | Polyphenol-Rich Strawberry Extract Protects Human Dermal Fibroblasts against Hydrogen Peroxide Oxidative Damage and Improves Mitochondrial Functionality |
| title_sort | polyphenol rich strawberry extract protects human dermal fibroblasts against hydrogen peroxide oxidative damage and improves mitochondrial functionality |
| topic | strawberry anthocyanin ROS lipid peroxidation DNA damage fibroblasts |
| url | http://www.mdpi.com/1420-3049/19/6/7798 |
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