Age-Dependent Vulnerability to Oxidative Stress of Postnatal Rat Pyramidal Motor Cortex Neurons
Oxidative stress is one of the main proposed mechanisms involved in neuronal degeneration. To evaluate the consequences of oxidative stress on motor cortex pyramidal neurons during postnatal development, rats were classified into three groups: Newborn (P2–P7); infantile (P11–P15); and young adult (P...
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| Format: | Article |
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MDPI AG
2020-12-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/9/12/1307 |
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| author | Livia Carrascal Ella Gorton Ricardo Pardillo-Díaz Patricia Perez-García Ricardo Gómez-Oliva Carmen Castro Pedro Nunez-Abades |
| author_facet | Livia Carrascal Ella Gorton Ricardo Pardillo-Díaz Patricia Perez-García Ricardo Gómez-Oliva Carmen Castro Pedro Nunez-Abades |
| author_sort | Livia Carrascal |
| collection | DOAJ |
| description | Oxidative stress is one of the main proposed mechanisms involved in neuronal degeneration. To evaluate the consequences of oxidative stress on motor cortex pyramidal neurons during postnatal development, rats were classified into three groups: Newborn (P2–P7); infantile (P11–P15); and young adult (P20–P40). Oxidative stress was induced by 10 µM of cumene hydroperoxide (CH) application. In newborn rats, using the whole cell patch-clamp technique in brain slices, no significant modifications in membrane excitability were found. In infantile rats, the input resistance increased and rheobase decreased due to the blockage of GABAergic tonic conductance. Lipid peroxidation induced by CH resulted in a noticeable increase in protein-bound 4-hidroxynonenal in homogenates in only infantile and young adult rat slices. Interestingly, homogenates of newborn rat brain slices showed the highest capacity to respond to oxidative stress by dramatically increasing their glutathione and free thiol content. This increase correlated with a time-dependent increase in the glutathione reductase activity, suggesting a greater buffering capacity of newborn rats to resist oxidative stress. Furthermore, pre-treatment of the slices with glutathione monoethyl ester acted as a neuroprotector in pyramidal neurons of infantile rats. We conclude that during maturation, the vulnerability to oxidative stress in rat motor neurons increases with age. |
| first_indexed | 2024-03-10T13:54:59Z |
| format | Article |
| id | doaj.art-2ce9447acbb94805a98a95013caae2ec |
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| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-10T13:54:59Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-2ce9447acbb94805a98a95013caae2ec2023-11-21T01:43:23ZengMDPI AGAntioxidants2076-39212020-12-01912130710.3390/antiox9121307Age-Dependent Vulnerability to Oxidative Stress of Postnatal Rat Pyramidal Motor Cortex NeuronsLivia Carrascal0Ella Gorton1Ricardo Pardillo-Díaz2Patricia Perez-García3Ricardo Gómez-Oliva4Carmen Castro5Pedro Nunez-Abades6Departament of Physiology, Pharmacy School, University of Seville, 41012 Seville, SpainDepartament of Physiology, Pharmacy School, University of Seville, 41012 Seville, SpainBiomedical Research and Innovation Institute of Cadiz (INIBICA), 11003 Cadiz, SpainDepartament of Physiology, Pharmacy School, University of Seville, 41012 Seville, SpainBiomedical Research and Innovation Institute of Cadiz (INIBICA), 11003 Cadiz, SpainBiomedical Research and Innovation Institute of Cadiz (INIBICA), 11003 Cadiz, SpainDepartament of Physiology, Pharmacy School, University of Seville, 41012 Seville, SpainOxidative stress is one of the main proposed mechanisms involved in neuronal degeneration. To evaluate the consequences of oxidative stress on motor cortex pyramidal neurons during postnatal development, rats were classified into three groups: Newborn (P2–P7); infantile (P11–P15); and young adult (P20–P40). Oxidative stress was induced by 10 µM of cumene hydroperoxide (CH) application. In newborn rats, using the whole cell patch-clamp technique in brain slices, no significant modifications in membrane excitability were found. In infantile rats, the input resistance increased and rheobase decreased due to the blockage of GABAergic tonic conductance. Lipid peroxidation induced by CH resulted in a noticeable increase in protein-bound 4-hidroxynonenal in homogenates in only infantile and young adult rat slices. Interestingly, homogenates of newborn rat brain slices showed the highest capacity to respond to oxidative stress by dramatically increasing their glutathione and free thiol content. This increase correlated with a time-dependent increase in the glutathione reductase activity, suggesting a greater buffering capacity of newborn rats to resist oxidative stress. Furthermore, pre-treatment of the slices with glutathione monoethyl ester acted as a neuroprotector in pyramidal neurons of infantile rats. We conclude that during maturation, the vulnerability to oxidative stress in rat motor neurons increases with age.https://www.mdpi.com/2076-3921/9/12/1307oxidative stressmotor cortexglutathione levels in the brainpostnatal developmentmembrane excitabilityamyotrophic lateral sclerosis |
| spellingShingle | Livia Carrascal Ella Gorton Ricardo Pardillo-Díaz Patricia Perez-García Ricardo Gómez-Oliva Carmen Castro Pedro Nunez-Abades Age-Dependent Vulnerability to Oxidative Stress of Postnatal Rat Pyramidal Motor Cortex Neurons Antioxidants oxidative stress motor cortex glutathione levels in the brain postnatal development membrane excitability amyotrophic lateral sclerosis |
| title | Age-Dependent Vulnerability to Oxidative Stress of Postnatal Rat Pyramidal Motor Cortex Neurons |
| title_full | Age-Dependent Vulnerability to Oxidative Stress of Postnatal Rat Pyramidal Motor Cortex Neurons |
| title_fullStr | Age-Dependent Vulnerability to Oxidative Stress of Postnatal Rat Pyramidal Motor Cortex Neurons |
| title_full_unstemmed | Age-Dependent Vulnerability to Oxidative Stress of Postnatal Rat Pyramidal Motor Cortex Neurons |
| title_short | Age-Dependent Vulnerability to Oxidative Stress of Postnatal Rat Pyramidal Motor Cortex Neurons |
| title_sort | age dependent vulnerability to oxidative stress of postnatal rat pyramidal motor cortex neurons |
| topic | oxidative stress motor cortex glutathione levels in the brain postnatal development membrane excitability amyotrophic lateral sclerosis |
| url | https://www.mdpi.com/2076-3921/9/12/1307 |
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