Transcriptomic Analysis Provides Insights to Reveal the bmp6 Function Related to the Development of Intermuscular Bones in Zebrafish
Intermuscular bones (IBs) are small, hard-boned spicules located in the muscle tissue that mainly exist in the myosepta of lower teleosts, which hurt the edibleness and economic value of fish. The study of the development of IBs is very important for freshwater aquaculture fish, but the molecular me...
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| Format: | Article |
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Frontiers Media S.A.
2022-05-01
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| Series: | Frontiers in Cell and Developmental Biology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcell.2022.821471/full |
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| author | Huan Xu Huan Xu Huan Xu Huan Xu Guangxiang Tong Guangxiang Tong Guangxiang Tong Ting Yan Ting Yan Ting Yan Le Dong Le Dong Xiaoxing Yang Xiaoxing Yang Dongyu Dou Zhipeng Sun Zhipeng Sun Zhipeng Sun Tianqi Liu Tianqi Liu Tianqi Liu Xianhu Zheng Xianhu Zheng Xianhu Zheng Jian Yang Xiaowen Sun Xiaowen Sun Xiaowen Sun Yi Zhou Youyi Kuang Youyi Kuang Youyi Kuang |
| author_facet | Huan Xu Huan Xu Huan Xu Huan Xu Guangxiang Tong Guangxiang Tong Guangxiang Tong Ting Yan Ting Yan Ting Yan Le Dong Le Dong Xiaoxing Yang Xiaoxing Yang Dongyu Dou Zhipeng Sun Zhipeng Sun Zhipeng Sun Tianqi Liu Tianqi Liu Tianqi Liu Xianhu Zheng Xianhu Zheng Xianhu Zheng Jian Yang Xiaowen Sun Xiaowen Sun Xiaowen Sun Yi Zhou Youyi Kuang Youyi Kuang Youyi Kuang |
| author_sort | Huan Xu |
| collection | DOAJ |
| description | Intermuscular bones (IBs) are small, hard-boned spicules located in the muscle tissue that mainly exist in the myosepta of lower teleosts, which hurt the edibleness and economic value of fish. The study of the development of IBs is very important for freshwater aquaculture fish, but the molecular mechanism of its formation and the key regulatory genes remain unclear. In this study, we first constructed two types of zebrafish mutants (the mutants losing IBs and the mutants with partial deletion of IBs) by knocking out bmp6. We then carried out a transcriptomic analysis to reveal the role of bmp6 in the developmental mechanism of IBs; we used the caudal musculoskeletal tissues of these mutants and wild-type zebrafish at three development stages (20, 45, and 60 dph) to perform transcriptomic analysis. The results showed that the deficiency of bmp6 upregulated sik1 and activated the TNF-A signaling via the NF-KB pathway, which inhibited the development of osteoblasts and promoted osteoclast formation, thereby inhibiting the formation of IBs. These results provided insights to understand the role of bmp6 in the development of IBs in zebrafish and are useful for selective breeding of IBs in cyprinids. |
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| institution | Directory Open Access Journal |
| issn | 2296-634X |
| language | English |
| last_indexed | 2024-04-14T01:08:59Z |
| publishDate | 2022-05-01 |
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| spelling | doaj.art-2ce953fd59b54bcfa2f8e0301aeedae72022-12-22T02:21:08ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2022-05-011010.3389/fcell.2022.821471821471Transcriptomic Analysis Provides Insights to Reveal the bmp6 Function Related to the Development of Intermuscular Bones in ZebrafishHuan Xu0Huan Xu1Huan Xu2Huan Xu3Guangxiang Tong4Guangxiang Tong5Guangxiang Tong6Ting Yan7Ting Yan8Ting Yan9Le Dong10Le Dong11Xiaoxing Yang12Xiaoxing Yang13Dongyu Dou14Zhipeng Sun15Zhipeng Sun16Zhipeng Sun17Tianqi Liu18Tianqi Liu19Tianqi Liu20Xianhu Zheng21Xianhu Zheng22Xianhu Zheng23Jian Yang24Xiaowen Sun25Xiaowen Sun26Xiaowen Sun27Yi Zhou28Youyi Kuang29Youyi Kuang30Youyi Kuang31Heilongjiang River Fisheries Research Institute of Chinese Academy of Fishery Sciences, Harbin, ChinaNational and Local Joint Engineering Laboratory for Freshwater Fish Breeding, Harbin, ChinaKey Laboratory of Freshwater Aquatic Biotechnology and Breeding, Ministry of Agriculture and Rural Affairs, Harbin, ChinaHeilongjiang Provincial Key Laboratory of Hard Tissue Development and Regeneration, The Second Affiliated Hospital of Harbin Medical University, Harbin, ChinaHeilongjiang River Fisheries Research Institute of Chinese Academy of Fishery Sciences, Harbin, ChinaNational and Local Joint Engineering Laboratory for Freshwater Fish Breeding, Harbin, ChinaKey Laboratory of Freshwater Aquatic Biotechnology and Breeding, Ministry of Agriculture and Rural Affairs, Harbin, ChinaHeilongjiang River Fisheries Research Institute of Chinese Academy of Fishery Sciences, Harbin, ChinaNational and Local Joint Engineering Laboratory for Freshwater Fish Breeding, Harbin, ChinaKey Laboratory of Freshwater Aquatic Biotechnology and Breeding, Ministry of Agriculture and Rural Affairs, Harbin, ChinaHeilongjiang River Fisheries Research Institute of Chinese Academy of Fishery Sciences, Harbin, ChinaCollege of Fisheries and Life Science, Shanghai Ocean University, Shanghai, ChinaHeilongjiang River Fisheries Research Institute of Chinese Academy of Fishery Sciences, Harbin, ChinaCollege of Fisheries and Life Science, Shanghai Ocean University, Shanghai, ChinaHeilongjiang River Fisheries Research Institute of Chinese Academy of Fishery Sciences, Harbin, ChinaHeilongjiang River Fisheries Research Institute of Chinese Academy of Fishery Sciences, Harbin, ChinaNational and Local Joint Engineering Laboratory for Freshwater Fish Breeding, Harbin, ChinaKey Laboratory of Freshwater Aquatic Biotechnology and Breeding, Ministry of Agriculture and Rural Affairs, Harbin, ChinaHeilongjiang River Fisheries Research Institute of Chinese Academy of Fishery Sciences, Harbin, ChinaNational and Local Joint Engineering Laboratory for Freshwater Fish Breeding, Harbin, ChinaKey Laboratory of Freshwater Aquatic Biotechnology and Breeding, Ministry of Agriculture and Rural Affairs, Harbin, ChinaHeilongjiang River Fisheries Research Institute of Chinese Academy of Fishery Sciences, Harbin, ChinaNational and Local Joint Engineering Laboratory for Freshwater Fish Breeding, Harbin, ChinaKey Laboratory of Freshwater Aquatic Biotechnology and Breeding, Ministry of Agriculture and Rural Affairs, Harbin, ChinaInstitute of Mariculture Breeding and Seed Industry, Zhejiang Wanli University, Ningbo, ChinaHeilongjiang River Fisheries Research Institute of Chinese Academy of Fishery Sciences, Harbin, ChinaNational and Local Joint Engineering Laboratory for Freshwater Fish Breeding, Harbin, ChinaKey Laboratory of Freshwater Aquatic Biotechnology and Breeding, Ministry of Agriculture and Rural Affairs, Harbin, ChinaStem Cell Program of Boston Children’s Hospital, Division of Hematology/Oncology, Boston Children’s Hospital and Dana Farber Cancer Institute, Harvard Medical School, Boston, MA, United StatesHeilongjiang River Fisheries Research Institute of Chinese Academy of Fishery Sciences, Harbin, ChinaNational and Local Joint Engineering Laboratory for Freshwater Fish Breeding, Harbin, ChinaKey Laboratory of Freshwater Aquatic Biotechnology and Breeding, Ministry of Agriculture and Rural Affairs, Harbin, ChinaIntermuscular bones (IBs) are small, hard-boned spicules located in the muscle tissue that mainly exist in the myosepta of lower teleosts, which hurt the edibleness and economic value of fish. The study of the development of IBs is very important for freshwater aquaculture fish, but the molecular mechanism of its formation and the key regulatory genes remain unclear. In this study, we first constructed two types of zebrafish mutants (the mutants losing IBs and the mutants with partial deletion of IBs) by knocking out bmp6. We then carried out a transcriptomic analysis to reveal the role of bmp6 in the developmental mechanism of IBs; we used the caudal musculoskeletal tissues of these mutants and wild-type zebrafish at three development stages (20, 45, and 60 dph) to perform transcriptomic analysis. The results showed that the deficiency of bmp6 upregulated sik1 and activated the TNF-A signaling via the NF-KB pathway, which inhibited the development of osteoblasts and promoted osteoclast formation, thereby inhibiting the formation of IBs. These results provided insights to understand the role of bmp6 in the development of IBs in zebrafish and are useful for selective breeding of IBs in cyprinids.https://www.frontiersin.org/articles/10.3389/fcell.2022.821471/fullintermuscular boneszebrafishBMP6bone developmentSIK1 |
| spellingShingle | Huan Xu Huan Xu Huan Xu Huan Xu Guangxiang Tong Guangxiang Tong Guangxiang Tong Ting Yan Ting Yan Ting Yan Le Dong Le Dong Xiaoxing Yang Xiaoxing Yang Dongyu Dou Zhipeng Sun Zhipeng Sun Zhipeng Sun Tianqi Liu Tianqi Liu Tianqi Liu Xianhu Zheng Xianhu Zheng Xianhu Zheng Jian Yang Xiaowen Sun Xiaowen Sun Xiaowen Sun Yi Zhou Youyi Kuang Youyi Kuang Youyi Kuang Transcriptomic Analysis Provides Insights to Reveal the bmp6 Function Related to the Development of Intermuscular Bones in Zebrafish Frontiers in Cell and Developmental Biology intermuscular bones zebrafish BMP6 bone development SIK1 |
| title | Transcriptomic Analysis Provides Insights to Reveal the bmp6 Function Related to the Development of Intermuscular Bones in Zebrafish |
| title_full | Transcriptomic Analysis Provides Insights to Reveal the bmp6 Function Related to the Development of Intermuscular Bones in Zebrafish |
| title_fullStr | Transcriptomic Analysis Provides Insights to Reveal the bmp6 Function Related to the Development of Intermuscular Bones in Zebrafish |
| title_full_unstemmed | Transcriptomic Analysis Provides Insights to Reveal the bmp6 Function Related to the Development of Intermuscular Bones in Zebrafish |
| title_short | Transcriptomic Analysis Provides Insights to Reveal the bmp6 Function Related to the Development of Intermuscular Bones in Zebrafish |
| title_sort | transcriptomic analysis provides insights to reveal the bmp6 function related to the development of intermuscular bones in zebrafish |
| topic | intermuscular bones zebrafish BMP6 bone development SIK1 |
| url | https://www.frontiersin.org/articles/10.3389/fcell.2022.821471/full |
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