Immune Checkpoint Pathway Expression in Lymphocyte Subpopulations in Patients with Common Variable Immunodeficiency and Chronic Lymphocytic Leukemia
This study aims to gain a deeper understanding of chronic lymphocytic leukemia (CLL) and common variable immunodeficiency (CVID) by studying immune cells and specific immune checkpoint signaling pathways. The analysis of the percentage of selected immune points and their ligands (PD-1/PD-L1, CTLA-4/...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-10-01
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| Series: | Cancers |
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| Online Access: | https://www.mdpi.com/2072-6694/15/21/5184 |
| _version_ | 1797632122268155904 |
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| author | Paulina Mertowska Sebastian Mertowski Konrad Smolak Aleksandra Kita Gabriela Kita Katarzyna Guz Marcin Pasiarski Ewelina Grywalska |
| author_facet | Paulina Mertowska Sebastian Mertowski Konrad Smolak Aleksandra Kita Gabriela Kita Katarzyna Guz Marcin Pasiarski Ewelina Grywalska |
| author_sort | Paulina Mertowska |
| collection | DOAJ |
| description | This study aims to gain a deeper understanding of chronic lymphocytic leukemia (CLL) and common variable immunodeficiency (CVID) by studying immune cells and specific immune checkpoint signaling pathways. The analysis of the percentage of selected immune points and their ligands (PD-1/PD-L1, CTLA-4/CD86, and CD200R/CD200) on peripheral blood lymphocyte subpopulations was performed using flow cytometry, and additional analyses determining the serum concentration of the above-mentioned molecules were performed using enzyme immunoassay tests. The obtained results indicate several significant changes in the percentage of almost all tested molecules on selected subpopulations of T and B lymphocytes in both CVID and CLL patients in relation to healthy volunteers and between the disease subunits themselves. The results obtained were also supported by the analysis of the serum concentration of soluble molecules tested. By uncovering valuable insights, we hope to enhance our comprehension and management of these conditions, considering both immunodeficiencies and hematological malignancies. Understanding the role of these signaling pathways in disease development and progression may lead to the development of modern, personalized diagnostic and therapeutic strategies. Ultimately, this knowledge may enable the monitoring of the immune system in patients with CVID and CLL, paving the way for improved patient care in the future. |
| first_indexed | 2024-03-11T11:32:25Z |
| format | Article |
| id | doaj.art-2cf0af5245cc4753bc5ff2881f84b73f |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-11T11:32:25Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-2cf0af5245cc4753bc5ff2881f84b73f2023-11-10T15:00:10ZengMDPI AGCancers2072-66942023-10-011521518410.3390/cancers15215184Immune Checkpoint Pathway Expression in Lymphocyte Subpopulations in Patients with Common Variable Immunodeficiency and Chronic Lymphocytic LeukemiaPaulina Mertowska0Sebastian Mertowski1Konrad Smolak2Aleksandra Kita3Gabriela Kita4Katarzyna Guz5Marcin Pasiarski6Ewelina Grywalska7Department of Experimental Immunology, Medical University of Lublin, 20-093 Lublin, PolandDepartment of Experimental Immunology, Medical University of Lublin, 20-093 Lublin, PolandDepartment of Experimental Immunology, Medical University of Lublin, 20-093 Lublin, PolandDepartment of Experimental Immunology, Medical University of Lublin, 20-093 Lublin, PolandDepartment of Experimental Immunology, Medical University of Lublin, 20-093 Lublin, PolandDepartment of Experimental Immunology, Medical University of Lublin, 20-093 Lublin, PolandDepartment of Immunology, Faculty of Health Sciences, Jan Kochanowski University, 25-317 Kielce, PolandDepartment of Experimental Immunology, Medical University of Lublin, 20-093 Lublin, PolandThis study aims to gain a deeper understanding of chronic lymphocytic leukemia (CLL) and common variable immunodeficiency (CVID) by studying immune cells and specific immune checkpoint signaling pathways. The analysis of the percentage of selected immune points and their ligands (PD-1/PD-L1, CTLA-4/CD86, and CD200R/CD200) on peripheral blood lymphocyte subpopulations was performed using flow cytometry, and additional analyses determining the serum concentration of the above-mentioned molecules were performed using enzyme immunoassay tests. The obtained results indicate several significant changes in the percentage of almost all tested molecules on selected subpopulations of T and B lymphocytes in both CVID and CLL patients in relation to healthy volunteers and between the disease subunits themselves. The results obtained were also supported by the analysis of the serum concentration of soluble molecules tested. By uncovering valuable insights, we hope to enhance our comprehension and management of these conditions, considering both immunodeficiencies and hematological malignancies. Understanding the role of these signaling pathways in disease development and progression may lead to the development of modern, personalized diagnostic and therapeutic strategies. Ultimately, this knowledge may enable the monitoring of the immune system in patients with CVID and CLL, paving the way for improved patient care in the future.https://www.mdpi.com/2072-6694/15/21/5184CTLA-4PD-1PD-L1CD86CD200CD200R |
| spellingShingle | Paulina Mertowska Sebastian Mertowski Konrad Smolak Aleksandra Kita Gabriela Kita Katarzyna Guz Marcin Pasiarski Ewelina Grywalska Immune Checkpoint Pathway Expression in Lymphocyte Subpopulations in Patients with Common Variable Immunodeficiency and Chronic Lymphocytic Leukemia Cancers CTLA-4 PD-1 PD-L1 CD86 CD200 CD200R |
| title | Immune Checkpoint Pathway Expression in Lymphocyte Subpopulations in Patients with Common Variable Immunodeficiency and Chronic Lymphocytic Leukemia |
| title_full | Immune Checkpoint Pathway Expression in Lymphocyte Subpopulations in Patients with Common Variable Immunodeficiency and Chronic Lymphocytic Leukemia |
| title_fullStr | Immune Checkpoint Pathway Expression in Lymphocyte Subpopulations in Patients with Common Variable Immunodeficiency and Chronic Lymphocytic Leukemia |
| title_full_unstemmed | Immune Checkpoint Pathway Expression in Lymphocyte Subpopulations in Patients with Common Variable Immunodeficiency and Chronic Lymphocytic Leukemia |
| title_short | Immune Checkpoint Pathway Expression in Lymphocyte Subpopulations in Patients with Common Variable Immunodeficiency and Chronic Lymphocytic Leukemia |
| title_sort | immune checkpoint pathway expression in lymphocyte subpopulations in patients with common variable immunodeficiency and chronic lymphocytic leukemia |
| topic | CTLA-4 PD-1 PD-L1 CD86 CD200 CD200R |
| url | https://www.mdpi.com/2072-6694/15/21/5184 |
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