The Impact of Carbohydrate Management on Coleoptile Elongation in Anaerobically Germinating Seeds of Rice (<i>Oryza sativa</i> L.) under Light and Dark Cycles
The ability of rice to elongate coleoptiles under oxygen deprivation is a determinant of anaerobic germination tolerance, critical for successful direct seeding. Most studies on anaerobic coleoptile elongation have been performed under constant darkness or in flooded soils because a drilling method...
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MDPI AG
2023-04-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/12/7/1565 |
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| author | Haru Hirano Takeru Watanabe Mika Fukuda Takeshi Fukao |
| author_facet | Haru Hirano Takeru Watanabe Mika Fukuda Takeshi Fukao |
| author_sort | Haru Hirano |
| collection | DOAJ |
| description | The ability of rice to elongate coleoptiles under oxygen deprivation is a determinant of anaerobic germination tolerance, critical for successful direct seeding. Most studies on anaerobic coleoptile elongation have been performed under constant darkness or in flooded soils because a drilling method was the primary approach for direct seeding of rice. However, aerial seeding is becoming popular, in which seeds which land on flooded soils are exposed to light during the daytime. Here, we investigated physiological mechanisms underlying anaerobic elongation of coleoptiles under light and dark cycles. This study identified two novel varieties, LG and L202, enabling the development of long coleoptiles under oxygen limitation, comparable to well-characterized varieties with strong anaerobic germination tolerance. Germination experiments using these two tolerant and two intolerant varieties, including Takanari and IR64, revealed that light and dark cycles increased coleoptile length in LG, Takanari, and IR64 relative to constant darkness. Interestingly, even in intolerant lines, dramatic starch breakdown and soluble carbohydrate accumulation occurred under oxygen limitation. However, intolerant lines were more susceptible to a representative soluble sugar, glucose, than tolerant lines under oxygen deprivation, suggesting that coleoptile growth can be inhibited in intolerant lines due to hypersensitivity to soluble sugars accumulated in anaerobically germinating seeds. |
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| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-11T05:26:31Z |
| publishDate | 2023-04-01 |
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| spelling | doaj.art-2ceeb6df93834bf38828cec71ccece0e2023-11-17T17:24:25ZengMDPI AGPlants2223-77472023-04-01127156510.3390/plants12071565The Impact of Carbohydrate Management on Coleoptile Elongation in Anaerobically Germinating Seeds of Rice (<i>Oryza sativa</i> L.) under Light and Dark CyclesHaru Hirano0Takeru Watanabe1Mika Fukuda2Takeshi Fukao3Department of Bioscience and Biotechnology, Fukui Prefectural University, Fukui 910-1195, JapanDepartment of Bioscience and Biotechnology, Fukui Prefectural University, Fukui 910-1195, JapanDepartment of Bioscience and Biotechnology, Fukui Prefectural University, Fukui 910-1195, JapanDepartment of Bioscience and Biotechnology, Fukui Prefectural University, Fukui 910-1195, JapanThe ability of rice to elongate coleoptiles under oxygen deprivation is a determinant of anaerobic germination tolerance, critical for successful direct seeding. Most studies on anaerobic coleoptile elongation have been performed under constant darkness or in flooded soils because a drilling method was the primary approach for direct seeding of rice. However, aerial seeding is becoming popular, in which seeds which land on flooded soils are exposed to light during the daytime. Here, we investigated physiological mechanisms underlying anaerobic elongation of coleoptiles under light and dark cycles. This study identified two novel varieties, LG and L202, enabling the development of long coleoptiles under oxygen limitation, comparable to well-characterized varieties with strong anaerobic germination tolerance. Germination experiments using these two tolerant and two intolerant varieties, including Takanari and IR64, revealed that light and dark cycles increased coleoptile length in LG, Takanari, and IR64 relative to constant darkness. Interestingly, even in intolerant lines, dramatic starch breakdown and soluble carbohydrate accumulation occurred under oxygen limitation. However, intolerant lines were more susceptible to a representative soluble sugar, glucose, than tolerant lines under oxygen deprivation, suggesting that coleoptile growth can be inhibited in intolerant lines due to hypersensitivity to soluble sugars accumulated in anaerobically germinating seeds.https://www.mdpi.com/2223-7747/12/7/1565anaerobic germinationcoleoptile elongation<i>Oryza sativa</i>oxygen deprivationsubmergencesugar sensitivity |
| spellingShingle | Haru Hirano Takeru Watanabe Mika Fukuda Takeshi Fukao The Impact of Carbohydrate Management on Coleoptile Elongation in Anaerobically Germinating Seeds of Rice (<i>Oryza sativa</i> L.) under Light and Dark Cycles Plants anaerobic germination coleoptile elongation <i>Oryza sativa</i> oxygen deprivation submergence sugar sensitivity |
| title | The Impact of Carbohydrate Management on Coleoptile Elongation in Anaerobically Germinating Seeds of Rice (<i>Oryza sativa</i> L.) under Light and Dark Cycles |
| title_full | The Impact of Carbohydrate Management on Coleoptile Elongation in Anaerobically Germinating Seeds of Rice (<i>Oryza sativa</i> L.) under Light and Dark Cycles |
| title_fullStr | The Impact of Carbohydrate Management on Coleoptile Elongation in Anaerobically Germinating Seeds of Rice (<i>Oryza sativa</i> L.) under Light and Dark Cycles |
| title_full_unstemmed | The Impact of Carbohydrate Management on Coleoptile Elongation in Anaerobically Germinating Seeds of Rice (<i>Oryza sativa</i> L.) under Light and Dark Cycles |
| title_short | The Impact of Carbohydrate Management on Coleoptile Elongation in Anaerobically Germinating Seeds of Rice (<i>Oryza sativa</i> L.) under Light and Dark Cycles |
| title_sort | impact of carbohydrate management on coleoptile elongation in anaerobically germinating seeds of rice i oryza sativa i l under light and dark cycles |
| topic | anaerobic germination coleoptile elongation <i>Oryza sativa</i> oxygen deprivation submergence sugar sensitivity |
| url | https://www.mdpi.com/2223-7747/12/7/1565 |
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