In Vitro Effect of Putty Calcium Silicate Materials on Human Periodontal Ligament Stem Cells
New bioactive materials have been developed for retrograde root filling. These materials come into contact with vital tissues and facilitate biomineralization and apical repair. The objective of this study was to evaluate the cytocompatibility and bioactivity of two bioactive cements, Bio-C Repair (...
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| Format: | Article |
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MDPI AG
2020-01-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/10/1/325 |
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| author | Francisco Javier Rodríguez-Lozano Sergio López-García David García-Bernal Miguel R. Pecci-Lloret Julia Guerrero-Gironés María P. Pecci-Lloret Adrián Lozano Carmen Llena Gianrico Spagnuolo Leopoldo Forner |
| author_facet | Francisco Javier Rodríguez-Lozano Sergio López-García David García-Bernal Miguel R. Pecci-Lloret Julia Guerrero-Gironés María P. Pecci-Lloret Adrián Lozano Carmen Llena Gianrico Spagnuolo Leopoldo Forner |
| author_sort | Francisco Javier Rodríguez-Lozano |
| collection | DOAJ |
| description | New bioactive materials have been developed for retrograde root filling. These materials come into contact with vital tissues and facilitate biomineralization and apical repair. The objective of this study was to evaluate the cytocompatibility and bioactivity of two bioactive cements, Bio-C Repair (Angelus, Londrina, Pr, Brazil) and TotalFill BC RRM putty (FGK, Dentaire SA, La-Chaux-de-fonds, Switzerland). The biological properties in human periodontal ligament stem cells (hPDLSCs) that were exposed to Bio-C Repair and TotalFill BC RRM putty were studied. Cell viability, migration, and cell adhesion were analyzed. Moreover, qPCR and mineralization assay were performed to evaluate the bioactivity potential of these cements. The results were statistically analyzed using ANOVA and the Tukey test (<i>p</i> < 0.05). It was observed that cell viability and cell migration in Bio-C Repair and TotalFill BC RRM putty were similar to the control without statistically significant differences, except at 72 h when TotalFill BC RRM putty was slightly lower (<i>p</i> < 0.05). Excellent cell adhesion and morphology were observed with both Bio-C Repair and TotalFill BC RRM putty. Both cements promoted the osteo- and cementogenic differentiation of hPDLSCs. These results suggest that Bio-C Repair and TotalFill BC RRM putty are biologically appropriate materials to be used as retrograde obturation material. |
| first_indexed | 2024-04-12T21:58:06Z |
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| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-04-12T21:58:06Z |
| publishDate | 2020-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-2cec2527bb5342b6bd52959c5e92a3fd2022-12-22T03:15:13ZengMDPI AGApplied Sciences2076-34172020-01-0110132510.3390/app10010325app10010325In Vitro Effect of Putty Calcium Silicate Materials on Human Periodontal Ligament Stem CellsFrancisco Javier Rodríguez-Lozano0Sergio López-García1David García-Bernal2Miguel R. Pecci-Lloret3Julia Guerrero-Gironés4María P. Pecci-Lloret5Adrián Lozano6Carmen Llena7Gianrico Spagnuolo8Leopoldo Forner9Research Group Cellular Therapy and Hematopoietic Transplant, Biomedical Research Institute, Virgen de la Arrixaca Clinical University Hospital, IMIB-Arrixaca, University of Murcia, 30120 Murcia, SpainDepartment of Genetics, Faculty of Biology, University of Murcia, 30100 Murcia, SpainResearch Group Cellular Therapy and Hematopoietic Transplant, Biomedical Research Institute, Virgen de la Arrixaca Clinical University Hospital, IMIB-Arrixaca, University of Murcia, 30120 Murcia, SpainClínica Odontológica Universitaria, Morales Meseguer Hospital, Faculty of Medicine, University of Murcia, 30007 Murcia, SpainClínica Odontológica Universitaria, Morales Meseguer Hospital, Faculty of Medicine, University of Murcia, 30007 Murcia, SpainClínica Odontológica Universitaria, Morales Meseguer Hospital, Faculty of Medicine, University of Murcia, 30007 Murcia, SpainSchool of Dentistry, Faculty of Medicine, University of Valencia, 46010 Valencia, SpainSchool of Dentistry, Faculty of Medicine, University of Valencia, 46010 Valencia, SpainDepartment of Neurosciences, Reproductive and Odontostomatological Sciences, University of Naples “Federico II”, 80138 Napoli, ItalySchool of Dentistry, Faculty of Medicine, University of Valencia, 46010 Valencia, SpainNew bioactive materials have been developed for retrograde root filling. These materials come into contact with vital tissues and facilitate biomineralization and apical repair. The objective of this study was to evaluate the cytocompatibility and bioactivity of two bioactive cements, Bio-C Repair (Angelus, Londrina, Pr, Brazil) and TotalFill BC RRM putty (FGK, Dentaire SA, La-Chaux-de-fonds, Switzerland). The biological properties in human periodontal ligament stem cells (hPDLSCs) that were exposed to Bio-C Repair and TotalFill BC RRM putty were studied. Cell viability, migration, and cell adhesion were analyzed. Moreover, qPCR and mineralization assay were performed to evaluate the bioactivity potential of these cements. The results were statistically analyzed using ANOVA and the Tukey test (<i>p</i> < 0.05). It was observed that cell viability and cell migration in Bio-C Repair and TotalFill BC RRM putty were similar to the control without statistically significant differences, except at 72 h when TotalFill BC RRM putty was slightly lower (<i>p</i> < 0.05). Excellent cell adhesion and morphology were observed with both Bio-C Repair and TotalFill BC RRM putty. Both cements promoted the osteo- and cementogenic differentiation of hPDLSCs. These results suggest that Bio-C Repair and TotalFill BC RRM putty are biologically appropriate materials to be used as retrograde obturation material.https://www.mdpi.com/2076-3417/10/1/325retrograde filling materialsbiocompatibilityapical surgeryperiodontal ligament stem cellsendodontic |
| spellingShingle | Francisco Javier Rodríguez-Lozano Sergio López-García David García-Bernal Miguel R. Pecci-Lloret Julia Guerrero-Gironés María P. Pecci-Lloret Adrián Lozano Carmen Llena Gianrico Spagnuolo Leopoldo Forner In Vitro Effect of Putty Calcium Silicate Materials on Human Periodontal Ligament Stem Cells Applied Sciences retrograde filling materials biocompatibility apical surgery periodontal ligament stem cells endodontic |
| title | In Vitro Effect of Putty Calcium Silicate Materials on Human Periodontal Ligament Stem Cells |
| title_full | In Vitro Effect of Putty Calcium Silicate Materials on Human Periodontal Ligament Stem Cells |
| title_fullStr | In Vitro Effect of Putty Calcium Silicate Materials on Human Periodontal Ligament Stem Cells |
| title_full_unstemmed | In Vitro Effect of Putty Calcium Silicate Materials on Human Periodontal Ligament Stem Cells |
| title_short | In Vitro Effect of Putty Calcium Silicate Materials on Human Periodontal Ligament Stem Cells |
| title_sort | in vitro effect of putty calcium silicate materials on human periodontal ligament stem cells |
| topic | retrograde filling materials biocompatibility apical surgery periodontal ligament stem cells endodontic |
| url | https://www.mdpi.com/2076-3417/10/1/325 |
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