Replication of Human Sapovirus in Human-Induced Pluripotent Stem Cell-Derived Intestinal Epithelial Cells
Sapoviruses, like noroviruses, are single-stranded positive-sense RNA viruses classified in the family <i>Caliciviridae</i> and are recognized as a causative pathogen of diarrhea in infants and the elderly. Like human norovirus, human sapovirus (HuSaV) has long been difficult to replicat...
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MDPI AG
2023-09-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/15/9/1929 |
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| author | Naomi Matsumoto Shiho Kurokawa Shigeyuki Tamiya Yutaka Nakamura Naomi Sakon Shoko Okitsu Hiroshi Ushijima Yoshikazu Yuki Hiroshi Kiyono Shintaro Sato |
| author_facet | Naomi Matsumoto Shiho Kurokawa Shigeyuki Tamiya Yutaka Nakamura Naomi Sakon Shoko Okitsu Hiroshi Ushijima Yoshikazu Yuki Hiroshi Kiyono Shintaro Sato |
| author_sort | Naomi Matsumoto |
| collection | DOAJ |
| description | Sapoviruses, like noroviruses, are single-stranded positive-sense RNA viruses classified in the family <i>Caliciviridae</i> and are recognized as a causative pathogen of diarrhea in infants and the elderly. Like human norovirus, human sapovirus (HuSaV) has long been difficult to replicate in vitro. Recently, it has been reported that HuSaV can be replicated in vitro by using intestinal epithelial cells (IECs) derived from human tissues and cell lines derived from testicular and duodenal cancers. In this study, we report that multiple genotypes of HuSaV can sufficiently infect and replicate in human-induced pluripotent stem cell-derived IECs. We also show that this HuSaV replication system can be used to investigate the conditions for inactivation of HuSaV by heat and alcohol, and the effects of virus neutralization of antisera obtained by immunization with vaccine antigens, under conditions closer to the living environment. The results of this study confirm that HuSaV can also infect and replicate in human normal IECs regardless of their origin and are expected to contribute to future virological studies. |
| first_indexed | 2024-03-10T21:51:16Z |
| format | Article |
| id | doaj.art-2ceea2f522424a2b94665a2dfe4ddce0 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-10T21:51:16Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-2ceea2f522424a2b94665a2dfe4ddce02023-11-19T13:23:43ZengMDPI AGViruses1999-49152023-09-01159192910.3390/v15091929Replication of Human Sapovirus in Human-Induced Pluripotent Stem Cell-Derived Intestinal Epithelial CellsNaomi Matsumoto0Shiho Kurokawa1Shigeyuki Tamiya2Yutaka Nakamura3Naomi Sakon4Shoko Okitsu5Hiroshi Ushijima6Yoshikazu Yuki7Hiroshi Kiyono8Shintaro Sato9Department of Virology, Research Institute for Microbial Diseases, Osaka University, Osaka 565-0871, JapanDepartment of Human Mucosal Vaccinology, Chiba University Hospital, Chiba 260-8670, JapanDepartment of Microbiology and Immunology, School of Pharmaceutical Sciences, Wakayama Medical University, Wakayama 640-8156, JapanDepartment of Microbiology and Immunology, School of Pharmaceutical Sciences, Wakayama Medical University, Wakayama 640-8156, JapanDepartment of Microbiology, Osaka Institute of Public Health, Osaka 537-0025, JapanDivision of Microbiology, Department of Pathology and Microbiology, Nihon University School of Medicine, Tokyo 173-8610, JapanDivision of Microbiology, Department of Pathology and Microbiology, Nihon University School of Medicine, Tokyo 173-8610, JapanDepartment of Human Mucosal Vaccinology, Chiba University Hospital, Chiba 260-8670, JapanDepartment of Human Mucosal Vaccinology, Chiba University Hospital, Chiba 260-8670, JapanDepartment of Virology, Research Institute for Microbial Diseases, Osaka University, Osaka 565-0871, JapanSapoviruses, like noroviruses, are single-stranded positive-sense RNA viruses classified in the family <i>Caliciviridae</i> and are recognized as a causative pathogen of diarrhea in infants and the elderly. Like human norovirus, human sapovirus (HuSaV) has long been difficult to replicate in vitro. Recently, it has been reported that HuSaV can be replicated in vitro by using intestinal epithelial cells (IECs) derived from human tissues and cell lines derived from testicular and duodenal cancers. In this study, we report that multiple genotypes of HuSaV can sufficiently infect and replicate in human-induced pluripotent stem cell-derived IECs. We also show that this HuSaV replication system can be used to investigate the conditions for inactivation of HuSaV by heat and alcohol, and the effects of virus neutralization of antisera obtained by immunization with vaccine antigens, under conditions closer to the living environment. The results of this study confirm that HuSaV can also infect and replicate in human normal IECs regardless of their origin and are expected to contribute to future virological studies.https://www.mdpi.com/1999-4915/15/9/1929human sapovirusintestinal epithelial cellsin vitro replicationhisto-blood group antigensinduced pluripotent stem cell |
| spellingShingle | Naomi Matsumoto Shiho Kurokawa Shigeyuki Tamiya Yutaka Nakamura Naomi Sakon Shoko Okitsu Hiroshi Ushijima Yoshikazu Yuki Hiroshi Kiyono Shintaro Sato Replication of Human Sapovirus in Human-Induced Pluripotent Stem Cell-Derived Intestinal Epithelial Cells Viruses human sapovirus intestinal epithelial cells in vitro replication histo-blood group antigens induced pluripotent stem cell |
| title | Replication of Human Sapovirus in Human-Induced Pluripotent Stem Cell-Derived Intestinal Epithelial Cells |
| title_full | Replication of Human Sapovirus in Human-Induced Pluripotent Stem Cell-Derived Intestinal Epithelial Cells |
| title_fullStr | Replication of Human Sapovirus in Human-Induced Pluripotent Stem Cell-Derived Intestinal Epithelial Cells |
| title_full_unstemmed | Replication of Human Sapovirus in Human-Induced Pluripotent Stem Cell-Derived Intestinal Epithelial Cells |
| title_short | Replication of Human Sapovirus in Human-Induced Pluripotent Stem Cell-Derived Intestinal Epithelial Cells |
| title_sort | replication of human sapovirus in human induced pluripotent stem cell derived intestinal epithelial cells |
| topic | human sapovirus intestinal epithelial cells in vitro replication histo-blood group antigens induced pluripotent stem cell |
| url | https://www.mdpi.com/1999-4915/15/9/1929 |
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